Cat. No.: HY-B0535A

Ethambutol dihydrochloride

(Synonyms: Emb dihydrochloride)

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Ethambutol dihydrochloride is an antimycobacterial compound that prevents cell wall formation by inhibiting arabinosyltransferase activity. Ethambutol dihydrochloride can be used to establish animal models of hyperuricemia and optic neuropathy.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 1070-11-7
Formula: C₁₀H₂₆Cl₂N₂O₂
Molecular Weight:277.23

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Ethambutol dihydrochloride Chemical Structure

CAS No.: 1070-11-7

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Induced Disease Models
- Clinical Trial
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Antibiotic Isoforms :

Induced Disease Models

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Ethambutol dihydrochloride can be used in animal modeling to build a model of hyperuricemia.

1. Induction of Hyperuricaemic Disease^[4]^[5]

Background

Elevated uric acid levels can lead to crystal deposition and may cause gout, urolithiasis, and urate nephropathy. Ethambutol dihydrochloride can cause increased uric acid levels by reducing renal uric acid clearance, ultimately leading to hyperuricemia

Specific Modeling Methods

Rat^[4]: Male • Wistar rats • SPF • 200-220 g
Administration: 250 mg/kg (Ethambutol) • intragastric administration • once daily for 23 days

Note

(1) Ethambutol dihydrochloride-induced hyperuricemia model should be combined with adenine (HY-B0152) (100 mg/kg; gavage) to achieve better model construction effect^[4].
(2) Some compounds can synergistically promote uric acid production, such as: Ethanol and Vitamin B12 (HY-B0315)^[5].
(3) Ethambutol dihydrochloride-induced hyperuricemia model can be controlled by xanthine oxidase inhibitors.

Modeling Indicators

Molecular changes: URAT1 level in kidney homogenate ↑, OAT1 level ↓.

Physiological changes: Serum uric acid level ↑; sparse cells, tissue cell number ↑, lymphocyte number ↓.

Pathological changes: Pathological damage and inflammatory response in the thymus and spleen; thymus atrophy, cortical medullary fusion; splenic sinus congestion, with iron-yellow deposits.

Opposite Product(s): Xanthine Oxidase Inhibitors

2. Induction of Optic Neuropathy^[6]

Background

Ethambutol dihydrochloride treatment can cause binocular toxicity at certain doses and durations. There is currently no clear treatment to prevent damage to retinal neuronal cells in cases of Ethhambutol dihydrochloride-induced optic neuropathy (EON).

Specific Modeling Methods

Rat: Wistar rats
Administration: 35 mg/kg/day (Ethambutol) • po • 30 days

Modeling Indicators

Molecular changes: Bcl-2, Caspase-3 expression levels ↑.

Pathological indicators: Ganglion cell damage, increased density of retinal ganglion cells (RGCs); thickening of the RGC cell layer due to vacuoles formed in the ganglion cell cytoplasm.

Opposite Product(s): Citicoline (HY-B0739)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 1070-11-7
Appearance Solid
Molecular Weight 277.23
Formula C₁₀H₂₆Cl₂N₂O₂
Color White to off-white
SMILES

CC[C@H](NCCN[C@@H](CC)CO)CO.Cl.Cl
Synonyms

Emb dihydrochloride
Structure Classification
- Others
Initial Source
- Microorganisms
Mycobacterium smegmatis
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (4)

All

Sort by:

Journal Impact Factor

mLife

Identification of a phenyl ester covalent inhibitor of caseinolytic protease and analysis of the ClpP1P2 inhibition in mycobacteria. [Abstract]

2025 Apr 15;4(2):155-168. PMID: 40313980
Commun Biol

The acetyltransferase CysE modulates virulence and drug resistance of Mycobacterium tuberculosis by interfering with oxidative stress responses. [Abstract]

2025 Oct 6;8(1):1425. PMID: 41053365
Antimicrob Agents Chemother

Incorporation of macrophage immune stresses into an intracellular assay of drug tolerance in Mycobacterium tuberculosis. [Abstract]

2025 Sep 11:e0079525. PMID: 40934365
ACS Chem Biol

Investigate Natural Product Indolmycin and the Synthetically Improved Analogue Toward Antimycobacterial Agents. [Abstract]

2022 Jan 21;17(1):39-53. PMID: 34908399

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (360.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (180.36 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6071 mL

18.0356 mL

36.0711 mL

5 mM

0.7214 mL

3.6071 mL

7.2142 mL

10 mM

0.3607 mL

1.8036 mL

3.6071 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (360.71 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ethambutol. Tuberculosis (Edinb), 2008. 88(2): p. 102-5. [Content Brief]

[2]. Rastogi N, et al. In vitro activities of fourteen antimicrobial agents against drug susceptible and resistant clinical isolates of Mycobacterium tuberculosis and comparative intracellular activities against the virulent H37Rv strain in human macrophages. Curr Microbiol, 1996. 33(3): p. 167-75. [Content Brief]

[3]. Kaur D, et al. In vitro, ex-vivo and in vivo activities of ethambutol and sparfloxacin alone and in combination against mycobacteria. Int J Antimicrob Agents, 2001. 17(1): p. 51-5. [Content Brief]

[4]. Kang L, et al. Total glucosides of herbaceous peony (Paeonia lactiflora Pall.) flower attenuate adenine- and ethambutol-induced hyperuricaemia in rats. J Ethnopharmacol. 2020 Oct 28;261:113054. [Content Brief]

[5]. Pham AQ, et al. Pyrazinamide-induced hyperuricemia. P T. 2014 Oct;39(10):695-715. [Content Brief]

[6]. Nusanti S, et al. The Effect of Citicoline on Ethambutol Optic Neuropathy: Histopathology and Immunohistochemistry Analysis of Retina Ganglion Cell Damage Level in Rat Model. J Ocul Pharmacol Ther. 2022 Oct;38(8):584-589. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.6071 mL	18.0356 mL	36.0711 mL	90.1778 mL
	5 mM	0.7214 mL	3.6071 mL	7.2142 mL	18.0356 mL
	10 mM	0.3607 mL	1.8036 mL	3.6071 mL	9.0178 mL
	15 mM	0.2405 mL	1.2024 mL	2.4047 mL	6.0119 mL
	20 mM	0.1804 mL	0.9018 mL	1.8036 mL	4.5089 mL
	25 mM	0.1443 mL	0.7214 mL	1.4428 mL	3.6071 mL
	30 mM	0.1202 mL	0.6012 mL	1.2024 mL	3.0059 mL
	40 mM	0.0902 mL	0.4509 mL	0.9018 mL	2.2544 mL
	50 mM	0.0721 mL	0.3607 mL	0.7214 mL	1.8036 mL
	60 mM	0.0601 mL	0.3006 mL	0.6012 mL	1.5030 mL
	80 mM	0.0451 mL	0.2254 mL	0.4509 mL	1.1272 mL
	100 mM	0.0361 mL	0.1804 mL	0.3607 mL	0.9018 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Ethambutol dihydrochloride

4 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Ethambutol dihydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

Natural Product Library

FDA-Approved Drug Library

Drug Repurposing Compound Library

Antibacterial Compound Library

NMPA-Approved Drug Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Microbial Metabolite Library

Rare Diseases Drug Library

Pediatric Drug Library

Human Metabolite Library

Anti-Pulmonary Fibrosis Compound Library

Off-patent Drug Library

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Natural Product and Natural Product-Like Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Ethambutol dihydrochloride

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Ethambutol dihydrochloride Related Classifications

HY-B0535A Related Classifications