2. Protein Tyrosine Kinase/RTK Metabolic Enzyme/Protease
  3. Bcr-Abl Liposome
  4. 3'-BPANeuGc

3'-BPANeuGc is a high-affinity selective Siglec-G ligand. Lipid nanoparticles surface-modified with antigens and 3'-BPANeuGc inhibit BCR signal transduction. 3'-BPANeuGc is applicable to antigen-specific tolerance research.

For research use only. We do not sell to patients.

3'-BPANeuGc

3'-BPANeuGc Chemical Structure

CAS No. : 1596134-81-4

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3'-BPANeuGc Related Antibodies

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Description

3'-BPANeuGc is a high-affinity selective Siglec-G ligand. Lipid nanoparticles surface-modified with antigens and 3'-BPANeuGc inhibit BCR signal transduction. 3'-BPANeuGc is applicable to antigen-specific tolerance research[1].

In Vitro

3'-BPANeuGc (1-4%; 40 min at 4°C) is a selective, high-affinity ligand for Siglec-G, as demonstrated by specific binding to Siglec-G-expressing BW5147 cells and murine splenic B cells, with no cross-reactivity to other murine siglecs[1].
3'-BPANeuGc (1-4%) when co-displayed with anti-IgM on liposomes substantially suppresses calcium flux in both murine peritoneal B1a cells and splenic B2 cells in a Siglec-G-dependent manner[1].
3'-BPANeuGc (1-4%; 3 min) when co-displayed with HEL on liposomes-mediated recruitment of Siglec-G to the BCR strongly dampens proximal and distal BCR signaling cascade components in splenic HEL-reactive MD4 B cells in an Shp-1-dependent manner[1].
3'-BPANeuGc (1-4%; 4 days) when co-displayed with HEL on liposomes inhibits proliferation and reduces survival of HEL-reactive MD4 B cells in a Siglec-G-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3'-BPANeuGc Related Antibodies

Western Blot Analysis[1]

Cell Line: splenic HEL-reactive MD4 B cells (wild-type and Shp-1-deficient)
Concentration: 1-4% (in 5 μM liposomes containing HEL)
Incubation Time: 3 min
Result: Suppressed phosphorylation or degradation of multiple BCR signaling components, including those involved in MAP kinase, Erk, NFkB, and Akt pathways, when co-displayed with HEL on liposomes, compared to liposomes displaying HEL alone.
Showed no inhibitory effect in Shp-1-deficient (Moth eaten) B cells.

Cell Proliferation Assay[1]

Cell Line: HEL-reactive MD4 B cells (wild-type and Siglec-G-deficient)
Concentration: 1-4% (in liposomes containing HEL)
Incubation Time: 4 days
Result: Caused pronounced diminishment in proliferation and a significant reduction in live cell numbers when co-displayed with HEL on liposomes, compared to liposomes displaying HEL alone.
Showed no such effects in Siglec-G-deficient MD4 B cells.
In Vivo

Liposomes displaying 3'-BPANeuGc (0.5 mM total lipid concentration; i.p., i.v.; single dose) and the T-independent antigen NP induce robust antigen-specific B cell tolerance in mice, as evidenced by reduced antibody titers and lack of response to subsequent antigen challenge[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (wild-type; Siglec-G-deficient; MD4 transgenic, IgMHEL-expressing)[1]
Dosage: 0.5 mM total lipid concentration (delivered in 200 µl PBS)
Administration: i.p.; single dose; i.v.; single dose
Result: Showed greatly reduced anti-NP IgM and IgG titers compared to mice receiving NP-only liposomes.
Failed to produce anti-NP antibodies upon subsequent challenge with NP-only liposomes.
Molecular Weight

926.91

Formula

C41H58N4O20
CAS No.
SMILES

OC([C@@]1(O[C@@]([C@@H]([C@H](C1)O)NC(CO)=O)([H])[C@H](O)[C@H](O)CNC(CC2=CC=C(C3=CC=CC=C3)C=C2)=O)O[C@@H]4[C@H]([C@@H](O[C@@H]([C@@H]4O)CO)O[C@@H]5[C@H](O[C@H]([C@@H]([C@H]5O)NC(C)=O)OCCN)CO)O)=O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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3'-BPANeuGc Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3'-BPANeuGc1596134-81-4Bcr-AblLiposomeBW5147 cellsmurine splenic B cellsmurine peritoneal B1a cellsSiglec-GB1 B cellsCD22B2 B cellsB cell receptorMD4 B cellsSHP-1Inhibitorinhibitorinhibit

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