TTBK1-IN-1
Based on 2 publication(s) in Google Scholar
TTBK1-IN-1 is a potent, selective and brain-penetrant tau tubulin kinase 1 (TTBK1) inhibitor with an IC50 of 2.7 nM. TTBK1-IN-1 can be used for the research of alzheimer’s disease and related tauopathies. TTBK1-IN-1 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 99.17%
- CAS No.: 2735015-60-6
- Formula: C18H19N5O2
- Molecular Weight:337.38
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) TTBK1-IN-1
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | IC50 |
10 μM
Compound: 31
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Cytotoxicity against human HepG2 cells in galactose containing media incubated for 72 hrs by celltiter-glo luminescent cell viability assay
Cytotoxicity against human HepG2 cells in galactose containing media incubated for 72 hrs by celltiter-glo luminescent cell viability assay
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[PMID: 33944571] |
| HepG2 | IC50 |
8.9 μM
Compound: 31
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Cytotoxicity against human HepG2 cells in glucose containing media incubated for 72 hrs by celltiter-glo luminescent cell viability assay
Cytotoxicity against human HepG2 cells in glucose containing media incubated for 72 hrs by celltiter-glo luminescent cell viability assay
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[PMID: 33944571] |
In Vitro
In primary mouse neuronal cultures at DIV14, and analysis of neurite branching is used as a surrogate of neuron health. At acute timepoints (up to 1 h following administration), TTBK1-IN-1 (0.0625-100 μM; 1 hour) has no effect on neurite length when tested up to 100 μM. At chronic effects test, TTBK1-IN-1 (0.0625-100 μM; 6-24 hours) cause a significant decrease in neurite length at the 50 and 100 μM concentrations beginning at 12–18 h following treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2735015-60-6
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Appearance Solid
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Molecular Weight 337.38
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Formula C18H19N5O2
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Color White to off-white
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SMILES
O[C@@](C)(CC)C#CC1=CC2=C(C=CN2C3=CC(OC)=NC(N)=N3)C=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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SLAS Discov
Identification of Tau-Tubulin Kinase 1 Inhibitors by Microfluidics-based Mobility Shift Assay from a Kinase Inhibitor Library. [Abstract]2023 Dec;28(8):385-393. PMID: 37399991 -
bioRxiv
2026 Apr 28:2026.04.27.721186. PMID: 42094407
Solvent & Solubility
In Vitro:
Ethanol : 33.33 mg/mL (98.79 mM; ultrasonic and adjust pH to 3 with HCl)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.9640 mL | 14.8201 mL | 29.6402 mL | 74.1004 mL |
| 5 mM | 0.5928 mL | 2.9640 mL | 5.9280 mL | 14.8201 mL | |
| 10 mM | 0.2964 mL | 1.4820 mL | 2.9640 mL | 7.4100 mL | |
| 15 mM | 0.1976 mL | 0.9880 mL | 1.9760 mL | 4.9400 mL | |
| 20 mM | 0.1482 mL | 0.7410 mL | 1.4820 mL | 3.7050 mL | |
| 25 mM | 0.1186 mL | 0.5928 mL | 1.1856 mL | 2.9640 mL | |
| 30 mM | 0.0988 mL | 0.4940 mL | 0.9880 mL | 2.4700 mL | |
| 40 mM | 0.0741 mL | 0.3705 mL | 0.7410 mL | 1.8525 mL | |
| 50 mM | 0.0593 mL | 0.2964 mL | 0.5928 mL | 1.4820 mL | |
| 60 mM | 0.0494 mL | 0.2470 mL | 0.4940 mL | 1.2350 mL | |
| 80 mM | 0.0371 mL | 0.1853 mL | 0.3705 mL | 0.9263 mL |
TTBK1-IN-1 Related Classifications
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