Secalciferol
Based on 6 publication(s) in Google Scholar
Secalciferol ((24R)-24,25-Dihydroxyvitamin D3) is the major active metabolite of Vitamin D. Secalciferol activates vitamin D receptor (VDR) with an EC50 value of 150 nM. Secalciferol is involved in a wide range of biological functions such as calcium homeostasis, cellular differentiation and proliferation processes, as well as other functions related to the immune system, which is promising for research of rickets, osteomalacia, hypercalcemia and autoimmune disorders.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 55721-11-4
- Formula: C27H44O3
- Molecular Weight:416.64
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Storage:
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Secalciferol
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
Secalciferol of a part of the glucuronides is deconjugated by the enzyme from bacteria in the intestine and is probably reabsorbed and further oxidized to other metabolites in rat intestine[2].
Secalciferol is metabolized by β-glucuronidase to 24,25(OH)2D3-3G and -24G in rat bile[2].
Secalciferol (100 pM-10 μM, 24 h) dose-dependently activates vitamin D receptor (VDR) with an EC50 value of 150 nM, similar to that by Calcitriol (HY-10002) in the IZ-CYP24 and 12-VDRE cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Secalciferol (1 mg/kg, s.c., daily for 28 days) improves the rachitic bone and increases mineralized bone volume in the Hyp mice[1].
Secalciferol (0 or 5 ppm, powder fiet for 57 weeks) induces hyperplasia and neoplasia of the adrenal medulla with increase in cell prpliferative activity in rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Hyp mice[1]
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Dosage:1-1000 μg/kg
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Administration:i.p., daily for 28 days
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Result:Dose dependently increased the length of the fifth lumbar spine in the Hyp mice.
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Animal Model:Hyp mice[1]
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Dosage:1 mg/kg
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Administration:s.c., daily for 28 days and injected Calcein (8 mg/kg) (HY-D0040) on days 15 and 22
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Result:Better improved the rachitic bone in the Hyp mice compared to vihecle.
Chemical Information
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CAS No. 55721-11-4
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Appearance Solid
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Molecular Weight 416.64
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Formula C27H44O3
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Color White to off-white
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SMILES
C=C(CC1)/C(C[C@H]1O)=C\C=C2[C@@]3([H])[C@@](CCC\2)(C)[C@@H]([C@H](C)CC[C@@H](O)C(C)(C)O)CC3
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Synonyms
(24R)-24,25-Dihydroxyvitamin D3
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (6)
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Journal Impact Factor
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Most Recent
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J Clin Invest
Optimal bone fracture repair requires 24R,25-dihydroxyvitamin D3 and its effector molecule FAM57B2. [Abstract]2018 Aug 1;128(8):3546-3557. PMID: 30010626 -
J Lipid Res
Acylation derivatization based LC-MS analysis of 25-hydroxyvitamin D from finger-prick blood. [Abstract]2019 May;60(5):1058-1064. PMID: 30902903 -
Virol Sin
Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo. [Abstract]2024 Aug 19:S1995-820X(24)00134-2. PMID: 39168248 -
Clin Chem Lab Med
Isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedures for the quantification of 24 (R),25-dihydroxyvitamin D2 and 24 (R),25-dihydroxyvitamin D3 in human serum and plasma. [Abstract]2025 Mar 6. PMID: 40042085 -
J Sep Sci
Rapid Determination of 25-Hydroxyvitamin D3 and 24,25-Dihydroxyvitamin D3 in Human Urine Based on Polystyrene/Graphene Oxide Packed-Fibers Solid-Phase Extraction Coupled With High-Performance Liquid Chromatography and Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry. [Abstract]2025 Apr;48(4):e70130. PMID: 40219819 -
Connect Tissue Res
The ER protein TLC domain 3B2 and its enzymatic product lactosylceramide enhance chondrocyte maturation. [Abstract]2019 Aug 28;1-7. PMID: 31462087
Solvent & Solubility
DMSO : 100 mg/mL (240.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 50 mg/mL (120.01 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (585 KB)
- English - EN (585 KB)
- Français - FR (585 KB)
- Deutsch - DE (585 KB)
- Norwegian - NO (585 KB)
- Español - ES (585 KB)
- Swedish - SV (585 KB)
- Italian - IT (585 KB)
- Korean - KR (585 KB)
- Portuguese - PT (585 KB)
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Handling Instructions (2659 KB)
References
[1]. Ono T, et al. 24R,25-dihydroxyvitamin D3 promotes bone formation without causing excessive resorption in hypophosphatemic mice[J]. Endocrinology. 1996 Jun;137(6):2633-7. [Content Brief]
[2]. Higashi T, et al. In vitro and in vivo glucuronidation of 24,25-dihydroxyvitamin D3[J]. Steroids. 1999 Oct;64(10):715-25. [Content Brief]
[3]. Bartonkova I, et al. Profiling of Vitamin D Metabolic Intermediates toward VDR Using Novel Stable Gene Reporter Cell Lines IZ-VDRE and IZ-CYP24[J]. Chem Res Toxicol. 2016 Jul 18;29(7):1211-22. [Content Brief]
[4]. Ikezaki S, et al. Influences of long-term administration of 24R, 25-dihydroxyvitamin D3, a vitamin D3 derivative, in rats[J]. J Toxicol Sci. 1999 May;24(2):133-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| Ethanol / DMSO | 1 mM | 2.4002 mL | 12.0008 mL | 24.0015 mL | 60.0038 mL |
| 5 mM | 0.4800 mL | 2.4002 mL | 4.8003 mL | 12.0008 mL | |
| 10 mM | 0.2400 mL | 1.2001 mL | 2.4002 mL | 6.0004 mL | |
| 15 mM | 0.1600 mL | 0.8001 mL | 1.6001 mL | 4.0003 mL | |
| 20 mM | 0.1200 mL | 0.6000 mL | 1.2001 mL | 3.0002 mL | |
| 25 mM | 0.0960 mL | 0.4800 mL | 0.9601 mL | 2.4002 mL | |
| 30 mM | 0.0800 mL | 0.4000 mL | 0.8001 mL | 2.0001 mL | |
| 40 mM | 0.0600 mL | 0.3000 mL | 0.6000 mL | 1.5001 mL | |
| 50 mM | 0.0480 mL | 0.2400 mL | 0.4800 mL | 1.2001 mL | |
| 60 mM | 0.0400 mL | 0.2000 mL | 0.4000 mL | 1.0001 mL | |
| 80 mM | 0.0300 mL | 0.1500 mL | 0.3000 mL | 0.7500 mL | |
| 100 mM | 0.0240 mL | 0.1200 mL | 0.2400 mL | 0.6000 mL |
Secalciferol Related Classifications
HY-32343 Related Classifications
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