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  4. WRR139

WRR139 is a peptide vinyl sulfone involving in disease processes such as inflammation and cancer. WRR139 is also a cytosolic enzyme N-glycanase 1 (NGLY1) and Nrf1 inhibitor. WRR139 enhances Carfilzomib cytotoxicity against cancer cells.

For research use only. We do not sell to patients.

WRR139 Chemical Structure

WRR139 Chemical Structure

CAS No. : 2138924-36-2

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5 mg USD 160 In-stock
10 mg USD 260 In-stock
25 mg USD 560 In-stock
50 mg USD 920 In-stock
100 mg USD 1480 In-stock
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Based on 1 publication(s) in Google Scholar

WRR139 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

WRR139 is a peptide vinyl sulfone involving in disease processes such as inflammation and cancer. WRR139 is also a cytosolic enzyme N-glycanase 1 (NGLY1) and Nrf1 inhibitor. WRR139 enhances Carfilzomib cytotoxicity against cancer cells[1].

In Vitro

WRR139 (0.01-100 μM; 1 h) inhibits NGLY1 in vitro and impairs processing of Nrf1 in HEK293 cells[1].
WRR139 (5 μM; 18 h) disrupts Nrf1 processing, location, and activation in HEK293 cells[1].
WRR139 (1 μM; 24 h) enhances the cytotoxicity of Carfilzomib in an NGLY1-dependent manner in U266, H929, and Jurkat cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

WRR139 Related Antibodies
Molecular Weight

456.60

Formula

C25H32N2O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCC(C)C)[C@@H](NC(/C=C/S(C1=CC=C(C)C=C1)(=O)=O)=O)CCC2=CC=CC=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (219.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1901 mL 10.9505 mL 21.9010 mL
5 mM 0.4380 mL 2.1901 mL 4.3802 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.71%

COA (253 KB) HNMR (289 KB) RP-HPLC (365 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1901 mL 10.9505 mL 21.9010 mL 54.7525 mL
5 mM 0.4380 mL 2.1901 mL 4.3802 mL 10.9505 mL
10 mM 0.2190 mL 1.0951 mL 2.1901 mL 5.4753 mL
15 mM 0.1460 mL 0.7300 mL 1.4601 mL 3.6502 mL
20 mM 0.1095 mL 0.5475 mL 1.0951 mL 2.7376 mL
25 mM 0.0876 mL 0.4380 mL 0.8760 mL 2.1901 mL
30 mM 0.0730 mL 0.3650 mL 0.7300 mL 1.8251 mL
40 mM 0.0548 mL 0.2738 mL 0.5475 mL 1.3688 mL
50 mM 0.0438 mL 0.2190 mL 0.4380 mL 1.0951 mL
60 mM 0.0365 mL 0.1825 mL 0.3650 mL 0.9125 mL
80 mM 0.0274 mL 0.1369 mL 0.2738 mL 0.6844 mL
100 mM 0.0219 mL 0.1095 mL 0.2190 mL 0.5475 mL
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WRR139 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WRR1392138924-36-2WRR 139WRR-139OthersInhibitorinhibitorinhibit

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