ALK-IN-40
ALK-IN-40 is a potent and selective ALK inhibitor with IC50 values of 8.52 nM and 6.84 nM for ALK and ALK-G1202R, respectively. ALK-IN-40 inhibits cancer cell proliferation by blocking phosphorylation of wild-type and mutant ALK and downstream AKT. ALK-IN-40 can be used for research on ALK-positive metastatic non-small cell lung cancer (NSCLC).
For research use only. We do not sell to patients.
- Formula: C25H29ClN4O6S
- Molecular Weight:549.04
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
ALK 8.52 nM (IC50) |
ALK-G1202R 6.84 nM (IC50) |
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| BaF3 | GI50 |
1.45 μM
|
Antiproliferative activity against Ba/F3-EML4-ALK-WT cells assessed as viability reduction incubated 72 hrs by CCK-8 assay.
Antiproliferative activity against Ba/F3-EML4-ALK-WT cells assessed as viability reduction incubated 72 hrs by CCK-8 assay.
|
42911680 |
| BaF3 | GI50 |
2.66 μM
|
Antiproliferative activity against Ba/F3-EML4-ALK-G1202R cells assessed as viability reduction incubated 72 hrs by CCK-8 assay.
Antiproliferative activity against Ba/F3-EML4-ALK-G1202R cells assessed as viability reduction incubated 72 hrs by CCK-8 assay.
|
42911680 |
In Vitro
ALK-IN-40 (72 h) inhibits the proliferation of Ba/F3-EML4-ALK-WT and Ba/F3-EML4-ALK-G1202R cells with GI50 values of 1.45 and 2.66 µM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Ba/F3-EML4-ALK-G1202R cells
-
Concentration:0.016, 0.08, 0.4, 2, and 10 µM
-
Incubation Time:3 h
-
Result:Reduced phosphorylation of ALK-G1202R and downstream AKT at relatively high concentrations.
- Western Blot
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- Kinase activity and phosphorylation assays
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Protocol for Kinase activity and phosphorylation assays
- MTT Cell Proliferation Assay
Chemical Information
-
Molecular Weight 549.04
-
Formula C25H29ClN4O6S
-
SMILES
O=C(C1=CC(OC(C)C)=C(NC2=NC=C(Cl)C(NC3=CC=CC=C3S(=O)(C(C)C)=O)=N2)C=C1C)C(O)O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ALK-IN-40 Related Classifications
HY-190791 Related Classifications
Powered by Bioz