2. Metabolic Enzyme/Protease Others
  3. Cathepsin Fluorescent Dye
  4. Ac-RR-AFC TFA

Ac-RR-AFC TFA is a Cathepsin B fluorogenic substrate (Ex=400 nm, Em=505 nm). Cathepsin B activity in cells lysates is determined by measuring cleavage of Ac-RR-AFC TFA and its cleavage occurs at the RR-AFC amide bond. Ac-RR-AFC TFA can be used for activity assays and mechanistic studies on cathepsin B.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-RR-AFC TFA

Ac-RR-AFC TFA Chemical Structure

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Based on 1 publication(s) in Google Scholar

Ac-RR-AFC TFA Related Antibodies

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Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

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Description

Ac-RR-AFC TFA is a Cathepsin B fluorogenic substrate (Ex=400 nm, Em=505 nm). Cathepsin B activity in cells lysates is determined by measuring cleavage of Ac-RR-AFC TFA and its cleavage occurs at the RR-AFC amide bond. Ac-RR-AFC TFA can be used for activity assays and mechanistic studies on cathepsin B[1].

IC50 & Target[1]

Cathepsin B

 

Molecular Weight

697.59

Formula

C26H33F6N9O7
Appearance

Solid

Color

White to off-white

Sequence

Ac-Arg-Arg-AFC
Sequence Shortening

Ac-RR-AFC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (143.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4335 mL 7.1675 mL 14.3351 mL
5 mM 0.2867 mL 1.4335 mL 2.8670 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4335 mL 7.1675 mL 14.3351 mL 35.8377 mL
5 mM 0.2867 mL 1.4335 mL 2.8670 mL 7.1675 mL
10 mM 0.1434 mL 0.7168 mL 1.4335 mL 3.5838 mL
15 mM 0.0956 mL 0.4778 mL 0.9557 mL 2.3892 mL
20 mM 0.0717 mL 0.3584 mL 0.7168 mL 1.7919 mL
25 mM 0.0573 mL 0.2867 mL 0.5734 mL 1.4335 mL
30 mM 0.0478 mL 0.2389 mL 0.4778 mL 1.1946 mL
40 mM 0.0358 mL 0.1792 mL 0.3584 mL 0.8959 mL
50 mM 0.0287 mL 0.1434 mL 0.2867 mL 0.7168 mL
60 mM 0.0239 mL 0.1195 mL 0.2389 mL 0.5973 mL
80 mM 0.0179 mL 0.0896 mL 0.1792 mL 0.4480 mL
100 mM 0.0143 mL 0.0717 mL 0.1434 mL 0.3584 mL
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Ac-RR-AFC TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-RR-AFCCathepsinFluorescent DyeCathepsin BFluorogenic substrateRR-AFC amide bondInhibitorinhibitorinhibit

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