Tubulysin B
Based on 1 Customer Validation
Tubulysin B is a highly cytotoxic peptide and potent microtubule destabilizing agents isolated from the myxobacteria Archangium geophyra and Angiococcus disciformis. Tubulysin B has IC50 values in the picomolar range against many cancer cell lines, including those with multidrug resistant properties.Tubulysin B is a cytotoxic activity tubulysin which inhibits tubulin polymerization and leads to cell cycle arrest and apoptosis.
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- Pureté : 99.88%
- CAS No.: 205304-87-6
- Formule: C42H63N5O10S
- Masse moléculaire:830.04
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Stockage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB | IC50 |
2 nM
Compound: 2a
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Growth inhibition of human KB cells by [3H]thymidine incorporation assay
Growth inhibition of human KB cells by [3H]thymidine incorporation assay
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[PMID: 21974954] |
In Vitro
Tubulysin B has IC50s of 0.6 and 0.9 nM against KB and A549 tumors cell lines, respectively.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Apoptosis Solutions
- Protocol for Cell Cycle
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
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CAS No. 205304-87-6
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Appearance Solid
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Masse moléculaire 830.04
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Formule C42H63N5O10S
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Color White to off-white
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SMILES
CC(O[C@@H](C1=NC(C(N[C@H](C[C@H](C)C(O)=O)CC2=CC=C(O)C=C2)=O)=CS1)C[C@H](C(C)C)N(COC(CCC)=O)C([C@@]([C@@H](C)CC)([H])NC([C@@H](CCCC3)N3C)=O)=O)=O
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
In Vitro:
DMSO : ≥ 100 mg/mL (120.48 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Leamon CP, et al. Prostate-Specific Membrane Antigen-Specific Antitumor Activity of a Self-Immolative Tubulysin Conjugate. Bioconjug Chem. 2019 Jun 19;30(6):1805-1813. [Content Brief]
[2]. Vlahov IR, et al. Acid mediated formation of an N-acyliminium ion from tubulysins: a new methodology for the synthesis of natural tubulysins and their analogs. Bioorg Med Chem Lett. 2011 Nov 15;21(22):6778-81. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2048 mL | 6.0238 mL | 12.0476 mL | 30.1190 mL |
| 5 mM | 0.2410 mL | 1.2048 mL | 2.4095 mL | 6.0238 mL | |
| 10 mM | 0.1205 mL | 0.6024 mL | 1.2048 mL | 3.0119 mL | |
| 15 mM | 0.0803 mL | 0.4016 mL | 0.8032 mL | 2.0079 mL | |
| 20 mM | 0.0602 mL | 0.3012 mL | 0.6024 mL | 1.5060 mL | |
| 25 mM | 0.0482 mL | 0.2410 mL | 0.4819 mL | 1.2048 mL | |
| 30 mM | 0.0402 mL | 0.2008 mL | 0.4016 mL | 1.0040 mL | |
| 40 mM | 0.0301 mL | 0.1506 mL | 0.3012 mL | 0.7530 mL | |
| 50 mM | 0.0241 mL | 0.1205 mL | 0.2410 mL | 0.6024 mL | |
| 60 mM | 0.0201 mL | 0.1004 mL | 0.2008 mL | 0.5020 mL | |
| 80 mM | 0.0151 mL | 0.0753 mL | 0.1506 mL | 0.3765 mL | |
| 100 mM | 0.0120 mL | 0.0602 mL | 0.1205 mL | 0.3012 mL |
Tubulysin B Related Classifications
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