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GJ103 sodium salt 

Cat. No.: HY-101203A Purity: 98.67%
COA Handling Instructions

GJ103 sodium salt is an active analog of the read-through compound GJ072.

For research use only. We do not sell to patients.

GJ103 sodium salt Chemical Structure

GJ103 sodium salt Chemical Structure

CAS No. : 1459687-96-7

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Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 88 In-stock
Estimated Time of Arrival: December 31
Solid
2 mg USD 55 In-stock
Estimated Time of Arrival: December 31
5 mg USD 110 In-stock
Estimated Time of Arrival: December 31
10 mg USD 176 In-stock
Estimated Time of Arrival: December 31
25 mg USD 352 In-stock
Estimated Time of Arrival: December 31
50 mg USD 605 In-stock
Estimated Time of Arrival: December 31
100 mg USD 825 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

GJ103 sodium salt is an active analog of the read-through compound GJ072.

In Vitro

Chemical-induced read through of premature stop codons might be exploited as a potential treatment strategy for genetic disorders caused by nonsense mutations. GJ072 is a novel read-through compound (RTC). GJ072 shows activity comparable to stop codons (TGA, TAG, and TAA) PTC124 and RTC13. GJ072 induces ATM kinase on both TGA and TAG stop codons and restored ATMpSer1981 autophosphorylation and SMC1pSer966 transphosphorylation as measured by FACS. GJ072 is active in A-T cells with a homozygous TAA mutation. GJ072 is able to induce detectable full-length ATM protein in treated A-T cells. Early structure-activity relationship studies generates eight active analogs of GJ072. Some GJ072 analogs (e.g., GJ103, GJ106, GJ109, and GJ111) consistently demonstrates their activities in all three PTCs by both FCATMpSer1981 and IRIF assays. GJ071 and GJ072 and some of their analogs (such as GJ103) have similar read-through activity as RTC13 or RTC14, but are more tolerable than RTC13 and RTC14 to A-T cells. GJ103 does not show obvious cytotoxicity in A-T cells at concentration as high as 300 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

GJ103 sodium salt is water soluble, making it much easier to work with in in vivo experiments[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

364.35

Formula

C16H13N4NaO3S

CAS No.
SMILES

O=C(O[Na])CSC1=NN=C(C2=NC=CC=C2)N1C3=CC=CC(OC)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (137.23 mM; Need ultrasonic)

H2O : ≥ 10 mg/mL (27.45 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7446 mL 13.7231 mL 27.4461 mL
5 mM 0.5489 mL 2.7446 mL 5.4892 mL
10 mM 0.2745 mL 1.3723 mL 2.7446 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
Cell Assay
[1]

Cytotoxicity is measured by cell proliferation assay. Cells are seeded into a flat-bottom 96-well plate, including control wells containing complete growth medium alone as blank absorbance readings. After RTC treatment (GJ103), activated-XTT Solution is added into each well, and the cells are returned to the cell culture incubator for 12-14 hours. The absorbance is measured at 480 nM with relevant 630 nM to assess nonspecific readings[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GJ103 sodium salt
Cat. No.:
HY-101203A
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