Alisol C
Based on 1 Customer Validation
Alisol C is a triterpenoid that can be found in the tuber of Alisma orientale (Sam.) Juzep.
For research use only. We do not sell to patients.
- Purity: 99.61%
- CAS No.: 30489-27-1
- Formula: C30H46O5
- Molecular Weight:486.68
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Triterpenes are a class of terpenoid compounds characterized by a fundamental core structure composed of 30 carbon atoms; they exist within plants either in a free form or bound to sugars as glycosides or esters.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 30489-27-1
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Appearance Solid
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Molecular Weight 486.68
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Formula C30H46O5
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Color White to off-white
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SMILES
C[C@@]12[C@@]3(C(C[C@@H]([C@@]1([H])[C@@]4([C@@](C(C)(C(CC4)=O)C)([H])CC2)C)O)=C(C(C3)=O)[C@H](C)C[C@@H]([C@]5([H])C(C)(O5)C)O)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (205.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.14 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Tai Y, et al. Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESI/APCI-MS/MS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature. J Anal Methods Chem. 2019;2019:8320171. Published 2019 Aug 14. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0547 mL | 10.2737 mL | 20.5474 mL | 51.3685 mL |
| 5 mM | 0.4109 mL | 2.0547 mL | 4.1095 mL | 10.2737 mL | |
| 10 mM | 0.2055 mL | 1.0274 mL | 2.0547 mL | 5.1368 mL | |
| 15 mM | 0.1370 mL | 0.6849 mL | 1.3698 mL | 3.4246 mL | |
| 20 mM | 0.1027 mL | 0.5137 mL | 1.0274 mL | 2.5684 mL | |
| 25 mM | 0.0822 mL | 0.4109 mL | 0.8219 mL | 2.0547 mL | |
| 30 mM | 0.0685 mL | 0.3425 mL | 0.6849 mL | 1.7123 mL | |
| 40 mM | 0.0514 mL | 0.2568 mL | 0.5137 mL | 1.2842 mL | |
| 50 mM | 0.0411 mL | 0.2055 mL | 0.4109 mL | 1.0274 mL | |
| 60 mM | 0.0342 mL | 0.1712 mL | 0.3425 mL | 0.8561 mL | |
| 80 mM | 0.0257 mL | 0.1284 mL | 0.2568 mL | 0.6421 mL | |
| 100 mM | 0.0205 mL | 0.1027 mL | 0.2055 mL | 0.5137 mL |