HEPES
Based on 10 publication(s) in Google Scholar
HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 7365-45-9
- Formula: C8H18N2O4S
- Molecular Weight:238.30
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) HEPES
More- Cancer Res. 2026 Feb 9. [Abstract]
- Neuron. 2025 Jul 29:S0896-6273(25)00509-4. [Abstract]
- J Adv Res. 2024 Aug 17:S2090-1232(24)00363-1. [Abstract]
- Chin Med. 2025 Jun 16;20(1):89. [Abstract]
- Biochem Pharmacol. 2026 Mar 22:249:117925. [Abstract]
- Int Immunopharmacol. 2023 Jul:120:110292. [Abstract]
- Int Immunopharmacol. 2022 Sep:110:108953. [Abstract]
- Structure. 2024 Dec 5;32(12):2287-2300.e4. [Abstract]
- Andrology. 2024 May 22. [Abstract]
- SSRN. 2026 May 6.
Biological Activity
HEPES maintains superhydrophilicity of titanium for at least 3 months and resulted in a continuous retention of bioactivity and osteoconductivity[1].
HEPES drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, resulting the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7365-45-9
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Appearance Solid
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Molecular Weight 238.30
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Formula C8H18N2O4S
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Color White to off-white
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SMILES
O=S(CCN1CCN(CCO)CC1)(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Publications (10)
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Journal Impact Factor
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Most Recent
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Cancer Res
The Farnesyl Transferase Inhibitor Darlifarnib (KO-2806) Resensitizes Relapsing Tumors to RAS Inhibition. [Abstract]2026 Feb 9. PMID: 41661652 -
Neuron
Social interaction in mice suppresses breast cancer progression via a corticoamygdala neural circuit. [Abstract]2025 Jul 29:S0896-6273(25)00509-4. PMID: 40752486 -
J Adv Res
Matrix stiffness-dependent PD-L2 deficiency improves SMYD3/xCT-mediated ferroptosis and the efficacy of anti-PD-1 in HCC. [Abstract]2024 Aug 17:S2090-1232(24)00363-1. PMID: 39159723 -
Chin Med
Quercetagetin alleviates liver fibrosis in non-alcoholic fatty liver disease by promoting ferroptosis of hepatic stellate cells through GPX4 ubiquitination. [Abstract]2025 Jun 16;20(1):89. PMID: 40524220 -
Biochem Pharmacol
Recruitment of tumor-associated macrophages via the CCL4-CCR5 axis promotes immune escape through PD-1 signaling in lung adenocarcinoma. [Abstract]2026 Mar 22:249:117925. PMID: 41876018 -
Int Immunopharmacol
2023 Jul:120:110292. PMID: 37182452 -
Int Immunopharmacol
Neobavaisoflavone inhibits allergic inflammatory responses by suppressing mast cell activation. [Abstract]2022 Sep:110:108953. PMID: 35724607 -
Structure
2024 Dec 5;32(12):2287-2300.e4. PMID: 39426373 -
Andrology
Cellular mechanism underlying leptin-induced anion secretion of rat epididymal epithelial cells. [Abstract]2024 May 22. PMID: 38778669 -
Solvent & Solubility
H2O : 200 mg/mL (839.28 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (271 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Suzuki T, et al. Nonvolatile buffer coating of titanium to prevent its biological aging and for drug delivery. Biomaterials. 2010;31(18):4818-4828. [Content Brief]
[2]. Sledź P, et al. An experimental charge density of HEPES. Acta Crystallogr B. 2010;66(Pt 4):482-492. [Content Brief]
[3]. Tol MJ, et al. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution. Autophagy. 2018;14(3):437-449. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.1964 mL | 20.9820 mL | 41.9639 mL | 104.9098 mL |
| 5 mM | 0.8393 mL | 4.1964 mL | 8.3928 mL | 20.9820 mL | |
| 10 mM | 0.4196 mL | 2.0982 mL | 4.1964 mL | 10.4910 mL | |
| 15 mM | 0.2798 mL | 1.3988 mL | 2.7976 mL | 6.9940 mL | |
| 20 mM | 0.2098 mL | 1.0491 mL | 2.0982 mL | 5.2455 mL | |
| 25 mM | 0.1679 mL | 0.8393 mL | 1.6786 mL | 4.1964 mL | |
| 30 mM | 0.1399 mL | 0.6994 mL | 1.3988 mL | 3.4970 mL | |
| 40 mM | 0.1049 mL | 0.5245 mL | 1.0491 mL | 2.6227 mL | |
| 50 mM | 0.0839 mL | 0.4196 mL | 0.8393 mL | 2.0982 mL | |
| 60 mM | 0.0699 mL | 0.3497 mL | 0.6994 mL | 1.7485 mL | |
| 80 mM | 0.0525 mL | 0.2623 mL | 0.5245 mL | 1.3114 mL | |
| 100 mM | 0.0420 mL | 0.2098 mL | 0.4196 mL | 1.0491 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
HEPES Related Classifications
HY-D0857 Related Classifications
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