1. Membrane Transporter/Ion Channel
  2. Chloride Channel

Chlorotoxin 

Cat. No.: HY-P0173A Purity: >98.0%
Handling Instructions

Chlorotoxin is a 36 amino-acid peptide from the venom of the Israeli scorpion Leiurus quinquestriatus with anticancer activity. Chlorotoxin is a chloride channel blocker. Sequence: Met-Cys-Met-Pro-Cys-Phe-Thr-Thr-Asp-His-Gln-Met-Ala-Arg-Lys-Cys-Asp-Asp-Cys-Cys-Gly-Gly-Lys-Gly-Arg-Gly-Lys-Cys-Tyr-Gly-Pro-Gln-Cys-Leu-Cys-Arg-NH2(Disulfide bridge: Cys2-Cys19,Cys5-Cys28,,Cys16-Cys33,Cys20-Cys35).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Chlorotoxin Chemical Structure

Chlorotoxin Chemical Structure

CAS No. : 163515-35-3

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100 μg USD 180 Get quote
500 μg USD 450 Get quote
1 mg USD 660 Get quote

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Chlorotoxin is a 36 amino-acid peptide from the venom of the Israeli scorpion Leiurus quinquestriatus with anticancer activity. Chlorotoxin is a chloride channel blocker. Sequence: Met-Cys-Met-Pro-Cys-Phe-Thr-Thr-Asp-His-Gln-Met-Ala-Arg-Lys-Cys-Asp-Asp-Cys-Cys-Gly-Gly-Lys-Gly-Arg-Gly-Lys-Cys-Tyr-Gly-Pro-Gln-Cys-Leu-Cys-Arg-NH2(Disulfide bridge: Cys2-Cys19,Cys5-Cys28,,Cys16-Cys33,Cys20-Cys35).

IC50 & Target

Target: Chloride Channel[1]

In Vitro

Chlorotoxin (Chlorotoxin) preferentially binds to tumor cells and has been harnessed to develop an imaging agent to help visualize tumors during surgical resection. In addition, chlorotoxin has potential as a vehicle to deliver anti-cancer drugs specifically to cancer cells. Chlorotoxin is shown to bind glioma cells, but is unable to bind normal rat astrocytes and Te671, a human rhabdomyosarcoma cell line. Chlorotoxin inhibits the migration of U251MG (glioma) cells, with an IC50 of 600 nM[2]. Chlorotoxin binds to glioma cells is specific and involves high affinity (Kd=4.2 nM) and low affinity (Kd=660 nM) binding sites[3].Small conductance chloride channels are shown to be potently blocked by Chlorotoxin. Chlorotoxin has been used as a general pharmacological tool to investigate the function of chloride channels[4].

In Vivo

Chlorotoxin shows insecticidal activity on insects and other invertebrates. After the administration of I-Chlorotoxin to tumor-bearing mice, the peptides accumulated within the tumor[2].Chlorotoxin selectively accumulates in the brain of tumor-bearing mice with calculated brain: muscle ratios of 36.4% of injected dose/g (ID/g), as compared to 12.4%ID/g in control animals[3].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.2503 mL 1.2513 mL 2.5027 mL
5 mM 0.0501 mL 0.2503 mL 0.5005 mL
10 mM 0.0250 mL 0.1251 mL 0.2503 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[3]

Mouse: At 24, 48, 72, and 96 h after tumor-bearing and control SCID mice are injected with 125l-labeled Chlorotoxin, they are anesthetized and imaged. Both 125I- and 131l-labeled Chlorotoxin-injected animals and their control counterparts are killed at indicated time points for biodistribution studies[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

3995.71

Formula

C₁₅₈H₂₄₉N₅₃O₄₇S₁₁

CAS No.

163515-35-3

Storage
Pure form -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Chlorotoxin
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HY-P0173A
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Chlorotoxin

Cat. No.: HY-P0173A