2-Chloroisonicotinohydrazide
Based on 1 Customer Validation
2-Chloroisonicotinohydrazide (2-Chloro-isonicotinic acid hydrazide) (Compound 13a) is an Antibacterial agent. 2-Chloroisonicotinohydrazide exhibits antibacterial activity against Mycobacterium tuberculosis strain H37Rv, with a MIC50 of 392.0 μM. 2-Chloroisonicotinohydrazide can be used in the research of tuberculosis.
For research use only. We do not sell to patients.
- CAS No.: 58481-04-2
- Formula: C6H6ClN3O
- Molecular Weight:171.58
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
2-Chloroisonicotinohydrazide (392 μM) inhibits the growth of Mycobacterium tuberculosis strain H37Rv with an MIC of 392 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 58481-04-2
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Appearance Solid
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Molecular Weight 171.58
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Formula C6H6ClN3O
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SMILES
O=C(NN)C=1C=CN=C(Cl)C1
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Synonyms
2-Chloro-isonicotinic acid hydrazide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (582.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.8282 mL | 29.1409 mL | 58.2819 mL | 145.7046 mL |
| 5 mM | 1.1656 mL | 5.8282 mL | 11.6564 mL | 29.1409 mL | |
| 10 mM | 0.5828 mL | 2.9141 mL | 5.8282 mL | 14.5705 mL | |
| 15 mM | 0.3885 mL | 1.9427 mL | 3.8855 mL | 9.7136 mL | |
| 20 mM | 0.2914 mL | 1.4570 mL | 2.9141 mL | 7.2852 mL | |
| 25 mM | 0.2331 mL | 1.1656 mL | 2.3313 mL | 5.8282 mL | |
| 30 mM | 0.1943 mL | 0.9714 mL | 1.9427 mL | 4.8568 mL | |
| 40 mM | 0.1457 mL | 0.7285 mL | 1.4570 mL | 3.6426 mL | |
| 50 mM | 0.1166 mL | 0.5828 mL | 1.1656 mL | 2.9141 mL | |
| 60 mM | 0.0971 mL | 0.4857 mL | 0.9714 mL | 2.4284 mL | |
| 80 mM | 0.0729 mL | 0.3643 mL | 0.7285 mL | 1.8213 mL | |
| 100 mM | 0.0583 mL | 0.2914 mL | 0.5828 mL | 1.4570 mL |
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