Cat. No.: HY-N0862

Harringtonine

18 Cited Publications

Customer Review

Based on 18 publication(s) in Google Scholar

Harringtonine is a natural Cephalotaxus alkaloid that inhibits protein synthesis. Harringtonine has anti-chikungunya virus (CHIKV) activities with an EC₅₀ of 0.24 μM.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 26833-85-2
Formula: C₂₈H₃₇NO₉
Molecular Weight:531.59

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Harringtonine Chemical Structure

CAS No.: 26833-85-2

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (18)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Harringtonine is a natural Cephalotaxus alkaloid that inhibits protein synthesis. Harringtonine has anti-chikungunya virus (CHIKV) activities with an EC₅₀ of 0.24 μM.

Cellular Effect

Cell Line	Type	Value	Description	References
BHK-21	EC₅₀	0.24 μM Compound: 22	Antiviral activity against Chikungunya virus infected in BHK21 cells assessed as inhibition of viral replication after 24 hrs Antiviral activity against Chikungunya virus infected in BHK21 cells assessed as inhibition of viral replication after 24 hrs	[PMID: 24079775]
HeLa	IC₅₀	4 μM Compound: HT	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay	[PMID: 35148094]
HL-60	IC₅₀	0.047 μM Compound: HT	Antiproliferative activity against human HL60 cells after 62 hrs by MTS assay Antiproliferative activity against human HL60 cells after 62 hrs by MTS assay	[PMID: 29286665]
HL-60	IC₅₀	50.5 nM Compound: HT	Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay	[PMID: 35148094]
P388	IC₅₀	0.032 μg/mL Compound: 3	Antileukemic activity against P-388 leukemia cells. Antileukemic activity against P-388 leukemia cells.	10.1016/0960-894X(96)00302-2

In Vitro

Harringtonine inhibits the elongation phase of translation by preventing substrate binding to the acceptor site on the 60-S ribosome subunit and therefore block aminoacyl-tRNA binding and peptide bond formation^[1]. Harringtonine displays potent inhibition of Chikungunya virus infection with an EC₅₀ of 0.24 μM. Harringtonine could inhibit other alphaviruses^[2]. Harringtonine inhibits the growth of human myeloid leukemia cells in vitro at low concentrations. The mechanism of the antitumor action of harringtonine is considered to be an effect on protein synthesis and is characterized by breakdown of polysomes to monosomes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 26833-85-2
Appearance Solid
Molecular Weight 531.59
Formula C₂₈H₃₇NO₉
Color White to light yellow
SMILES

O=C([C@@](CC(OC)=O)(O)CCC(C)(O)C)O[C@H]1[C@](C2=CC(OCO3)=C3C=C2CCN4CCC5)([H])[C@]45C=C1OC
Structure Classification
- Alkaloids
- Pyrrole Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (18)

All Bio/Physico-chemical Assay

Sort by:

Journal Impact Factor

Cell Res

SMYD5 is a ribosomal methyltransferase that catalyzes RPL40 lysine methylation to enhance translation output and promote hepatocellular carcinoma. [Abstract]

2024 Sep;34(9):648-660. PMID: 39103523
Nat Metab

NK cell-derived IFNγ mobilizes free fatty acids from adipose tissue to promote early B cell activation during viral infection. [Abstract]

2025 Apr 11. PMID: 40217117
Nat Immunol

Cathepsin L-dependent positive selection shapes clonal composition and functional fitness of CD4+ T cells. [Abstract]

2025 Jul;26(7):1127-1138. PMID: 40514418
Nat Immunol

An IFNγ-dependent immune-endocrine circuit lowers blood glucose to potentiate the innate antiviral immune response. [Abstract]

2024 Jun;25(6):981-993. PMID: 38811816
Cancer Res

Caloric Restriction Enhances the Efficacy of Anti-Androgen Therapy in Prostate Cancer by Inhibiting Androgen Receptor Translation. [Abstract]

2025 Aug 8. PMID: 40779415

Harringtonine purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Aug 8. [Abstract]

Cells were treated with 2 µg/ml harringtonine (HT) for 3 minutes before cell lysate preparation. The total transcript level for AR mRNA in each fraction was shown in the bar graphs and the percentage shift was calculated.
Nat Commun

ALKBH8-mediated codon-specific translation promotes colorectal tumorigenesis. [Abstract]

2025 Oct 13;16(1):9075. PMID: 41083459

Harringtonine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Oct 13;16(1):9075. [Abstract]

Ribosomal elongation speed was measured using harringtonine chase. Polysome profiles of CTL cells or A8-KO cells treated with harringtonine (2 μg/mL; 0–5 min) for indicated times were obtained.
Nat Commun

Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts. [Abstract]

2024 Aug 26;15(1):7061. PMID: 39187487

Harringtonine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 26;15(1):7061. [Abstract]

Harringtonine (2 μg/mL; 60–180 s). Smoothed metagene plots for a ribosome run-off assay with harringtonine (HAR) chase were generated.

Harringtonine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 26;15(1):7061. [Abstract]

Harringtonine (2 μg/mL; 60–180 s).The expansion of the ribosome-free area over time by harringtonine chase. Linear regression revealed that the global elongation rate was 4.1 codon/s.
Nucleic Acids Res

SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein-RNA interactions with high accuracy. [Abstract]

2019 Apr 8;47(6):e33. PMID: 30715466

Harringtonine purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2019 Apr 8;47(6):e33. [Abstract]

HAR: clusters identified upon acute translation suppression by Harringtonine (2 μg/ml; 1 h). Distribution of AGO2 SpyCLIP clusters in different regions of protein-coding genes and distribution of AGO2 SpyCLIP clusters in different genomic regions corrected by region size.
Cell Death Differ

SOCS2-enhanced ubiquitination of SLC7A11 promotes ferroptosis and radiosensitization in hepatocellular carcinoma. [Abstract]

2023 Jan;30(1):137-151. PMID: 35995846
Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies. [Abstract]

2021 Feb 16;118(7):e2014457118. PMID: 33568529
FASEB J

C81-evoked inhibition of the TNFR1-NFκB pathway during inflammatory processes for stabilization of the impaired vascular endothelial barrier for leukocytes. [Abstract]

2021 Jun;35(6):e21656. PMID: 34042211
J Anim Sci

Glucose inhibits the inflammatory response in goose fatty liver by increasing the ubiquitination level of PKA. [Abstract]

2024 Aug 19:skae239. PMID: 39158360
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
bioRxiv

Nascent protein retention at polysomes reduces kinetic barriers to self-assembly. [Abstract]

2026 May 19:2026.05.17.725791. PMID: 42239437
Biomed Pharmacother

(Homo-)harringtonine prevents endothelial inflammation through IRF-1 dependent downregulation of VCAM1 mRNA expression and inhibition of cell adhesion molecule protein biosynthesis. [Abstract]

2024 Jun 11:176:116907. PMID: 38865849
bioRxiv

2023 Jun 25.
bioRxiv

2023 Jan 17.
bioRxiv

January 25, 2022.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (188.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8811 mL

9.4057 mL

18.8115 mL

5 mM

0.3762 mL

1.8811 mL

3.7623 mL

10 mM

0.1881 mL

0.9406 mL

1.8811 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

For harringtonine treatment studies with Sindbis virus, BHK21 cells are seeded into 96-well plates and infected with Sindbis virus at an MOI of 1 for 1 h prior to being washed twice with PBS and incubated with various concentrations of harringtonine (0.1 μM, 1 μM, 5 μM, and 10 μM) at 37°C with 5% CO2. Cell supernatants are harvested for plaque assays at 24 h postinfection^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Fresno M, et al. Inhibition of translation in eukaryotic systems by harringtonine. Eur J Biochem. 1977 Jan;72(2):323-30. [Content Brief]

[2]. Kaur P, et al. Inhibition of chikungunya virus replication by harringtonine, a novel antiviral that suppresses viral protein expression. Antimicrob Agents Chemother. 2013 Jan;57(1):155-67. [Content Brief]

[3]. Piao YF, et al. Growth inhibition of human myeloid leukemia cells in vitro by harringtonine. Gan To Kagaku Ryoho. 1990 Feb;17(2):281-5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8811 mL	9.4057 mL	18.8115 mL	47.0287 mL
	5 mM	0.3762 mL	1.8811 mL	3.7623 mL	9.4057 mL
	10 mM	0.1881 mL	0.9406 mL	1.8811 mL	4.7029 mL
	15 mM	0.1254 mL	0.6270 mL	1.2541 mL	3.1352 mL
	20 mM	0.0941 mL	0.4703 mL	0.9406 mL	2.3514 mL
	25 mM	0.0752 mL	0.3762 mL	0.7525 mL	1.8811 mL
	30 mM	0.0627 mL	0.3135 mL	0.6270 mL	1.5676 mL
	40 mM	0.0470 mL	0.2351 mL	0.4703 mL	1.1757 mL
	50 mM	0.0376 mL	0.1881 mL	0.3762 mL	0.9406 mL
	60 mM	0.0314 mL	0.1568 mL	0.3135 mL	0.7838 mL
	80 mM	0.0235 mL	0.1176 mL	0.2351 mL	0.5879 mL
	100 mM	0.0188 mL	0.0941 mL	0.1881 mL	0.4703 mL

Keywords

Harringtonine

18 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Harringtonine

Anti-Infection Compound Library

Natural Product Library

FDA-Approved Drug Library

Anti-Cancer Compound Library

Antiviral Compound Library

Drug Repurposing Compound Library

NMPA-Approved Drug Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Alkaloids Library

Anti-Blood Cancer Compound Library

Anti-Cancer Natural Product Library

Antiviral Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

FDA-Approved Anticancer Drug Library

Off-patent Drug Library

FDA-Approved Traditional Chinese Medicine Active Compound Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Anti-Cancer Approved Drug Library

Mongolian Medicine Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Harringtonine

Biological Activity

Chemical Information

Publications (18)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Harringtonine Related Classifications

HY-N0862 Related Classifications