USP30-IN-1
Based on 1 Customer Validation
USP30-IN-1 is a potent and selective USP30 inhibitor, with IC50 values of 94 nM and 4 nM in vivo and in vitro, respectively, and Ki values of 0.33 μM (Krippendorf method) and 0.38 μM (traditional method), respectively. USP30-IN-1 covalently binds to the catalytic cysteine (Cys77) of USP30, blocks ubiquitin substrate binding to inhibit deubiquitination, and ultimately induces mitophagy. USP30-IN-1 can be used for the research of idiopathic Parkinson's disease and mitophagy-related diseases.
For research use only. We do not sell to patients.
- Purity : 99.41%
- CAS No.: 2511541-78-7
- Formula: C17H15N5O2
- Molecular Weight:321.33
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
USP30 94 nM (IC50, in vivo) |
USP30 4 nM (IC50, in vitro) |
In Vitro
USP30-IN-1 (compound USP30-I-1) (0.01-10 μM; 1 h) blocks the binding of the HA-Ub-PA probe to endogenous USP30 in lysates of SH-SY5Y neuroblastoma cells, potently and specifically inhibits USP30 activity, but weakly inhibits USP10 activity[1].
USP30-IN-1 (30 min) dose-dependently inhibits the activity of USP30 in catalyzing the cleavage of ubiquitin-rhodamine substrate in a cell-free system[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:SH-SY5Y neuroblastoma cell lysates
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Concentration:0, 0.01, 0.1, 1, 10 μM
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Incubation Time:1 h
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Result:Dose-dependently inhibited the labeling of endogenous USP30 by HA-Ub-PA, demonstrating highly selective and potent target inhibition.
Chemical Information
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CAS No. 2511541-78-7
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Appearance Solid
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Molecular Weight 321.33
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Formula C17H15N5O2
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Color White to off-white
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SMILES
N#CC1=CC=CC(C2=CN=C(C(N[C@@H]3C[C@@H](C)N(C#N)C3)=O)O2)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (311.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Human pluripotent stem cell midbrain dopaminergic neuron differentiation
Human pluripotent stem cells are directed toward midbrain dopaminergic neurons by first inducing a neural floor-plate-like progenitor state, then patterning cells with ventralizing SHH signaling and midbrain/WNT-FGF cues, and finally maturing progenitors into neurons expressing dopaminergic markers such as TH, NURR1/NR4A2, PITX3, DAT/SLC6A3, VMAT2/SLC18A2, GIRK2/KCNJ6, FOXA2, LMX1A, and EN1. The main readouts are loss of pluripotency, acquisition of FOXA2+/LMX1A+ midbrain floor-plate progenitors, emergence of βIII-tubulin+/MAP2+ neurons, and production of TH+ dopaminergic neurons with molecular, dopamine-release, and electrophysiological features of midbrain dopaminergic identity.
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Mitophagy Solutions
Mitophagy is the selective autophagic degradation of mitochondria and functions as a mitochondrial quality-control pathway that removes damaged, depolarized, excess, or developmentally programmed mitochondria. The pathway links mitochondrial damage recognition, autophagosome recruitment, lysosomal delivery, and mitochondrial turnover to phenotypes such as mitochondrial homeostasis, oxidative-stress control, metabolic remodeling, differentiation, and neurodegeneration-related mitochondrial fidelity. The best-characterized damage-induced pathway is the PINK1-Parkin axis. Parkin is recruited selectively to impaired mitochondria and promotes their autophagic elimination, while mitochondrial depolarization stabilizes PINK1 on damaged mitochondria, recruits Parkin, and activates Parkin-dependent mitophagy. PINK1 also phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity, and PINK1-driven ubiquitin phosphorylation creates a feed-forward signal for recruiting autophagy machi
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1121 mL | 15.5603 mL | 31.1207 mL | 77.8016 mL |
| 5 mM | 0.6224 mL | 3.1121 mL | 6.2241 mL | 15.5603 mL | |
| 10 mM | 0.3112 mL | 1.5560 mL | 3.1121 mL | 7.7802 mL | |
| 15 mM | 0.2075 mL | 1.0374 mL | 2.0747 mL | 5.1868 mL | |
| 20 mM | 0.1556 mL | 0.7780 mL | 1.5560 mL | 3.8901 mL | |
| 25 mM | 0.1245 mL | 0.6224 mL | 1.2448 mL | 3.1121 mL | |
| 30 mM | 0.1037 mL | 0.5187 mL | 1.0374 mL | 2.5934 mL | |
| 40 mM | 0.0778 mL | 0.3890 mL | 0.7780 mL | 1.9450 mL | |
| 50 mM | 0.0622 mL | 0.3112 mL | 0.6224 mL | 1.5560 mL | |
| 60 mM | 0.0519 mL | 0.2593 mL | 0.5187 mL | 1.2967 mL | |
| 80 mM | 0.0389 mL | 0.1945 mL | 0.3890 mL | 0.9725 mL | |
| 100 mM | 0.0311 mL | 0.1556 mL | 0.3112 mL | 0.7780 mL |
USP30-IN-1 Related Classifications
HY-161909 Related Classifications
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