Cat. No.: HY-N0936

Coixol

(Synonyms: 6-Methoxy-2-benzoxazolinone; 6-MBOA)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Coixol (6-Methoxy-2-benzoxazolinone; 6-MBOA) is a potent and orally active anti-inflammatory agent. Coixol decreases the iNOS protein expression. Coixol inhibits the production of TNF-α, IL-6, and IL-1β. Coixol improves glucose tolerance and plasma insulin. Coixol decreases the blood glucose level.

For research use only. We do not sell to patients.

Purity: 98.56%
CAS No.: 532-91-2
Formula: C₈H₇NO₃
Molecular Weight:165.15

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Coixol Chemical Structure

CAS No.: 532-91-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	>100 μM Compound: 1; 2a	Cytotoxicity against mouse RAW264.7 cells incubated for 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells incubated for 24 hrs by MTT assay	[PMID: 37561816]
RAW264.7	IC₅₀	31.2 μM Compound: 1; 2a	Inhibition of TNF-alpha production in LPS-induced mouse RAW264.7 cells pretreated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA Inhibition of TNF-alpha production in LPS-induced mouse RAW264.7 cells pretreated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA	[PMID: 37561816]
RAW264.7	IC₅₀	48.9 μM Compound: 1; 2a	Inhibition of IL-6 production in LPS-induced mouse RAW264.7 cells pretreated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA Inhibition of IL-6 production in LPS-induced mouse RAW264.7 cells pretreated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA	[PMID: 37561816]
RAW264.7	IC₅₀	66.4 μM Compound: 1; 2a	Inhibition of IL-1beta production in LPS-induced mouse RAW264.7 cells pretreated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA Inhibition of IL-1beta production in LPS-induced mouse RAW264.7 cells pretreated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA	[PMID: 37561816]

In Vitro

Coixol (5, 10, 20 µM, 2+24 h) decreases the iNOS protein expression in LPS-induced RAW264.7 Cells^[2].
Coixol (0.03, 0.1, 0.3, 1, 3, 10, 30, 100 μM; 1+24 h) inhibits the production of TNF-α, IL-6, and IL-1β with IC₅₀s of 31.2, 48.9, 66.4 µM, respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

RAW264.7 cells
Concentration:

5, 10, 20 µM
Incubation Time:

2 h and then stimulated with or without LPS (0.5 μg/mL) for 24 h
Result:

Decreased the iNOS protein expression in a dose dependent manner.

In Vivo

Coixol (25, 50 mg/kg, p.o.) improves glucose tolerance and stimulates glucose-induced plasma insulin in non-diabetic and diabetic rats^[3].
Coixol (25, 50 mg/kg, p.o.; daily for 15 day) decreases the blood glucose levels in a dose- and time-dependent manner in type 2 diabetic rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

9–12 weeks, Sprague Dawley (SD) male rats^[3]
Dosage:

25, 50 mg/kg
Administration:

P.o.
Result:

Decreased the blood glucose levels significantly, improved glucose tolerance.

Chemical Information

CAS No. 532-91-2
Appearance Solid
Molecular Weight 165.15
Formula C₈H₇NO₃
Color Light brown to brown
SMILES

O=C1OC2=CC(OC)=CC=C2N1
Synonyms

6-Methoxy-2-benzoxazolinone; 6-MBOA
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Marine natural products
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Journal Impact Factor

Planta Medica International Open

2022; 9(01): e108-e115.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (605.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.0551 mL

30.2755 mL

60.5510 mL

5 mM

1.2110 mL

6.0551 mL

12.1102 mL

10 mM

0.6055 mL

3.0276 mL

6.0551 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (15.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (15.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yusheng Hu, et al. Coixol Suppresses NF-κB, MAPK Pathways and NLRP3 Inflammasome Activation in Lipopolysaccharide-Induced RAW 264.7 Cells. Molecules [Content Brief]

[2]. Cui E, et al. Discovery of Coixol Derivatives as Potent Anti-inflammatory Agents. J Nat Prod. 2023 Aug 25;86(8):1950-1959. [Content Brief]

[3]. Hameed A, et al. Coixol amplifies glucose-stimulated insulin secretion via cAMP mediated signaling pathway. Eur J Pharmacol. 2019 Sep 5;858:172514. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.0551 mL	30.2755 mL	60.5510 mL	151.3775 mL
	5 mM	1.2110 mL	6.0551 mL	12.1102 mL	30.2755 mL
	10 mM	0.6055 mL	3.0276 mL	6.0551 mL	15.1378 mL
	15 mM	0.4037 mL	2.0184 mL	4.0367 mL	10.0918 mL
	20 mM	0.3028 mL	1.5138 mL	3.0276 mL	7.5689 mL
	25 mM	0.2422 mL	1.2110 mL	2.4220 mL	6.0551 mL
	30 mM	0.2018 mL	1.0092 mL	2.0184 mL	5.0459 mL
	40 mM	0.1514 mL	0.7569 mL	1.5138 mL	3.7844 mL
	50 mM	0.1211 mL	0.6055 mL	1.2110 mL	3.0276 mL
	60 mM	0.1009 mL	0.5046 mL	1.0092 mL	2.5230 mL
	80 mM	0.0757 mL	0.3784 mL	0.7569 mL	1.8922 mL
	100 mM	0.0606 mL	0.3028 mL	0.6055 mL	1.5138 mL

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Coixol

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Publications Citing Use of MedChemExpress (MCE) Coixol

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Coixol Related Classifications

HY-N0936 Related Classifications