3-sucCA
Based on 1 Customer Validation
3-sucCA is an orally available bacterial bile acid that exerts anti-MASH effects by promoting the growth of Akkermansia muciniphila. By remodeling the intestinal microbiota and promoting the growth of Akkermansia, 3-sucCA can improve intestinal barrier damage and reduce chronic low-level inflammation, thereby alleviating the progression of metabolic dysfunction-associated steatohepatitis (MASH). 3-sucCA accelerates the synthesis of cell wall peptidoglycan and has in vivo efficacy in the mouse MAFL-MASH model. 3-sucCA levels are low in the MAFLD model and are mainly used in the study of MASH.
For research use only. We do not sell to patients.
- Purity : 99.92%
- CAS No.: 94159-47-4
- Formula: C28H44O8
- Molecular Weight:508.64
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
3-sucCA (1 mM, 10 mM, 50 mM; 48 h) promotes the growth of Akkermansia muciniphila in a dose-dependent manner[1].
3-sucCA (20 μM; 3 days) is relatively stable when incubated with feces-derived in vitro communities, while glycocholic acid was degraded within 24 h[1].
3-sucCA (10 μM; 48 h) significantly promotes the growth of Akkermansia muciniphila and inhibited the growth of Clostridium sporogenes and Enterococcus hirae[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:97 different bacterial species
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Concentration:10 μM
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Incubation Time:48 hours
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Result:Significantly increased the growth yield of Akkermansia muciniphila and showed inhibitory effects on Clostridium sporogenes and Enterococcus hirae.
In Vivo
3-sucCA (10 mg/kg; oral gavage; 3 times a week; 3 weeks) fails to improve liver injury and liver histological abnormalities in the MASH model of germ-free (GF) C57BL/6J mice induced by CDAA-HFD diet[1].
3-sucCA (10 mg/kg; oral gavage; 3 times a week; 3 weeks) significantly attenuates the alleviating effect of MASH in the MASH model of Tlr4-/- C57BL/10ScNJ mice induced by CDAA-HFD diet[1].
3-sucCA (10 mg/kg; oral gavage; single dose) has a bioavailability of 11.5% in the SPF C57BL/6J mouse model and is mainly distributed in the colon contents[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SPF C57BL/6J mice (male, 8-week-old) with CDAA-HFD-induced MASH model[1]
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Dosage:10 mg/kg
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Administration:oral gavage, 3 times per week, 3 weeks
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Result:Reduced the ratio of liver mass to body mass, plasma levels of ALT and AST, and hepatic triglyceride content.
Alleviated hepatic steatosis, inflammation, and fibrosis, as shown by H&E, oil red O, and Sirius red staining.
The relative mRNA expression of genes involved in hepatic lipid synthesis, pro-inflammatory cytokine production, and collagen synthesis was decreased.
Chemical Information
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CAS No. 94159-47-4
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Appearance Solid
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Molecular Weight 508.64
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Formula C28H44O8
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Color White to off-white
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SMILES
O[C@H]1[C@@]2([H])[C@@]3([H])[C@@]([C@@](CC3)([H])[C@H](C)CCC(O)=O)([C@H](C[C@]2([H])[C@@]4([C@](C[C@@H](CC4)OC(CCC(O)=O)=O)([H])C1)C)O)C
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Synonyms
3-Succinylated cholic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 175 mg/mL (344.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Research Protocol for Microbiome Analysis
Microbiome analysis characterizes microbial communities in biological or environmental samples by measuring community composition, diversity, taxonomic structure, functional potential, and associations with host or environmental phenotypes. 16S rRNA gene amplicon sequencing is commonly used for bacterial and archaeal taxonomic profiling, while shotgun metagenomics provides higher taxonomic resolution and direct functional information, including microbial genes, pathways, viruses, fungi, and antimicrobial-resistance genes when sequencing depth and host-DNA contamination are adequately controlled. Microbiome results are strongly affected by sample collection, storage, DNA extraction, contamination, sequencing method, reference database, and bioinformatic pipeline; therefore, standardized protocols, negative controls, mock communities, and transparent analysis workflows are required. Unresolved issues include low-biomass contamination, compositional-data bias, inconsistent species-level c
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Nie Q, et al. Gut symbionts alleviate MASH through a secondary bile acid biosynthetic pathway. Cell. 2024 May 23;187(11):2717-2734.e33. [Content Brief]
[2]. Chang S, et al. Succinate supplementation alleviates liver cancer by inhibiting the FN1/SQLE axis-mediated cholesterol biosynthesis. iScience. 2025 Jan 2;28(2):111731. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9660 mL | 9.8301 mL | 19.6603 mL | 49.1507 mL |
| 5 mM | 0.3932 mL | 1.9660 mL | 3.9321 mL | 9.8301 mL | |
| 10 mM | 0.1966 mL | 0.9830 mL | 1.9660 mL | 4.9151 mL | |
| 15 mM | 0.1311 mL | 0.6553 mL | 1.3107 mL | 3.2767 mL | |
| 20 mM | 0.0983 mL | 0.4915 mL | 0.9830 mL | 2.4575 mL | |
| 25 mM | 0.0786 mL | 0.3932 mL | 0.7864 mL | 1.9660 mL | |
| 30 mM | 0.0655 mL | 0.3277 mL | 0.6553 mL | 1.6384 mL | |
| 40 mM | 0.0492 mL | 0.2458 mL | 0.4915 mL | 1.2288 mL | |
| 50 mM | 0.0393 mL | 0.1966 mL | 0.3932 mL | 0.9830 mL | |
| 60 mM | 0.0328 mL | 0.1638 mL | 0.3277 mL | 0.8192 mL | |
| 80 mM | 0.0246 mL | 0.1229 mL | 0.2458 mL | 0.6144 mL | |
| 100 mM | 0.0197 mL | 0.0983 mL | 0.1966 mL | 0.4915 mL |
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