Sirtuin Substrate

Ac-QPKK(Ac)-AMC acetate is a p53-derived peptide conjugated with a fluorophore, which serves as a fluorescent peptide substrate for detecting the deacylase activity of zinc-dependent HDACs and sirtuins (Ex = 360 nm; Em = 460 nm).

2'/3'-O-Acetyl-ADP-ribose sodium is an inhibitor of cell division and oocyte maturation. 2'/3'-O-Acetyl-ADP-ribose sodium causes delay or arrest of oocyte maturation and blastomere division in embryos. 2'/3'-O-Acetyl-ADP-ribose sodium is produced by Sir2 family deacetylases during the coupling of histone/protein deacetylation with β-NAD+ cleavage. In neutral or weakly alkaline aqueous solutions, the direct enzymatic product forms of 2'/3'-O-Acetyl-ADP-ribose sodium, namely the 2'-O-acetyl form and the 3'-O-acetyl form, undergo intramolecular lactone exchange reaction and interconvert with each other.

Ac-QPKK(Ac)-AMC is a p53-derived peptide conjugated with a fluorophore, which serves as a fluorescent peptide substrate for detecting the deacylase activity of zinc-dependent HDACs and sirtuins (Ex = 360 nm; Em = 460 nm).

Z-MAL is a highly efficient and broad-spectrum HDAC substrate. Z-MAL exhibits high conversion activity for class I, II histone deacetylases, and class III SIRT1. Z-MAL can be used in studies on the structure-activity relationship, subtype selectivity, and inhibitor screening of HDAC.