5-Me-dC(Ac) amidite
Based on 1 Customer Validation
5-Me-dC(Ac) amidite is an acetyl (Ac)-modified nucleotide commonly used in the synthesis of modified oligonucleotides.
For research use only. We do not sell to patients.
- Purity: 97.0%
- CAS No.: 1873306-74-1
- Formula: C42H52N5O8P
- Molecular Weight:785.86
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
Oligonucleotides are a class of short-chain nucleic acids composed of deoxyribonucleotides or ribonucleotides; by adhering to the principle of complementary base pairing, they can pair with DNA, mRNA, or pre-mRNA to precisely "silence" specific genes, thereby inhibiting the expression of numerous proteins.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1873306-74-1
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Appearance Solid
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Molecular Weight 785.86
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Formula C42H52N5O8P
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Color White to off-white
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SMILES
COC(C=C1)=CC=C1C(C2=CC=CC=C2)(C3=CC=C(OC)C=C3)OC[C@@H]4[C@@H](OP(N(C(C)C)C(C)C)OCCC#N)C[C@H](N5C(N=C(NC(C)=O)C(C)=C5)=O)O4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (127.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2725 mL | 6.3625 mL | 12.7249 mL | 31.8123 mL |
| 5 mM | 0.2545 mL | 1.2725 mL | 2.5450 mL | 6.3625 mL | |
| 10 mM | 0.1272 mL | 0.6362 mL | 1.2725 mL | 3.1812 mL | |
| 15 mM | 0.0848 mL | 0.4242 mL | 0.8483 mL | 2.1208 mL | |
| 20 mM | 0.0636 mL | 0.3181 mL | 0.6362 mL | 1.5906 mL | |
| 25 mM | 0.0509 mL | 0.2545 mL | 0.5090 mL | 1.2725 mL | |
| 30 mM | 0.0424 mL | 0.2121 mL | 0.4242 mL | 1.0604 mL | |
| 40 mM | 0.0318 mL | 0.1591 mL | 0.3181 mL | 0.7953 mL | |
| 50 mM | 0.0254 mL | 0.1272 mL | 0.2545 mL | 0.6362 mL | |
| 60 mM | 0.0212 mL | 0.1060 mL | 0.2121 mL | 0.5302 mL | |
| 80 mM | 0.0159 mL | 0.0795 mL | 0.1591 mL | 0.3977 mL | |
| 100 mM | 0.0127 mL | 0.0636 mL | 0.1272 mL | 0.3181 mL |
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