APGW-amide
APGW-amide is a neuropeptide. APGW-amide controls the growth of imposex structures and promotes growth in Haliotis asinina. APGW-amide inhibits Achatina neurons and modulates neuronal currents induced by neuroactive compounds. APGW-amide induces a rapid increase in [Ca2+]i in Fura-2/AM-loaded PC12 cells. APGW-amide is useful for research on the nervous system.
For research use only. We do not sell to patients.
- CAS No.: 126675-52-3
- Formula: C21H28N6O4
- Molecular Weight:428.48
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
In Vitro
APGW-amide (3 μM) increases the membrane conductance (g) value measured before the Iout caused by erythro-l-BHGA, with ED50, slope, Hill coefficient and Emax were 76.9 msec, 0.89274, 1.59453, and 5.54 nA, respectively[1].
APGW-amide (3 μM) inhibits the Iout, with ED50, slope, Hill coefficient and Emax were 29.5 msec, 1.02386, 1.9761, and 0.38 nA, respectively, increases the g value measured before the Iout induced by Dopamine (HY-B0451)[1].
APGW-amide (3 μM) inhibits the fast component of the Iin caused by Serotonin (HY-B1473A), with ED50, slope, Hill coefficient and Emax were 18.5 msec, 0.8268, 1.6557, and 0.72 nA, respectively[1].
APGW-amide (30 μM) causes a rapid rise of [Ca2+]i in Fura-2/AM-loaded PC12 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
APGW-amide (0.0000001-0.01 nM, every other day, 14 days) induces imposex in the mud snail, Ilyanassa obsoleta[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 126675-52-3
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Molecular Weight 428.48
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Formula C21H28N6O4
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Sequence
Ala-Pro-Gly-Trp-NH2
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Sequence Shortening
APGW-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Purity & Documentation
References
[1]. Han XY, et al. Modulation by APGW-amide, an Achatina endogenous inhibitory tetrapeptide, of currents induced by neuroactive compounds on Achatina neurons: amines and amino acids. Gen Pharmacol. 1997 Oct;29(4):523-30. [Content Brief]
[2]. Han XY, et al. Effect of APGW-amide on [Ca2+]i in rat pheochromocytoma PC12 cells. Jpn J Pharmacol. 2000 Nov;84(3):355-9. [Content Brief]
[4]. Oberdörster E, et al.The neuropeptide APGWamide induces imposex in the mud snail, Ilyanassa obsoleta. Peptides. 2000 Sep;21(9):1323-30. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
APGW-amide Related Classifications
HY-P11082 Related Classifications
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