WH-4-023  (Synonyms: Dual LCK/SRC inhibitor)

WH-4-023 GMP is WH-4-023 (HY-12299) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. WH-4-023 (Dual LCK/SRC inhibitor) is a Lck/Src dual target inhibitor with functions in stem cell maintenance and differentiation regulation. WH-4-023 blocks epithelial-mesenchymal transition, supports the self-renewal of porcine embryonic stem cells, and inhibits their differentiation into mesoderm and endoderm. WH-4-023 is a key component of 3i/LAF medium, and enables the stable establishment and long-term maintenance of porcine pre-gastrulation epiblast stem cell lines. Removal of WH-4-023 reduces the expression of pluripotency factors in porcine and human extended pluripotent stem cells. WH-4-023 can be applied to relevant studies such as non-small cell lung cancer resistant to EGFR-TKIs^[1][2][3][4].

WH-4-023 (GMP) (>30 passages) supports long-term undifferentiated maintenance of doxycycline-independent porcine induced pluripotent stem cells (pEPSC^iPS) when included in pEPSCM, with cells retaining pluripotency marker expression matching porcine blastocysts through >30 passages^[1].
WH-4-023 (GMP) is required to maintain pluripotency factor expression in porcine and human expanded potential stem cells, though it supports lower pluripotency gene expression in hEPSCs than the alternative SRC inhibitor A419259 (HY-15764)^[1].
WH-4-023 (GMP) (48 h) inhibits ERRα protein expression and suppresses activation of the Src/Erk signaling cascade in PC-9, PC-9/GR, H1975, and PC-9/OR human NSCLC cell lines^[2].
WH-4-023 (GMP) (48 h) inhibits SP1 nuclear translocation in PC-9, PC-9/GR, H1975, and PC-9/OR human NSCLC cell lines^[2].
WH-4-023 (GMP) downregulates ERRα mRNA expression in PC-9, PC-9/GR, H1975, and PC-9/OR human NSCLC cell lines^[2].
WH-4-023 (GMP) (0.3 μM; 50+ passages) enables establishment and long-term maintenance of pESC-FIW cells (derived from in vitro porcine blastocysts) with enhanced proliferation, single-cell clonal efficiency, and stable pluripotency, while preventing EMT-associated spontaneous differentiation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

568.67

C₃₂H₃₆N₆O₄

837422-57-8

O=C(OC1=C(C)C=CC=C1C)N(C2=CC=C(OC)C=C2OC)C3=NC(NC4=CC=C(N5CCN(C)CC5)C=C4)=NC=C3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Gao X, et al. Establishment of porcine and human expanded potential stem cells. Nat Cell Biol. 2019;21(6):687-699.

  [2]. Pan Z, et al. Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression. Mol Cancer. 2022;21(1):77. Published 2022 Mar 18.  [Content Brief]

  [3]. Choi H, et al. Establishment of porcine embryonic stem cells in simplified serum free media and feeder free expansion. Stem Cell Res Ther. 2024;15(1):245. Published 2024 Aug 7.

  [4]. Zhi M, et al. Generation and characterization of stable pig pregastrulation epiblast stem cell lines. Cell Res. 2022;32(4):383-400.