2. 4-Aminobenzohydrazide

4-Aminobenzohydrazide  (Synonyms: p-Aminobenzohydrazide; p-Aminobenzoic acid hydrazide)

Cat. No.: HY-B0880 Purity: 99.96%
COA Handling Instructions

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4-Aminobenzohydrazide is an irreversible MPO myeloperoxidase inhibitor with an IC50 of 0.3 µM. It is used for the research of subacute stroke.

For research use only. We do not sell to patients.

4-Aminobenzohydrazide Chemical Structure

4-Aminobenzohydrazide Chemical Structure

CAS No. : 5351-17-7

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Based on 1 publication(s) in Google Scholar

4-Aminobenzohydrazide Related Antibodies

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1 Publications Citing Use of MCE 4-Aminobenzohydrazide

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Description

4-Aminobenzohydrazide is an irreversible MPO myeloperoxidase inhibitor with an IC50 of 0.3 µM[1]. It is used for the research of subacute stroke[2].

Molecular Weight

151.17

Formula

C7H9N3O
CAS No.
Appearance

Solid

Color

Light yellow to brown

SMILES

O=C(NN)C1=CC=C(N)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 25 mg/mL (165.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.6151 mL 33.0753 mL 66.1507 mL
5 mM 1.3230 mL 6.6151 mL 13.2301 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (6.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1 mg/mL (6.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.96%

COA (244 KB) HNMR (113 KB) LCMS (249 KB) GC (183 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.6151 mL 33.0753 mL 66.1507 mL 165.3767 mL
5 mM 1.3230 mL 6.6151 mL 13.2301 mL 33.0753 mL
10 mM 0.6615 mL 3.3075 mL 6.6151 mL 16.5377 mL
15 mM 0.4410 mL 2.2050 mL 4.4100 mL 11.0251 mL
20 mM 0.3308 mL 1.6538 mL 3.3075 mL 8.2688 mL
25 mM 0.2646 mL 1.3230 mL 2.6460 mL 6.6151 mL
30 mM 0.2205 mL 1.1025 mL 2.2050 mL 5.5126 mL
40 mM 0.1654 mL 0.8269 mL 1.6538 mL 4.1344 mL
50 mM 0.1323 mL 0.6615 mL 1.3230 mL 3.3075 mL
60 mM 0.1103 mL 0.5513 mL 1.1025 mL 2.7563 mL
80 mM 0.0827 mL 0.4134 mL 0.8269 mL 2.0672 mL
100 mM 0.0662 mL 0.3308 mL 0.6615 mL 1.6538 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Aminobenzohydrazide5351-17-7p-Aminobenzohydrazide p-Aminobenzoic acid hydrazideInhibitorinhibitorinhibit

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