Δ4-Dafachronic acid

Cat. No.: HY-131492 Purity: 96.93%: Data Sheet
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Δ4‑Dafachronic acid is an endogenous steroid hormone, an agonist of the DAF‑12 nuclear receptor, and a key ligand for the vitamin D receptor (VDR). Δ4‑Dafachronic acid promotes the active escape developmental trajectory in Austrofundulus limnaeus embryos even under diapause‑inducing conditions. Δ4‑Dafachronic acid is suitable for research on the developmental biology.

For research use only. We do not sell to patients.

Δ4-Dafachronic acid Chemical Structure

CAS No. : 23017-97-2

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	Get quote
10 mg	Get quote

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Other Forms of Δ4-Dafachronic acid:

Δ4-Dafachronic Acid-d₃ Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Δ4-Dafachronic acid (0.3 mM) is the preferred substrate for the SDENChol_v1_11189 ACS enzyme^[2].
Δ4-dafachronic acid (2.3 μM; 3-6 days) promotes molting of Haemonchus contortus L₃ to L₄ in cell-free in vitro culture^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Δ4-dafachronic acid (25-75 μM; immersion in embryo media; daily media change) induces the escape (continuous development) trajectory in Austrofundulus limnaeus embryos under diapause-favoring 25°C conditions in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Austrofundulus limnaeus embryos^[1]
Dosage:	25 μM; 50 μM; 75 μM
Administration:	immersion in embryo media; daily media change
Result:	Induced ~15% of embryos to follow escape trajectory and ~85% to enter diapause at 25 μM. Induced ~40% of embryos to follow escape trajectory and ~60% to enter diapause at 50 μM. Induced ~90% of embryos to follow escape trajectory and ~10% to enter diapause at 75 μM. Produced escape embryos with developmental rate and pattern indistinguishable from normal escape embryos. Resulted in delayed development for embryos that entered diapause despite treatment.

Molecular Weight

414.62

Formula

C₂₇H₄₂O₃

CAS No.

23017-97-2

Appearance

Solid

Color

White to off-white

SMILES

CC(C(O)=O)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(CC[C@]4(C)[C@@]3([H])CC[C@]12C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 2.5 mg/mL (6.03 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4118 mL	12.0592 mL	24.1185 mL
5 mM	0.4824 mL	2.4118 mL	4.8237 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 96.93%

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Data Sheet (275 KB) SDS (252 KB)

COA (249 KB) HNMR (264 KB) RP-HPLC (95 KB) MS (156 KB)

Handling Instructions (2659 KB)

References

[1]. Romney ALT, et al. Temperature-dependent vitamin D signaling regulates developmental trajectory associated with diapause in an annual killifish. Proc Natl Acad Sci U S A. 2018;115(50):12763-12768. [Content Brief]

[2]. Warnke M, et al. Functional Characterization of Three Specific Acyl-Coenzyme A Synthetases Involved in Anaerobic Cholesterol Degradation in Sterolibacterium denitrificans Chol1S. Appl Environ Microbiol. 2018;84(7):e02721-17. Published 2018 Mar 19.

[3]. Niciura SCM, et al. In vitro culture of parasitic stages of Haemonchus contortus. Rev Bras Parasitol Vet. 2023;32(1):e010122. Published 2023 Jan 13.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4118 mL	12.0592 mL	24.1185 mL	60.2962 mL
DMSO	5 mM	0.4824 mL	2.4118 mL	4.8237 mL	12.0592 mL