1. Membrane Transporter/Ion Channel
  2. Potassium Channel
  3. Clofilium tosylate

Clofilium tosylate 

Cat. No.: HY-33350 Purity: 99.77%
Handling Instructions

Clofilium tosylate, a potassium channel blocker, induces apoptosis of human promyelocytic leukemia (HL-60) cells via Bcl-2-insensitive activation of caspase-3. Antiarrhythmic agent.

For research use only. We do not sell to patients.

Clofilium tosylate Chemical Structure

Clofilium tosylate Chemical Structure

CAS No. : 92953-10-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 110 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
50 mg USD 420 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Clofilium tosylate, a potassium channel blocker, induces apoptosis of human promyelocytic leukemia (HL-60) cells via Bcl-2-insensitive activation of caspase-3. Antiarrhythmic agent[1].

IC50 & Target

Potassium channel[1]

In Vitro

HL-60 cells treated with Clofilium (0-20 μM; 24, 48, and 72 hours) lead to suppression of viability and proliferation in both time and concentration-dependent manners. Cell viability decreases significantly in HL-60 cells treated with 2.5 μM to 10 μM of Clofilium[1].
Clofilium (10 μM, 12 hours) induces the proteolytic cleavage of inactive procaspase-3, p34 into its active form, p17 and subsequent cleavage of its substrate PARP at 2 h after exposure to 10 mM Clofilium. However, there is no significant change in expression of Bcl-2 and Bax proteins[1].

Cell Viability Assay[1]

Cell Line: HL-60 cells
Concentration: 0-20 μM
Incubation Time: 24, 48, and 72 hours
Result: Inhibited HL-60 cells with IC50s of 6.3 μM for 24 hours, 3.4 μM for 48 hours, 2.4 μM for 72 hours, respectively.

Western Blot Analysis[1]

Cell Line: HL-60 cells
Concentration: 10 μM
Incubation Time: 12 hours
Result: Induced proteolytic cleavage of caspase-3 and subsequent cleavage of its substrate, PARP, while Bcl-2 and Bax proteins were unaltered.
Storage

4°C, protect from light

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (245.02 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9601 mL 9.8007 mL 19.6013 mL
5 mM 0.3920 mL 1.9601 mL 3.9203 mL
10 mM 0.1960 mL 0.9801 mL 1.9601 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.08 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.08 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.08 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Molecular Weight

510.17

Formula

C₂₈H₄₄ClNO₃S

CAS No.

92953-10-1

SMILES

CCCCCCC[N+](CC)(CCCCC1=CC=C(Cl)C=C1)CC.O=S(C2=CC=C(C)C=C2)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Clofilium tosylate
Cat. No.:
HY-33350
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Clofilium tosylate

Cat. No.: HY-33350