VH032-PEG5-C6-Cl (Synonyms: HaloPROTAC 2)

Name: VH032-PEG5-C6-Cl
Brand: MedChemExpress
SKU: HY-112495
Rating: 4.5 (1 reviews)

Cat. No.: HY-112495 Purity: 99.76%: FAQs
Data Sheet SDS COA Handling Instructions

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VH032-PEG5-C6-Cl (HaloPROTAC 2) is a conjugate of ligands for E3 and 21-atom-length linker. The connector of linker is Halogen group. VH032-PEG5-C6-Cl incorporates the VH032 based VHL ligand and 5-unit PEG linker. VH032-PEG5-C6-Cl is capable of inducing the degradation of GFP-HaloTag7 in cell-based assays.

For research use only. We do not sell to patients.

VH032-PEG5-C6-Cl Chemical Structure

CAS No. : 1799506-06-1

Size	Price	Stock	Quantity
5 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 640	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1280	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2050	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3250	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

VHL

In Vitro

Treatment (24 hour) with VH032-PEG5-C6-Cl leads to nearly 70% degradation of GFP-Halotag7 at 2.5 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

783.41

Formula

C₃₈H₅₉ClN₄O₉S

CAS No.

1799506-06-1

Appearance

Viscous Liquid

SMILES

O=C(NCC1=CC=C(C2=C(C)N=CS2)C=C1)[C@H]3N(C([C@H](C(C)(C)C)NC(COCCOCCOCCOCCOCCCCCCCl)=O)=O)C[C@H](O)C3

Shipping

Shipping with dry ice.

Storage

-80°C, protect from light, stored under nitrogen

Solvent & Solubility

In Vitro:

DMSO : 270 mg/mL (344.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 100 mg/mL (127.65 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2765 mL	6.3824 mL	12.7647 mL
5 mM	0.2553 mL	1.2765 mL	2.5529 mL
10 mM	0.1276 mL	0.6382 mL	1.2765 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (6.38 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (6.38 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (6.38 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (269 KB) SDS (251 KB)

COA (245 KB) HNMR (482 KB) LCMS (126 KB)

Handling Instructions (2659 KB)

References

[1]. Buckley DL, et al. HaloPROTACS: Use of Small Molecule PROTACs to Induce Degradation of HaloTag Fusion Proteins. ACS Chem Biol. 2015 Aug 21;10(8):1831-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	1.2765 mL	6.3824 mL	12.7647 mL	31.9118 mL
	5 mM	0.2553 mL	1.2765 mL	2.5529 mL	6.3824 mL
	10 mM	0.1276 mL	0.6382 mL	1.2765 mL	3.1912 mL
	15 mM	0.0851 mL	0.4255 mL	0.8510 mL	2.1275 mL
	20 mM	0.0638 mL	0.3191 mL	0.6382 mL	1.5956 mL
	25 mM	0.0511 mL	0.2553 mL	0.5106 mL	1.2765 mL
	30 mM	0.0425 mL	0.2127 mL	0.4255 mL	1.0637 mL
	40 mM	0.0319 mL	0.1596 mL	0.3191 mL	0.7978 mL
	50 mM	0.0255 mL	0.1276 mL	0.2553 mL	0.6382 mL
	60 mM	0.0213 mL	0.1064 mL	0.2127 mL	0.5319 mL
	80 mM	0.0160 mL	0.0798 mL	0.1596 mL	0.3989 mL
	100 mM	0.0128 mL	0.0638 mL	0.1276 mL	0.3191 mL

VH032-PEG5-C6-Cl Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VH032-PEG5-C6-Cl1799506-06-1HaloPROTAC 2HaloPROTAC2HaloPROTAC-2E3 Ligase Ligand-Linker ConjugatesInhibitorinhibitorinhibit

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VH032-PEG5-C6-Cl (Synonyms: HaloPROTAC 2)

Customer Review

VH032-PEG5-C6-Cl Related Antibodies

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•Related Small Molecules:

•Related Proteins:

View All E3 Ligase Ligand-Linker Conjugates Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

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Complete Stock Solution Preparation Table

VH032-PEG5-C6-Cl Related Classifications

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