O-Acetylserine
Based on 1 Customer Validation
O-Acetylserine (O-Acetyl-L-serine) is an intermediate in the biosynthesis of the amino acid cysteine in bacteria and plants.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 5147-00-2
- Formula: C5H9NO4
- Molecular Weight:147.13
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Human Endogenous Metabolite |
O-acetylserine (OAS) is a central metabolite of sulfur assimilation, providing the carbon backbone for synthesis of cysteine. Sulfide generated through sulfate reduction is incorporated into OAS to form cysteine. OAS displays a signalling function leading to changes in transcript levels of a specific gene set irrespective of the sulfur status of the plant[1].
Treatment with external OAS increases sulfate transporter and adenosine 5′-phosphosulfate reductase gene expression consistent with a model of transcriptional induction by OAS[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5147-00-2
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Appearance Solid
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Molecular Weight 147.13
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Formula C5H9NO4
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Color White to off-white
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SMILES
N[C@@H](COC(C)=O)C(O)=O
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Synonyms
O-Acetyl-L-serine
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
H2O : 50 mg/mL (339.84 mM; Need ultrasonic)
DMSO : 6.25 mg/mL (42.48 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.62 mg/mL (4.21 mM); Clear solution
This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.62 mg/mL (4.21 mM); Clear solution
This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (679.67 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Hubberten HM, et al. Additional role of O-acetylserine as a sulfur status-independent regulator during plant growth. Plant J. 2012 May;70(4):666-77. [Content Brief]
[2]. Hopkins L, et al. O-acetylserine and the regulation of expression of genes encoding components for sulfate uptake and assimilation in potato. Plant Physiol. 2005 May;138(1):433-40. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 6.7967 mL | 33.9836 mL | 67.9671 mL | 169.9178 mL |
| 5 mM | 1.3593 mL | 6.7967 mL | 13.5934 mL | 33.9836 mL | |
| 10 mM | 0.6797 mL | 3.3984 mL | 6.7967 mL | 16.9918 mL | |
| 15 mM | 0.4531 mL | 2.2656 mL | 4.5311 mL | 11.3279 mL | |
| 20 mM | 0.3398 mL | 1.6992 mL | 3.3984 mL | 8.4959 mL | |
| 25 mM | 0.2719 mL | 1.3593 mL | 2.7187 mL | 6.7967 mL | |
| 30 mM | 0.2266 mL | 1.1328 mL | 2.2656 mL | 5.6639 mL | |
| 40 mM | 0.1699 mL | 0.8496 mL | 1.6992 mL | 4.2479 mL | |
| H2O | 50 mM | 0.1359 mL | 0.6797 mL | 1.3593 mL | 3.3984 mL |
| 60 mM | 0.1133 mL | 0.5664 mL | 1.1328 mL | 2.8320 mL | |
| 80 mM | 0.0850 mL | 0.4248 mL | 0.8496 mL | 2.1240 mL | |
| 100 mM | 0.0680 mL | 0.3398 mL | 0.6797 mL | 1.6992 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
O-Acetylserine Related Classifications
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