1. Neuronal Signaling
  2. CaMK

Calmodulin-Dependent Protein Kinase II 290-309 

Cat. No.: HY-P1479
Handling Instructions

Calmodulin-Dependent Protein Kinase II (290-309) is a potent CaMK antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase II.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Calmodulin-Dependent Protein Kinase II 290-309 Chemical Structure

Calmodulin-Dependent Protein Kinase II 290-309 Chemical Structure

CAS No. : 115044-69-4

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Calmodulin-Dependent Protein Kinase II (290-309) is a potent CaMK antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase II.

IC50 & Target

IC50: 52 nM (calmodulin-dependent protein kinase II)[1]

In Vitro

Peptide 290 to 309 is found to be a potent calmodulin antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase. Neither truncation from the amino terminus (peptide 296-309) nor extension in the carboxyl-terminal direction (peptide 294-319) markedly affects calmodulin binding, whereas shortening the peptide from the carboxyl terminus (peptide 290-302) or from both ends (peptide 295-304) results in the elimination of this activity[1].

References
Kinase Assay
[1]

Ca2+/CaM-dependent phosphodiesterase is assayed at 30°C. The reaction mixture contains 40 mM Tris-HCl, pH 8.0, 5 mM magnesium acetate, 1 mM calcium chloride, 30 μM cGMP, 0.15 pCi of [3H]cGMP, 1 mM dithiothreitol, 20% glycerol, 0.64 mg/mL bovine serum albumin, 2.38 nM CaM, 50 pM CaM-deficient phosphodiesterase, and various concentrations of Calmodulin-Dependent Protein Kinase II (290-309). Following preincubation of the reaction mixture at 30°C, the reaction is initiated by the addition of substrate. After 50 min the reaction is terminated by boiling. Conditions are selected in which the reactions are linear with respect to time[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

2273.83

Formula

C₁₀₃H₁₈₅N₃₁O₂₄S

CAS No.

115044-69-4

Sequence

Leu-Lys-Lys-Phe-Asn-Ala-Arg-Arg-Lys-Leu-Lys-Gly-Ala-Ile-Leu-Thr-Thr-Met-Leu-Ala

Sequence Shortening

LKKFNARRKLKGAILTTMLA

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Calmodulin-Dependent Protein Kinase II 290-309
Cat. No.:
HY-P1479
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Calmodulin-Dependent Protein Kinase II 290-309

Cat. No.: HY-P1479