8-Oxo-GTP  (Synonyms: 8-Hydroxyguanosine triphosphate)

8-Oxo-GTP is an oxidized guanine nucleotide, produced by reactive oxygen species (ROS). 8-Oxo-GTP serves as a substrate for MutT protein, GTP cyclohydrolase II, Mycobacterium tuberculosis MutT1, and Mycobacterium tuberculosis Rv1700, which hydrolyze it to corresponding nucleoside monophosphates or diphosphates. 8-Oxo-GTP causes detrimental effects including mutations and mistranslation when incorporated into nucleic acids. 8-Oxo-GTP can be used for the research of cancer and tuberculosis^[1][2][3].

8-Oxo-GTP (8-Hydroxyguanosine triphosphate) (15-100 μM; 30 min) is efficiently hydrolyzed to 8-Oxo-GMP by purified and histidine-tagged recombinant Escherichia coli GTP cyclohydrolase II with an apparent K_m of 21 μM and a K_cat of 4.0 min^-1, at a rate higher than that of GTP hydrolysis^[1].
8-Oxo-GTP (250 μM; 30 min) is converted to 8-oxo-GDP by purified MtuMutT1 at 37 °C^[2].
8-Oxo-GTP (250 μM; 30 min) is converted to 8-oxo-GDP and 8-oxo-GMP by purified MtuMutT1 and Rv1700 together at 37 °C^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

539.18

C₁₀H₁₆N₅O₁₅P₃

21238-36-8

O[C@H]1[C@@H](O)[C@H](N2C(N=C(N)NC3=O)=C3N=C2O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kobayashi M, et al. Potential of Escherichia coli GTP cyclohydrolase II for hydrolyzing 8-oxo-dGTP, a mutagenic substrate for DNA synthesis. J Biol Chem. 1998;273(41):26394-26399.

  [2]. Patil AG, et al. Mycobacterium tuberculosis MutT1 (Rv2985) and ADPRase (Rv1700) proteins constitute a two-stage mechanism of 8-oxo-dGTP and 8-oxo-GTP detoxification and adenosine to cytidine mutation avoidance. J Biol Chem. 2013;288(16):11252-11262.  [Content Brief]

  [3]. Kim HJ, et al. 8-oxo-7,8-dihydroguanosine triphosphate(8-oxoGTP) down-regulates respiratory burst of neutrophils by antagonizing GTP toward Rac, a small GTP binding protein. Free Radic Res. 2007;41(6):655-662.