1. Cell Cycle/DNA Damage
    Cytoskeleton
    Apoptosis
  2. Microtubule/Tubulin
    Apoptosis
  3. Estramustine phosphate sodium

Estramustine phosphate sodium 

Cat. No.: HY-13627 Purity: 99.42%
Handling Instructions

Estramustine phosphate sodium, an estradiol analog, is an orally active antimicrotubule chemotherapy agent. Estramustine phosphate sodium depolymerises microtubules by binding to microtubule associated proteins (MAPs) and/or to tubulin. Estramustine phosphate sodium can interfere mitosis, trigger cell death and induce apoptosis, which can be used for the research of cancer like prostate cancer.

For research use only. We do not sell to patients.

Estramustine phosphate sodium Chemical Structure

Estramustine phosphate sodium Chemical Structure

CAS No. : 52205-73-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in Water USD 79 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 79 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 72 In-stock
Estimated Time of Arrival: December 31
50 mg USD 119 In-stock
Estimated Time of Arrival: December 31
100 mg USD 168 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Other Forms of Estramustine phosphate sodium:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Estramustine phosphate sodium, an estradiol analog, is an orally active antimicrotubule chemotherapy agent. Estramustine phosphate sodium depolymerises microtubules by binding to microtubule associated proteins (MAPs) and/or to tubulin. Estramustine phosphate sodium can interfere mitosis, trigger cell death and induce apoptosis, which can be used for the research of cancer like prostate cancer[1][2][3].

In Vitro

Estramustine phosphate sodium (1 µg/ mL, 48 h) suppresses PC3 cell growth[1].
Estramustine phosphate sodium (2 µg/mL, 48 h) elevates phosphatidylserine eversion amount on PC3 cells and induces PC3 cell apoptosis through reducing miR-31[1].
Estramustine phosphate sodium (0-40 µM, 24-72 h) inhibits cell proliferation and tubulin cytoskeleton in RAW 264.7 cells[2].
Estramustine phosphate sodium (10 µM, 24 h) inhibits TGF-β-induced RAW 264.7 cell migration, as well as TGF-β-induced uPA production by inhibiting Smad3 activation[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: PC3 cells
Concentration: 1 µg/ mL
Incubation Time: 48 h
Result: Suppressed PC3 cell growth.

Immunofluorescence[2]

Cell Line: RAW 264.7 cells
Concentration: 10 µM
Incubation Time: 24 h
Result: Disrupted the interphase microtubules.

Cell Migration Assay [2]

Cell Line: 18 h of TGF-β treated RAW 264.7 cells
Concentration: 10 µM
Incubation Time: 24 h
Result: Inhibited TGF-β-Induced Cell Migration.
In Vivo

Estramustine phosphate sodium (Intraperitoneal injection, 4 or 12 mg/kg, a daily dose for 2 weeks) inhibits PAC120 tumor growth 53% by day 35[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss nu/nu (nude) male mice (5-week-old) bearing PAC120 tumors[3]
Dosage: 4 mg/kg, 12 mg/kg
Administration: Intraperitoneal injection; daily; for 2 weeks
Result: Suppressed the development of skin lesions and resulted in a dissociation between DTH response and antibody production.
Animal Model: Human prostate cancer xenograft PAC120[3]
Dosage: 4 or 12 mg/kg, a daily dose for 2 weeks.
Administration: Intraperitoneal injection
Result: Inhibited PAC120 tumor growth 53% by day 35.
Clinical Trial
Molecular Weight

564.35

Formula

C23H30Cl2NNa2O6P

CAS No.
SMILES

O=C(N(CCCl)CCCl)OC1=CC2=C(C=C1)[[email protected]@]3([H])CC[[email protected]@]4(C)[[email protected]](CC[[email protected]@H]4OP(O[Na])(O[Na])=O)([H])[[email protected]]3([H])CC2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (110.75 mM; Need ultrasonic)

DMSO : 5 mg/mL (8.86 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7720 mL 8.8598 mL 17.7195 mL
5 mM 0.3544 mL 1.7720 mL 3.5439 mL
10 mM 0.1772 mL 0.8860 mL 1.7720 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 65 mg/mL (115.18 mM); Clear solution; Need ultrasonic

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.42%

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Estramustine phosphate sodium
Cat. No.:
HY-13627
Quantity:
MCE Japan Authorized Agent: