1-Adamantaneethanol
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1-Adamantaneethanol is an allele-selective MHC II class molecule loading enhancer (MHC loading enhancer, MLE) that enhances peptide loading and ligand exchange of HLA-DR molecules. 1-Adamantaneethanol targets the polymorphic P1 pocket of HLA-DR molecules and preferentially acts on HLA-DR subtypes with Gly at position β86, thereby stabilizing the peptide-receptor conformation and accelerating antigen loading. 1-Adamantaneethanol can be used in studies related to MHC II antigen presentation, peptide vaccines, adaptive immunity and tumor immunity.
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- Pureté : 99.82%
- CAS No.: 6240-11-5
- Formule: C12H20O
- Masse moléculaire:180.29
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
MHC II |
In Vitro
1-Adamantaneethanol (62.5-1000 μM; soluble DRB10101) accelerates HA306-318 peptide loading in a concentration-dependent manner; at 1 mM, it reduces the time required to reach half-maximal peptide loading from >20 h without a catalyst to approximately 30 min, and the enhancement of peptide loading rate remains unsaturated even at the highest concentration of 1 mM[1].
1-Adamantaneethanol (4 h) selectively enhances cell surface peptide loading of HLA-DR subtypes with Gly86, including DRB10101, DRB10401, DRB11502, DRB11602 and DRB50101, whereas Val86-containing DRB11501 is insensitive to AdEtOH; the Gly86→Val mutation abolishes the response, while the Val86→Gly mutation confers responsiveness to DRB11501[1].
1-Adamantaneethanol (50-250 μM; 4 h; DRB10101-expressing 721.221 cells) enhances HA306-318-specific CD4+ T cell responses in a concentration-dependent manner[1].
1-Adamantaneethanol (250 μM; 4 h) enhances CD4+ T-cell responses mediated by DRB10101-, DRB10401- and DRB11502-restricted antigen presentation, but does not enhance DRB11501-restricted responses, further indicating that its immunopotentiating effect is HLA-DR allele-selective[1].
1-Adamantaneethanol (250 μM; 4 h; bone marrow-derived dendritic cells from HLA-DR4 transgenic mice) enhances cell surface loading of HA306-318, increasing the streptavidin fluorescence MFI from 430 in the peptide alone group to 1607, and augments HLA-DR1/DR4-restricted HA306-318-specific CD4+ T cell responses[2].
1-Adamantaneethanol (250 μM; 4 h; HLA-DR-transfected L929 cells) enhances cell surface loading of the tumor-associated antigen NY-ESO-1 epitopes, with a significant enhancement in the loading of NY-ESO-1 89-101 onto HLA-DR1 and a weaker enhancement in the loading of NY-ESO-1 119-143 onto HLA-DR4[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Co-immunization with 1-Adamantaneethanol (2 μmol/mouse; s.c.; HLA-DR1 transgenic mice), HA306-318, IFA and CpG significantly increases the number of HA-specific IFN-γ-secreting T cells, while no significant enhancement is observed in BALB/c mice, indicating that its in vivo immunopotentiating effect is HLA-DR selective[2].
Combined immunization of HLA-DR1 transgenic mice with 1-Adamantaneethanol (2 μmol/mouse; s.c.) and 5 μg NY-ESO-1 89-101 increases the proportion of mice responding to this tumor-associated antigen from 4/13 to 12/13, and significantly elevates the number of IFN-γ-secreting cells[2].
Co-immunization with 1-Adamantaneethanol (2 μmol/mouse; s.c.; HLA-DR1 transgenic mice) and 10 μg recombinant NY-ESO-1 protein increases the proportion of NY-ESO-1-specific CD4+CD154+IFN-γ+ T cells from 0.064% to 0.23% on day 12, and elevates the sensitivity of NY-ESO-1-specific immune responses by nearly one order of magnitude[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HLA-DR1 transgenic mice; NY-ESO-1 89-101 priming[2]
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Dosage:2 μmol/mouse
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Administration:s.c.; single priming
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Result:Increased responders from 4/13 to 12/13 and significantly increased IFN-γ-secreting cells.
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Animal Model:HLA-DR4 transgenic mice; NY-ESO-1 119-143 priming[2]
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Dosage:2 μmol/mouse
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Administration:s.c.; single priming
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Result:Significantly enhanced antigen-specific T-cell priming.
Chemical Information
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CAS No. 6240-11-5
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Appearance Solid
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Masse moléculaire 180.29
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Formule C12H20O
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Color White to off-white
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SMILES
OCCC12C[C@H]3C[C@@H](C2)C[C@@H](C1)C3
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
Methanol : ≥ 100 mg/mL (554.66 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureté et documentation
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Fiche technique (286 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Methanol | 1 mM | 5.5466 mL | 27.7331 mL | 55.4662 mL | 138.6655 mL |
| 5 mM | 1.1093 mL | 5.5466 mL | 11.0932 mL | 27.7331 mL | |
| 10 mM | 0.5547 mL | 2.7733 mL | 5.5466 mL | 13.8665 mL | |
| 15 mM | 0.3698 mL | 1.8489 mL | 3.6977 mL | 9.2444 mL | |
| 20 mM | 0.2773 mL | 1.3867 mL | 2.7733 mL | 6.9333 mL | |
| 25 mM | 0.2219 mL | 1.1093 mL | 2.2186 mL | 5.5466 mL | |
| 30 mM | 0.1849 mL | 0.9244 mL | 1.8489 mL | 4.6222 mL | |
| 40 mM | 0.1387 mL | 0.6933 mL | 1.3867 mL | 3.4666 mL | |
| 50 mM | 0.1109 mL | 0.5547 mL | 1.1093 mL | 2.7733 mL | |
| 60 mM | 0.0924 mL | 0.4622 mL | 0.9244 mL | 2.3111 mL | |
| 80 mM | 0.0693 mL | 0.3467 mL | 0.6933 mL | 1.7333 mL | |
| 100 mM | 0.0555 mL | 0.2773 mL | 0.5547 mL | 1.3867 mL |
1-Adamantaneethanol Related Classifications
HY-112026 Related Classifications
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