2. Membrane Transporter/Ion Channel
  3. Aquaporin
  4. AqF026

AqF026 is an aquaporin 1 (AQP1) agonist. AqF026 enhances the channel activity of human AQP1 in the Xenopus laevis oocyte system. AqF026 enhances the osmotic transport of water across the peritoneum in a mouse model of peritoneal dialysis. AqF026 can be used in studies related to ultrafiltration failure in peritoneal dialysis.

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AqF026

AqF026 Chemical Structure

CAS No. : 1021869-59-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

AqF026 Related Antibodies

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Description

AqF026 is an aquaporin 1 (AQP1) agonist. AqF026 enhances the channel activity of human AQP1 in the Xenopus laevis oocyte system. AqF026 enhances the osmotic transport of water across the peritoneum in a mouse model of peritoneal dialysis. AqF026 can be used in studies related to ultrafiltration failure in peritoneal dialysis[1][2].

In Vitro

AqF026 (0.1-100 μM) directly enhances human AQP1-mediated water transport in Xenopus laevis oocytes, with an EC50 of 3.3 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AqF026 Related Antibodies
In Vivo

AqF026 (0.75-30 μM; i.v.; single injection 30 minutes prior to dialysis assay) directly and specifically potentiates AQP1-mediated osmotic water transport in a mouse peritoneal dialysis model, producing a maximal 15% increase in net ultrafiltration at 15 μM with an EC50 of 4.2 μM, with no effect on solute transport or AQP1 expression[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wild-type (Aqp1+/+) (sex-matched littermates, 8-12 weeks old); Aqp1-null (Aqp1-/-) (sex-matched littermates, 8-12 weeks old)[1]
Dosage: 0.75 μM; 1.5 μM; 7.5 μM; 15 μM; 30 μM
Administration: i.v.; single injection 30 minutes prior to dialysis assay
Result: Produced a dose-dependent increase in net ultrafiltration in wild-type mice, with a maximal 15% increase at 15 μM and an estimated EC50 of 4.2 μM.
Increased the initial ultrafiltration rate in wild-type mice by ~50% (32.2 μL/min at 15 μM.
Significantly increased intraperitoneal volume at 30, 60, and 90 minutes at 15 μM.
Enhanced sodium sieving in wild-type mice, with a greater dip in dialysate sodium concentration at 30 minutes (11.2 mEq/L) at 15 μM.
Had no effect on net ultrafiltration or initial ultrafiltration rates in Aqp1-null mice.
Did not alter the osmotic gradient, small solute transport (urea and glucose), AQP1 protein/mRNA expression, or AQP1 plasma membrane localization in wild-type mice.
Molecular Weight

434.89

Formula

C20H19ClN2O5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC(S(=O)(NCC2=CC=CC=C2)=O)=C(C=C1NCC3=CC=CO3)Cl)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (229.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2994 mL 11.4972 mL 22.9943 mL
5 mM 0.4599 mL 2.2994 mL 4.5989 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2994 mL 11.4972 mL 22.9943 mL 57.4858 mL
5 mM 0.4599 mL 2.2994 mL 4.5989 mL 11.4972 mL
10 mM 0.2299 mL 1.1497 mL 2.2994 mL 5.7486 mL
15 mM 0.1533 mL 0.7665 mL 1.5330 mL 3.8324 mL
20 mM 0.1150 mL 0.5749 mL 1.1497 mL 2.8743 mL
25 mM 0.0920 mL 0.4599 mL 0.9198 mL 2.2994 mL
30 mM 0.0766 mL 0.3832 mL 0.7665 mL 1.9162 mL
40 mM 0.0575 mL 0.2874 mL 0.5749 mL 1.4371 mL
50 mM 0.0460 mL 0.2299 mL 0.4599 mL 1.1497 mL
60 mM 0.0383 mL 0.1916 mL 0.3832 mL 0.9581 mL
80 mM 0.0287 mL 0.1437 mL 0.2874 mL 0.7186 mL
100 mM 0.0230 mL 0.1150 mL 0.2299 mL 0.5749 mL
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AqF026 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AqF0261021869-59-9AqF 026AqF-026AquaporinAQPAQP1 agonistXenopus laevis oocyte systemmicePeritoneal dialysis
Inhibitorinhibitorinhibit

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