Ac-H-FluNox 

Ac-H-FluNox is an acetylated cell-compatible prodrug of H-FluNox (HY-D2339). Ac-H-FluNox undergoes intracellular hydrolysis of its acetyl group by esterases to generate H-FluNox, which then undergoes a deoxygenation reaction with labile heme to form a fluorescent product. Ac-H-FluNox detects fluctuations of labile heme in living cells, acute labile heme release upon nitric oxide stimulation, and accumulation of labile heme following inhibition of heme export proteins^[1].

Ac-H-FluNox (10 μM; 30 min) detects dose-dependent, NO-induced labile heme release in HeLa cells^[1].
Ac-H-FluNox (10 μM; 30 min) detects labile heme accumulation in FLVCR1a-knockdown SH-SY5Y cells treated with ALA+FAC^[1].
Ac-H-FluNox (10 μM; 30 min) detects elevated labile heme levels in erastin-induced ferroptotic HT1080 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

493.46

C₂₇H₂₁F₂NO₆

2897614-60-5

Solid

White to off-white

O=C1OC2(C3=C1C=CC=C3)C4=CC=C(C=C4OC5=CC([N+]6(CCC(F)(CC6)F)[O-])=CC=C52)OC(C)=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Kawai K, et al. Molecular Imaging of Labile Heme in Living Cells Using a Small Molecule Fluorescent Probe. J Am Chem Soc. 2022;144(9):3793-3803.  [Content Brief]