1. Membrane Transporter/Ion Channel
    Apoptosis
  2. Potassium Channel
    Apoptosis
  3. NS-1619

NS-1619 

Cat. No.: HY-12496 Purity: ≥98.0%
Handling Instructions

NS-1619 is an opener of large conductance Ca2+-activated K+ (BK) channel. NS-1619 is a highly effective relaxant with an EC50 of about 10 – 30 μM in several smooth muscles of blood vessels and other tissues. NS1619 inhibits proliferation and induces apoptosis in A2780 ovarian cancer cells.

For research use only. We do not sell to patients.

NS-1619 Chemical Structure

NS-1619 Chemical Structure

CAS No. : 153587-01-0

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Solution
10 mM * 1 mL in DMSO USD 90 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
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Estimated Time of Arrival: December 31
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5 mg USD 82 In-stock
Estimated Time of Arrival: December 31
10 mg USD 120 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE NS-1619

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Description

NS-1619 is an opener of large conductance Ca2+-activated K+ (BK) channel. NS-1619 is a highly effective relaxant with an EC50 of about 10 – 30 μM in several smooth muscles of blood vessels and other tissues[1]. NS1619 inhibits proliferation and induces apoptosis in A2780 ovarian cancer cells[2].

In Vitro

NS1619 (5, 10, 30, 50, and 100 μM) inhibits the proliferation of A2780 cells in a dosage and time dependent manner IC50=31.1 μM for 48 h pretreatment[2].
NS1619 (30 μM) exhibits augmenting effect on whole cell IK in human ovarian cancer cells A2780[2].
NS1619 (10, 30, 50, and 100 μM) increases levels of p53, p21Cip1 and Bax proteins in A2780 cells[2].
DNA content of A2780 cells was significantly decreased after 36 and 48 h of pretreatment. The breakdown of DNA results in death of the tumor cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: The human ovarian cancer cell line A2780
Concentration: 5, 10, 30, 50, and 100 μM
Incubation Time: 48 hours
Result: Inhibited cell growth in a time and concentration-dependent manner, IC50=31.1 μM.
Proliferation was significantly inhibited at concentrations of NS1619 higher than 10 μM.

Western Blot Analysis[2]

Cell Line: A2780 cells
Concentration: 0, 5, 10, 30, 50, and 100 μM
Incubation Time: 48 hours
Result: Expression of p53, p21, and Bax in A2780 cells was significantly increased in comparison with control.

Western Blot Analysis[2]

Cell Line: A2780 cells
Concentration: 30 μM
Incubation Time: 36 and 48 hours
Result: DNA content of A2780 cells was significantly decreased after 36 and 48 h of pretreatment. The breakdown of DNA results in death of the tumor cells.
In Vivo

Opening of KCa channels with NS-1619 (1 mg/kg; i.p.) can delay protection in mouse hearts[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male outbred ICR mice[3]
Dosage: 1 mg/kg
Administration: Pretreated i.p. 24 h before I/R
Result: Pretreatment induced delayed protection 24 h later.
Resulted in significant cardioprotection 24 h later, i.e., infarct size was reduced from 38.8 ± 3.7% to 19.8 ± 2.9%.
Molecular Weight

362.23

Formula

C₁₅H₈F₆N₂O₂

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 31 mg/mL (85.58 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7607 mL 13.8034 mL 27.6068 mL
5 mM 0.5521 mL 2.7607 mL 5.5214 mL
10 mM 0.2761 mL 1.3803 mL 2.7607 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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NS-1619
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