Cat. No.: HY-134909

AS-136A

Customer Review

Based on 1 Customer Validation

AS-136A is an orally active non-nucleoside inhibitor of the measles virus RNA-dependent RNA polymerase (RdRp) with an IC₅₀ of 2 µM for measles virus.

For research use only. We do not sell to patients.

Purity: 98.08%
CAS No.: 949898-66-2
Formula: C₁₇H₁₉F₃N₄O₃S
Molecular Weight:416.42

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

AS-136A Chemical Structure

CAS No.: 949898-66-2

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

AS-136A is an orally active non-nucleoside inhibitor of the measles virus RNA-dependent RNA polymerase (RdRp) with an IC₅₀ of 2 µM for measles virus^[1].

IC₅₀ & Target

RNA Polymerase

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Vero	CC₅₀	>300 μM Compound: 14d	Cytotoxicity against Vero cells by MTT assay Cytotoxicity against Vero cells by MTT assay	[PMID: 17643302]
Vero	CC₅₀	>300 μM Compound: 15a	Cytotoxicity against african green monkey Vero cells by MTT assay Cytotoxicity against african green monkey Vero cells by MTT assay	[PMID: 18529043]
Vero	CC₅₀	>75 μM Compound: 2, AS-136a	Cytotoxicity against african green monkey Vero cells after 24 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 24 hrs by MTT assay	[PMID: 22480182]
Vero	CC₅₀	199 μM Compound: 14d	Cytotoxicity against Vero cells by Trypan blue exclusion assay Cytotoxicity against Vero cells by Trypan blue exclusion assay	[PMID: 17643302]
Vero	CC₅₀	199 μM Compound: 15a	Cytotoxicity against african green monkey Vero cells by trypan blue exclusion assay Cytotoxicity against african green monkey Vero cells by trypan blue exclusion assay	[PMID: 18529043]
Vero	EC₅₀	<2.3 μM Compound: 15a	Antiviral activity against Measles virus Alaska in Vero cells assessed as inhibition of cytopathic effect Antiviral activity against Measles virus Alaska in Vero cells assessed as inhibition of cytopathic effect	[PMID: 18529043]
Vero	EC₅₀	0.014 μM Compound: 15a	Antiviral activity against Measles virus Alaska in Vero cells assessed as reduction of viral titer Antiviral activity against Measles virus Alaska in Vero cells assessed as reduction of viral titer	[PMID: 18529043]
Vero	EC₅₀	0.014 μM Compound: 2, AS-136a	Antiviral activity against Measles virus Alaska infected african green monkey Vero cells expressing human SLAM assessed as decrease in viral titer at EC50 of cytopathic inhibition Antiviral activity against Measles virus Alaska infected african green monkey Vero cells expressing human SLAM assessed as decrease in viral titer at EC50 of cytopathic inhibition	[PMID: 22480182]
Vero	EC₅₀	2 μM Compound: 2, AS-136a	Antiviral activity against Measles virus Alaska infected african green monkey Vero cells expressing human SLAM assessed as decrease in virus-induced cytopathic effect Antiviral activity against Measles virus Alaska infected african green monkey Vero cells expressing human SLAM assessed as decrease in virus-induced cytopathic effect	[PMID: 22480182]

Chemical Information

CAS No. 949898-66-2
Appearance Solid
Molecular Weight 416.42
Formula C₁₇H₁₉F₃N₄O₃S
Color White to off-white
SMILES

O=C(C1=CC(C(F)(F)F)=NN1C)NC2=CC=C(S(=O)(N3CCCCC3)=O)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (240.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4014 mL

12.0071 mL

24.0142 mL

5 mM

0.4803 mL

2.4014 mL

4.8028 mL

10 mM

0.2401 mL

1.2007 mL

2.4014 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (12.01 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ndungu JM, et al. Non-nucleoside inhibitors of the measles virus RNA-dependent RNA polymerase: synthesis, structure-activity relationships, and pharmacokinetics. J Med Chem. 2012;55(9):4220-4230. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4014 mL	12.0071 mL	24.0142 mL	60.0355 mL
	5 mM	0.4803 mL	2.4014 mL	4.8028 mL	12.0071 mL
	10 mM	0.2401 mL	1.2007 mL	2.4014 mL	6.0036 mL
	15 mM	0.1601 mL	0.8005 mL	1.6009 mL	4.0024 mL
	20 mM	0.1201 mL	0.6004 mL	1.2007 mL	3.0018 mL
	25 mM	0.0961 mL	0.4803 mL	0.9606 mL	2.4014 mL
	30 mM	0.0800 mL	0.4002 mL	0.8005 mL	2.0012 mL
	40 mM	0.0600 mL	0.3002 mL	0.6004 mL	1.5009 mL
	50 mM	0.0480 mL	0.2401 mL	0.4803 mL	1.2007 mL
	60 mM	0.0400 mL	0.2001 mL	0.4002 mL	1.0006 mL
	80 mM	0.0300 mL	0.1501 mL	0.3002 mL	0.7504 mL
	100 mM	0.0240 mL	0.1201 mL	0.2401 mL	0.6004 mL

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AS-136A

MCE Bioactive Compound Library

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

AS-136A Related Classifications

HY-134909 Related Classifications