Xanthosine-5'-triphosphate
Xanthosine-5'-triphosphate (5'-XTP) is a purine nucleotide that stimulates Adenylate Cyclase to induce Cyclic AMP (cAMP) (HY-B1511) biosynthesis, binds to the AL1 allosteric site of the D137N-SAMHD1 mutant to activate dNTP triphosphohydrolase activity, and stimulates P2Y/P2U purinergic receptor-coupled phosphoinositide-specific phospholipase C with Michaelis-Menten kinetics. Xanthosine-5'-triphosphate can be used for HIV-1 research.
For research use only. We do not sell to patients.
- CAS No.: 6253-56-1
- Formula: C10H15N4O15P3
- Molecular Weight:524.17
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Sf9 | IC50 |
139 μM
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Inhibition of (-)-isoproterenol-stimulated GTP hydrolysis in Sf9 cell membranes expressing the β2-adrenergic receptor fused to the long splice variant of Gsα (β2ARGsαL) incubated for 20 min at 25°C by charcoal slurry-based GTPase assay.
Inhibition of (-)-isoproterenol-stimulated GTP hydrolysis in Sf9 cell membranes expressing the β2-adrenergic receptor fused to the long splice variant of Gsα (β2ARGsαL) incubated for 20 min at 25°C by charcoal slurry-based GTPase assay.
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39632506 |
In Vitro
Xanthosine-5'-Triphosphate (Various concentrations; 30 min) stimulates phosphoinositide phospholipase C in the ampullary epithelium of the posterior vertical semicircular canal of Rana ridibunda with a pKa of 3.59, an nH of 1.03, and an intrinsic activity of 0.9 relative to 1 mM ATP[3].
Xanthosine-5'-Triphosphate is identified as a substrate of inosine triphosphate pyrophosphatase protein in the 2DE-CETSA dataset of rat hippocampal cytosolic fraction[5].
Xanthosine-5'-Triphosphate (100 µM; 20 min) minimally increases basal adenylyl cyclase activity in Sf9 membranes expressing β2ARGsαL, but supports agonist-stimulated adenylyl cyclase activity with lower potency than GTP and ITP[6].
Xanthosine-5'-Triphosphate's hydrolysis (20 min) in Sf9 membranes is caused by endogenous nucleotidases, Gsα does not hydrolyze XTP to a measurable extent, and XTP hydrolysis is not regulated by β2AR ligands[6].
Xanthosine-5'-Triphosphate (139 µM; 20 min) blocks (-)-ISO-stimulated GTP hydrolysis in Sf9 membranes expressing β2ARGsαL, indicating that XTP binds to Gsα but is not hydrolyzed[6].
Xanthosine-5'-Triphosphate (5'-XTP) (10 min) is a potent agonist of phospholipase C in 1321N1 human astrocytoma cells expressing the avian tp2y receptor, with an EC50 of 253 nM[7].
Xanthosine-5'-Triphosphate (10 min) is a potent agonist that inhibits adenylyl cyclase in 1321N1 human astrocytoma cells expressing the avian tp2y receptor, with an EC50 of 172 nM[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (adult male, 250-350 g, halothane/methoxyflurane-nitrous oxide anaesthesia)[4]
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Dosage:0.2 M
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Administration:iontophoresis
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Result:Depressed 12 of 50 neurons tested.
Excited 11 of 33 quiescent or slowly firing neurons tested.
Chemical Information
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CAS No. 6253-56-1
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Molecular Weight 524.17
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Formula C10H15N4O15P3
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SMILES
O=C(C(N=C1)=C(N2)N1[C@@H]3O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)NC2=O
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Synonyms
5'-XTP
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Xanthosine-5'-triphosphate Related Classifications
HY-115736 Related Classifications
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