AGMO-IN-1

AGMO-IN-1 (300 µM; 0-3 d post-IDI for cell cycle analysis; 0-7 d post-IDI for cyclin mRNA detection) slows cell cycle progression of 3T3-L1 cells during IDI-induced clonal expansion, increasing the S phase at 1 d and the G2 phase at 3 d^[1].

AGMO-IN-1 (0.1-1000 µM; 24 h) does not further reduce the viability of shAGMO RAW264.7 macrophages and only modestly reduces shLUC/+huAGMO readings at high concentrations^[1].

AGMO-IN-1 (100 µM; 48 h) blocks IL-4-driven M2 polarization in +huAGMO RAW264.7 macrophages and enhances some M1-like markers^[1].

AGMO-IN-1 (3-10,000 µM; preincubation for 10 min; reaction for 30 min) inhibits NOS activity in RAW264.7 macrophage lysates with an IC₅₀ of 1.9-5.3 mM, indicating lower potency than against AGMO^[1].

AGMO-IN-1 (300-1000 µM; pretreatment for 48 h starting from 72 h; continuous exposure until day 6; washout on days 7-10; endpoint detection on day 10) reversibly inhibits 3T3-L1 proliferation, but at 1 mM irreversibly prevents adipocyte lipid droplet formation, whereas 300 µM reduces differentiation markers^[1].
AGMO-IN-1 (100-300 µM; 24 h) reduces FFA-induced lipid droplet accumulation in HepG2 hepatocytes but does not prevent FFA-induced loss of viability^[1].
AGMO-IN-1 (300 µM; differentiation days 0-7) is characterized during 3T3-L1 adipocyte differentiation, and Pref-1/FABP4 marker expression and morphology are monitored until day 7^[1].
AGMO-IN-1 (300 µM; day 7 of differentiation) produces BODIPY-488-positive lipid droplets in 3T3-L1 adipocytes^[1].
AGMO-IN-1 (300 µM; days 5-7 of differentiation, combined time points) is associated with changes in Oil Red O lipid titer quantification in differentiating 3T3-L1 adipocytes^[1].

AGMO-IN-1 (compound Cp6) (preincubation 10 min; reaction 20 min) inhibits recombinant rat PAH only with an IC₅₀ of 360-430 µM, showing that its potency against PAH is lower than that against AGMO^[1].

AGMO-IN-1 (10 min preincubation; 60 min reaction) inhibits rat liver microsomal AGMO with an IC₅₀ of 26-100 µM, and the half-maximal effect is approximately 50 µM^[1].
AGMO-IN-1 (0.1-1000 µM; 24 h) reduces the viability/proliferation of low-density 3T3-L1 cells with an IC₅₀ of 300-400 µM, but has no effect at high culture density^[1].