24-Methylenecholesterol
Based on 2 publication(s) in Google Scholar
24-Methylenecholesterol (Ostreasterol) is a regulator targeting acyl-CoA cholesterol acyltransferase (ACAT) with anti-aging and neuroprotective effects. 24-Methylenecholesterol mimics the effects of nerve growth factor (NGF), can extend yeast lifespan through an anti-oxidative stress mechanism, and exhibits neuroprotective activity in PC12 cells. 24-Methylenecholesterol can reduce intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, activate anti-oxidative stress pathways (such as UTH1, SOD-related genes), and promote synaptic growth.
For research use only. We do not sell to patients.
- Purity : 99.78%
- CAS No.: 474-63-5
- Formula: C28H46O
- Molecular Weight:398.66
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-N1459
CampesterolEndogenous Metabolite -
HY-N0322
Cholesterol (from animal)ERRα Agonist -
HY-N0171A
Beta-Sitosterol (purity>98%)Dietary Plant Sterols -
HY-N0131
StigmasterolPlant Sterol -
HY-113224
DesmosterolEndogenous Metabolite -
HY-N7255
CycloartenolApoptosis Inducer -
HY-130703
5-DehydroepisterolC28 Alkylated Sterol -
HY-133968R
24-Methylenecholesterol (Standard)Anti-inflammatory Sterol -
HY-119738
OSMI-1O-GlcNAc Transferase Inhibitor -
HY-114361
OSMI-4OGT Inhibitor -
HY-N1422
HecogeninFungal Inhibitor -
HY-N1422R
Hecogenin (Standard)Fungal Inhibitor
Publications Citing Use of MedChemExpress (MCE) 24-Methylenecholesterol
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero | IC50 |
>128 μg/mL
Compound: 1
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Cytotoxicity against african green monkey Vero cells
Cytotoxicity against african green monkey Vero cells
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[PMID: 18818073] |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Apis mellifera worker bees (female, newly emerged, 80-100 mg body weight), nutritional physiology model[2]
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Dosage:0.1% (S1), 0.25% (S2), 0.5% (S3), 0.75% (S4), 1.0% (S5) 24-Methylenecholesterol dissolved in acetone and mixed into synthetic diets (final acetone concentration <0.5%)
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Administration:Oral administration via synthetic diet, replaced daily, treatment for 3 weeks starting from newly emerged adulthood
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Result:Treatment with 24-Methylenecholesterol at 0.1-1.0% dry diet concentration significantly altered the head proteome of worker bees.
Compared to the control group, the mean relative abundances of major royal jelly proteins (MRJP1, MRJP4, MRJP5, MRJP7)-key nutritional markers involved in brood food synthesis-were upregulated by 20-35% in the S3-S5 groups (0.5-1.0% sterol).
Chemical Information
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CAS No. 474-63-5
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Appearance Solid
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Molecular Weight 398.66
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Formula C28H46O
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Color White to off-white
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SMILES
C=C(C(C)C)CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Synonyms
Ostreasterol
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Structure Classification
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Initial Source
the larva of queen bees and from royal jelly
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Plant J
Unveiling the inequivalent biochemical functions of OsSMO2-1 and OsSMO2-2 in rice phytosterol biosynthesis using a customized sterol standards mixture. [Abstract]2025 Nov;124(3):e70559. PMID: 41194406 -
Biomed Rep
Chalinasterol attenuates ethanol-induced hepatic lipid accumulation by activating β-oxidation. [Abstract]2026 Mar 9;24(5):57. PMID: 41859047
Solvent & Solubility
In Vitro:
Ethanol : 16.67 mg/mL (41.82 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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PC12 NGF-Induced Neuronal Differentiation Culture
PC12 cells are a rat adrenal pheochromocytoma clonal line that responds to NGF by stopping proliferation and extending branching neurite-like processes; after longer NGF exposure, cells develop long processes and neuronal-like ultrastructural and functional features. NGF-induced differentiation is read out mainly by neurite outgrowth, reduced proliferation, microtubule assembly, and neuronal differentiation-associated proteins such as MAPs, tau, GAP-43, and synapsin-1.
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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PC12 NGF-induced neuronal-like differentiation
PC12 cells are a rat adrenal pheochromocytoma-derived clonal cell line that responds to nerve growth factor by stopping proliferation and extending neurites, producing a sympathetic neuron-like phenotype used to study neuronal differentiation and neurite outgrowth. NGF acts through TrkA-dependent signaling, and neurite outgrowth is associated with ERK/Akt signaling, microtubule organization, neuronal-marker expression, and increased electrophysiological neuronal features such as sodium-channel density. The main assay readout is morphological differentiation, usually measured as the percentage of neurite-bearing cells, neurite length, neurite number, or total neurite length per cell. Additional readouts include GAP-43, tyrosine hydroxylase, βIII-tubulin, neurofilament, synapsin I, synaptophysin, ERK phosphorylation, Akt phosphorylation, and sodium-channel current density.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Sun Y, et al. Sterols from Mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity. Int J Mol Sci. 2014 Nov 25;15(12):21660-73. [Content Brief]
[2]. Chakrabarti P, et al. Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol. Insects. 2020 Oct 29;11(11):743. [Content Brief]
[3]. Tabas I, et al. The reactivity of desmosterol and other shellfish- and xanthomatosis-associated sterols in the macrophage sterol esterification reaction. J Clin Invest. 1989 Dec;84(6):1713-21. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.5084 mL | 12.5420 mL | 25.0840 mL | 62.7101 mL |
| 5 mM | 0.5017 mL | 2.5084 mL | 5.0168 mL | 12.5420 mL | |
| 10 mM | 0.2508 mL | 1.2542 mL | 2.5084 mL | 6.2710 mL | |
| 15 mM | 0.1672 mL | 0.8361 mL | 1.6723 mL | 4.1807 mL | |
| 20 mM | 0.1254 mL | 0.6271 mL | 1.2542 mL | 3.1355 mL | |
| 25 mM | 0.1003 mL | 0.5017 mL | 1.0034 mL | 2.5084 mL | |
| 30 mM | 0.0836 mL | 0.4181 mL | 0.8361 mL | 2.0903 mL | |
| 40 mM | 0.0627 mL | 0.3136 mL | 0.6271 mL | 1.5678 mL |
24-Methylenecholesterol Related Classifications
HY-133968 Related Classifications
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