Art. -Nr.: HY-W012311

3′-Deoxythymidine

(Synonyms: 2′,3′-Dideoxythymidine; ddThd)

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3′-Deoxythymidine (2′,3′-Dideoxythymidine), a nucleoside analog, inhibits eukaryotic cellular and viral DNA polymerases, and inhibits retrovirus infection in some cells.

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Reinheit: 99.13%
CAS. Nr.: 3416-05-5
Formel: C₁₀H₁₄N₂O₄
Molecular Weight:226.23

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

3′-Deoxythymidine Chemical Structure

CAS. Nr.: 3416-05-5

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

3′-Deoxythymidine (2′,3′-Dideoxythymidine), a nucleoside analog, inhibits eukaryotic cellular and viral DNA polymerases, and inhibits retrovirus infection in some cells^[1].

IC₅₀ & Target

DNA Polymerase

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
ATH-8 cell line	ED₅₀	100 μM Compound: 6a	Protection of ATH8 cells against the cytopathic effect of HIV. Protection of ATH8 cells against the cytopathic effect of HIV.	[PMID: 3497272]
CCRF-CEM	IC₅₀	0.38 μM Compound: 5	Concentration of compound required to inhibit 50% of viral replication of human immunodeficiency virus in CEM-F cells Concentration of compound required to inhibit 50% of viral replication of human immunodeficiency virus in CEM-F cells	[PMID: 2754712]
CEM-SS	CC₅₀	>100 μM Compound: ddT	Cytotoxicity against human CEM-SS cells assessed as reduction in cell viability Cytotoxicity against human CEM-SS cells assessed as reduction in cell viability	[PMID: 34695774]
CEM-TK(-)	CC₅₀	>100 μM Compound: ddT	Cytotoxicity against human CEM-TK(-) cells assessed as reduction in cell viability Cytotoxicity against human CEM-TK(-) cells assessed as reduction in cell viability	[PMID: 34695774]
MT2	EC₅₀	>200 μM Compound: 4	Inhibition of virus-induced cytopathic effect in wild type HIV 3a infected MT2 cells after 5 days Inhibition of virus-induced cytopathic effect in wild type HIV 3a infected MT2 cells after 5 days	[PMID: 17562366]
MT4	CC₅₀	>100 μM Compound: ddT	Cytotoxicity against human MT4 cells assessed as reduction in cell viability Cytotoxicity against human MT4 cells assessed as reduction in cell viability	[PMID: 34695774]
MT4	ED₅₀	6 μM Compound: 4	Concentration required to affect a 50% reduction in the cytopathic effect of HIV for MT-4 cells Concentration required to affect a 50% reduction in the cytopathic effect of HIV for MT-4 cells	[PMID: 1527788]

Chemical Information

CAS. Nr. 3416-05-5
Appearance Solid
Molecular Weight 226.23
Formel C₁₀H₁₄N₂O₄
Color White to off-white
SMILES

O=C(NC(C(C)=C1)=O)N1[C@@H]2O[C@H](CO)CC2
Synonyms

2′,3′-Dideoxythymidine; ddThd
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (442.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.4203 mL

22.1014 mL

44.2028 mL

5 mM

0.8841 mL

4.4203 mL

8.8406 mL

10 mM

0.4420 mL

2.2101 mL

4.4203 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (22.10 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (22.10 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (249 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.4203 mL	22.1014 mL	44.2028 mL	110.5070 mL
	5 mM	0.8841 mL	4.4203 mL	8.8406 mL	22.1014 mL
	10 mM	0.4420 mL	2.2101 mL	4.4203 mL	11.0507 mL
	15 mM	0.2947 mL	1.4734 mL	2.9469 mL	7.3671 mL
	20 mM	0.2210 mL	1.1051 mL	2.2101 mL	5.5254 mL
	25 mM	0.1768 mL	0.8841 mL	1.7681 mL	4.4203 mL
	30 mM	0.1473 mL	0.7367 mL	1.4734 mL	3.6836 mL
	40 mM	0.1105 mL	0.5525 mL	1.1051 mL	2.7627 mL
	50 mM	0.0884 mL	0.4420 mL	0.8841 mL	2.2101 mL
	60 mM	0.0737 mL	0.3684 mL	0.7367 mL	1.8418 mL
	80 mM	0.0553 mL	0.2763 mL	0.5525 mL	1.3813 mL
	100 mM	0.0442 mL	0.2210 mL	0.4420 mL	1.1051 mL

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3′-Deoxythymidine

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

Nucleotide Compound Library

Anti-Lung Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

3D Diverse Fragment Library

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Help & FAQs

Complete Stock Solution Preparation Table

3′-Deoxythymidine

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

Nucleotide Compound Library

Anti-Lung Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

3D Diverse Fragment Library

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Help & FAQs

Complete Stock Solution Preparation Table

3′-Deoxythymidine Related Classifications

HY-W012311 Related Classifications