VAMP
Based on 1 Customer Validation
VAMP, a tetrapeptide, is a competitive dipeptidyl peptidase IV (DPP-IV) inhibitor with an IC50 of 1.00 μM and a Kd of 6.89 μM. VAMP effectively targets the DPP-IV-GLP-1 axis and can be used for the study of type 2 diabetes.
For research use only. We do not sell to patients.
- Purity : 98.13%
- CAS No.: 3103992-61-3
- Formula: C18H32N4O5S
- Molecular Weight:416.54
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
DPP-4 1 μM (IC50) |
DPP-4 6.89 μM (Kd) |
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 3103992-61-3
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Appearance Solid
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Molecular Weight 416.54
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Formula C18H32N4O5S
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Color White to off-white
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Sequence
Val-Ala-Met-Pro
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Sequence Shortening
VAMP
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (240.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Research Protocol for Metabolic Diseases
AMP-activated protein kinase, AMPK, is a conserved cellular energy sensor that responds to reduced cellular energy status and coordinates metabolism by increasing ATP-generating catabolic pathways while suppressing ATP-consuming anabolic processes. In metabolic disease research, the AMPK pathway is experimentally relevant because it regulates hepatic lipid synthesis, fatty acid oxidation, glucose production, skeletal-muscle glucose disposal, mTORC1-linked biosynthesis, autophagy, mitochondrial homeostasis, and whole-body energy balance. The central pathway logic is that energy stress, metformin, exercise-like stimulation, or direct AMPK activators increase AMPKα Thr172 phosphorylation and downstream substrate phosphorylation, including ACC and RAPTOR. Phosphorylation of ACC suppresses lipogenesis and supports fatty acid oxidation, whereas phosphorylation of RAPTOR suppresses mTORC1 signaling and links cellular energy status to growth and protein synthesis control. The pathway is linked
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4007 mL | 12.0036 mL | 24.0073 mL | 60.0182 mL |
| 5 mM | 0.4801 mL | 2.4007 mL | 4.8015 mL | 12.0036 mL | |
| 10 mM | 0.2401 mL | 1.2004 mL | 2.4007 mL | 6.0018 mL | |
| 15 mM | 0.1600 mL | 0.8002 mL | 1.6005 mL | 4.0012 mL | |
| 20 mM | 0.1200 mL | 0.6002 mL | 1.2004 mL | 3.0009 mL | |
| 25 mM | 0.0960 mL | 0.4801 mL | 0.9603 mL | 2.4007 mL | |
| 30 mM | 0.0800 mL | 0.4001 mL | 0.8002 mL | 2.0006 mL | |
| 40 mM | 0.0600 mL | 0.3001 mL | 0.6002 mL | 1.5005 mL | |
| 50 mM | 0.0480 mL | 0.2401 mL | 0.4801 mL | 1.2004 mL | |
| 60 mM | 0.0400 mL | 0.2001 mL | 0.4001 mL | 1.0003 mL | |
| 80 mM | 0.0300 mL | 0.1500 mL | 0.3001 mL | 0.7502 mL | |
| 100 mM | 0.0240 mL | 0.1200 mL | 0.2401 mL | 0.6002 mL |
VAMP Related Classifications
HY-P10809 Related Classifications
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