2. MAPK/ERK Pathway Stem Cell/Wnt Apoptosis Immunology/Inflammation PI3K/Akt/mTOR
  3. p38 MAPK JNK ERK TNF Receptor Interleukin Related PI3K Akt
  4. Umckalin

Umckalin is Coumarin (HY-N0709) derivative that exhibits anti-inflammatory properties. Umckalin reduces phosphorylation of p38 MAPK, JNK, and ERK, lowers TNF-α, IL-6, IL-1β, NO, and PGE2 production. Umckalin can be used for the research of chronic inflammatory diseases.

For research use only. We do not sell to patients.

Umckalin

Umckalin Chemical Structure

CAS No. : 43053-62-9

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Umckalin Related Antibodies

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

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JNK1 JNK2 JNK3 JNK

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ERK ERK1 ERK2 ERK5 ERK8

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TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Akt Akt1 Akt2 Akt3

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Description

Umckalin is Coumarin (HY-N0709) derivative that exhibits anti-inflammatory properties. Umckalin reduces phosphorylation of p38 MAPK, JNK, and ERK, lowers TNF-α, IL-6, IL-1β, NO, and PGE2 production. Umckalin can be used for the research of chronic inflammatory diseases[1][2].

In Vitro

Umckalin (25-400 µM; 72 h) does not reduce viability of B16F10 murine melanoma cells at concentrations up to 200 µM[2].
Umckalin (50-200 µM; 72 h) dose-dependently increases intracellular melanin content in B16F10 murine melanoma cells[2].
Umckalin (50-200 µM; 72 h) dose-dependently enhances tyrosinase activity in B16F10 murine melanoma cells[2].
Umckalin (50-200 µM; 24 h) upregulates TRP-1 and MITF protein expression in B16F10 murine melanoma cells, while having minimal to no effect on tyrosinase and TRP-2 expression[2].
Umckalin (50-200 µM; 24 h) activates the GSK-3β/β-catenin pathway in B16F10 murine melanoma cells by increasing Ser9 phosphorylation of GSK-3β, reducing β-catenin phosphorylation, and increasing total β-catenin levels[2].
Umckalin (50-200 µM; 4 h) suppresses MAPK pathway activity in B16F10 murine melanoma cells by reducing ERK and JNK phosphorylation, while not affecting p38 phosphorylation[2].
Umckalin (50-200 µM; 4 h) activates the PI3K/AKT pathway in B16F10 murine melanoma cells by increasing PI3K and AKT phosphorylationn[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Umckalin Related Antibodies

Cell Viability Assay[2]

Cell Line: B16F10 murine melanoma cells
Concentration: 25; 50; 100; 200; 400 µM
Incubation Time: 72 h
Result: Showed no cytotoxic effects at concentrations up to 200 µM.
Caused significant cytotoxicity at 400 µM.

Western Blot Analysis[2]

Cell Line: B16F10 murine melanoma cells
Concentration: 50; 100; 200 µM
Incubation Time: 24 h
Result: Increased TRP-1 protein expression significantly in a dose-dependent manner (p < 0.001 at all concentrations).
Caused a small but significant increase in tyrosinase expression only at 200 µM.
Had no notable effect on TRP-2 expression.
Increased MITF protein expression significantly in a dose-dependent manner.
Increased Ser9 phosphorylation of GSK-3β significantly in a dose-dependent manner.
Increased total β-catenin levels significantly only at 200 µM.
Reduced β-catenin phosphorylation significantly at 200 µM.

Western Blot Analysis[2]

Cell Line: B16F10 murine melanoma cells
Concentration: 50-200 µM
Incubation Time: 4 h
Result: Reduced ERK phosphorylation significantly in a dose-dependent manner.
Reduced JNK phosphorylation significantly in a dose-dependent manner.
Had no significant effect on p38 phosphorylation.\nIncreased PI3K phosphorylation significantly in a dose-dependent manner.
Increased AKT phosphorylation significantly in a dose-dependent manner.
Molecular Weight

222.19

Formula

C11H10O5
CAS No.
SMILES

O=C1C=CC2=C(OC)C(OC)=C(O)C=C2O1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Umckalin Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Umckalin43053-62-9p38 MAPKJNKERKTNF ReceptorInterleukin RelatedPI3KAktc-Jun N-terminal kinaseExtracellular signal regulated kinasesTumor Necrosis Factor ReceptorTNFRILPhosphoinositide 3-kinasePKBProtein kinase BIL-6iNOSMAPK pathwayB16F10 murine melanoma cellsTNF-αIL-1βCOX-2Inhibitorinhibitorinhibit

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