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Cat. No.: HY-N21699

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Uralione E

Uralione E is a naturally occurring polycyclic polyprenylated acylphloroglucinol (PPAP). Uralione E exhibits a protective effect against corticosterone (HY-B1618)-induced damage in PC12 cells. In a free fatty acid (FFA)-induced L02 cell model, Uralione E dose-dependently reduces intracellular lipid accumulation, downregulates the expression of FASN and CD36, and upregulates the expression of ACOX1 and PPARα. Uralione E can be used in studies related to neuronal cell damage, lipid metabolism, and non-alcoholic steatohepatitis.

For research use only. We do not sell to patients.

CAS No.: 1927024-53-0
Formula: C₃₈H₅₀O₅
Molecular Weight:586.80

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Uralione E Chemical Structure

CAS No.: 1927024-53-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Protocols (3)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

All PPAR Isoforms :

In Vitro

Uralione E (0.1-10.0 μM; 48 h) protects PC12 cells from corticosterone-induced injury, with cell viability reaching 86.6% at 10.0 μM after 48 h of co-treatment^[1].
Uralione E (compound 20) (5-10 μM; 48 h) reduces intracellular lipid accumulation in FFA-induced L02 cells in a dose-dependent manner at 5 μM and 10 μM over 48 h^[2].
Uralione E (compound 20) regulates lipid metabolism-related gene expression in FFA-induced L02 cells, suppressing CD36 and FASN while upregulating PPARα and ACOX1^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Cell Viability Assay^[1]

View Protocol

Cell Line:

rat pheochromocytoma PC12 cells
Concentration:

0.1, 0.3, 1.0, 3.0, 10.0 μM (co-incubated with 200 μM Corticosterone)
Incubation Time:

48 h
Result:

Exerted significant protective effects against corticosterone-induced PC12 cell injury.
Showed cell viability of 82.9% at 0.1 μM.
Showed cell viability of 72.8% at 0.3 μM.
Showed cell viability of 68.1% at 1.0 μM.
Showed cell viability of 78.0% at 3.0 μM.
Showed cell viability of 86.6% at 10.0 μM.
Increased cell viability substantially compared to the injured model group which had 55.6% viability.

Chemical Information

CAS No. 1927024-53-0
Molecular Weight 586.80
Formula C₃₈H₅₀O₅
SMILES

O=C(C1=CC=CC=C1)[C@@]23[C@@](C)([C@H](C[C@]4(C(O[C@H](C4)C(C)(O)C)=C(C3=O)C/C=C(C)\C)C2=O)C/C=C(C)\C)CC/C=C(C)/C
Structure Classification
- Terpenoids
- Other Terpenoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology Molecular Biology Histomorphology

3T3-L1 preadipocyte-to-adipocyte differentiation
3T3-L1 preadipocytes are induced to differentiate after growth arrest using adipogenic media containing insulin, dexamethasone, and IBMX; differentiation is assessed by lipid-droplet accumulation, triglyceride increase, Oil Red O staining, and adipocyte-marker induction such as PPARγ and C/EBPα.
RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Lipid Droplets: Oil Red O/Sudan Dye Lipid Staining
Lipid droplets are intracellular organelles with a neutral-lipid core that stores triacylglycerols and sterol esters, and Oil Red O or Sudan dyes detect these hydrophobic lipid deposits by partitioning into retained lipids in fresh or frozen specimens. Oil Red O stains neutral triglycerides and lipids in frozen tissue sections or air-dried cytologic preparations, while Sudan Black B has also been used as a histochemical fat stain for lipid-rich tissue structures.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhou ZB, et al. Polycyclic Polyprenylated Derivatives from Hypericum uralum: Neuroprotective Effects and Antidepressant-like Activity of Uralodin A. Journal of natural products. 2016 May 27;79(5):1231-40.

[2]. Lu WJ, et al. Diverse Polycyclic Polyprenylated Acylphloroglucinol Congeners with Anti-Nonalcoholic Steatohepatitis Activity from Hypericum forrestii. J Nat Prod. 2021 Apr 23;84(4):1135-1148.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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