3'-O-(2-Nitrobenzyl)-dTTP
3'-O-(2-Nitrobenzyl)-dTTP acts as a reversible terminator for DNA chain elongation. 3'-O-(2-Nitrobenzyl)-dTTP interacts with 9°N polymerase (exo-) A485L/Y409V, gets recognized and incorporated into the elongating DNA chain, thereby temporarily terminating the polymerase reaction. 3'-O-(2-Nitrobenzyl)-dTTP carries a 3'-O-(2-nitrobenzyl) blocking group, which can be removed by laser irradiation to regenerate the free 3'-OH− group and restore polymerase activity. 3'-O-(2-Nitrobenzyl)-dTTP is applicable in DNA sequencing studies.
For research use only. We do not sell to patients.
- CAS No.: 219607-49-5
- Formula: C17H22N3O16P3
- Molecular Weight:617.29
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
3'-O-(2-Nitrobenzyl)-dTTP (100 pmol) is efficiently and quantitatively incorporated as a substrate by 9°N polymerase (exo-)A485L/Y409V in solution-phase polymerase extension reactions, generating a single extension product detectable at 5602 m/z by MALDI-TOF MS[1].
3'-O-(2-Nitrobenzyl)-dTTP (200 pmol) functions as an effective reversible terminator for pyrosequencing, allowing accurate, unambiguous deciphering of homopolymeric T regions in Sepharose bead-immobilized DNA templates via discrete, per-nucleotide PPi signal detection[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 219607-49-5
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Molecular Weight 617.29
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Formula C17H22N3O16P3
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SMILES
OP(OC[C@H]1O[C@@H](N2C(NC(C(C)=C2)=O)=O)C[C@@H]1OCC3=CC=CC=C3[N+]([O-])=O)(OP(OP(O)(O)=O)(O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
3'-O-(2-Nitrobenzyl)-dTTP Related Classifications
HY-185274 Related Classifications
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