Ac-RR-AFC TFA
Based on 1 Customer Validation
Ac-RR-AFC TFA is a synthetic fluorogenic Cathepsin B substrate that is cleaved by Cathepsin B and used to determine enzyme activity in cell-free protease assays and cell lysate-based activity assays. Ac-RR-AFC TFA can be used for Cathepsin B activity detection studies.
For research use only. We do not sell to patients.
- Purity : 99.56%
- Formula: C26H33F6N9O7
- Molecular Weight:697.59
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Cathepsin Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
Cathepsin B |
In Vitro
Ac-RR-AFC TFA (29 min) serves as a cathepsin B substrate in recombinant cathepsin B assays, and cathepsin B activity is not inhibited by Toremifene (HY-B0005A), Clarithromycin (HY-17508), Posaconazole (HY-17373), or Mefloquine (HY-17437)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 697.59
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Formula C26H33F6N9O7
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Color White to off-white
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Sequence
Ac-Arg-Arg-AFC
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Sequence Shortening
Ac-RR-AFC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (143.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.4335 mL | 7.1675 mL | 14.3351 mL | 35.8377 mL |
| 5 mM | 0.2867 mL | 1.4335 mL | 2.8670 mL | 7.1675 mL | |
| 10 mM | 0.1434 mL | 0.7168 mL | 1.4335 mL | 3.5838 mL | |
| 15 mM | 0.0956 mL | 0.4778 mL | 0.9557 mL | 2.3892 mL | |
| 20 mM | 0.0717 mL | 0.3584 mL | 0.7168 mL | 1.7919 mL | |
| 25 mM | 0.0573 mL | 0.2867 mL | 0.5734 mL | 1.4335 mL | |
| 30 mM | 0.0478 mL | 0.2389 mL | 0.4778 mL | 1.1946 mL | |
| 40 mM | 0.0358 mL | 0.1792 mL | 0.3584 mL | 0.8959 mL | |
| 50 mM | 0.0287 mL | 0.1434 mL | 0.2867 mL | 0.7168 mL | |
| 60 mM | 0.0239 mL | 0.1195 mL | 0.2389 mL | 0.5973 mL | |
| 80 mM | 0.0179 mL | 0.0896 mL | 0.1792 mL | 0.4480 mL | |
| 100 mM | 0.0143 mL | 0.0717 mL | 0.1434 mL | 0.3584 mL |
Ac-RR-AFC TFA Related Classifications
HY-P11325 Related Classifications
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