Art. -Nr.: HY-50709

A939572

41 Cited Publications

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Based on 41 publication(s) in Google Scholar

A939572 is a potent, and orally bioavailable stearoyl-CoA desaturase1 (SCD1) inhibitor with IC₅₀ values of <4 nM and 37 nM for mSCD1 and hSCD1, respectively.

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Reinheit: 99.39%
CAS. Nr.: 1032229-33-6
Formel: C₂₀H₂₂ClN₃O₃
Molecular Weight:387.86

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

A939572 Chemical Structure

CAS. Nr.: 1032229-33-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (41)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

A939572 is a potent, and orally bioavailable stearoyl-CoA desaturase1 (SCD1) inhibitor with IC₅₀ values of <4 nM and 37 nM for mSCD1 and hSCD1, respectively.

IC₅₀ & Target

IC50: <4 nM (mSCD1), 37 nM (hSCD1)^[1]

In Vitro

A939572 exhibits robust in vivo activity with dose-dependent desaturation index lowering effects^[1]. A939572 is a small molecule that specifically inhibits SCD1 enzymatic activity. A939572 demonstrates a significant dose-dependent decrease in proliferation in Caki1, A498, Caki2, and ACHN at day 5 (IC₅₀s of 65 nM, 50 nM, 65 nM, and 6 nM, respectively). In A939572 (SCDi) treated Caki1 and A498 cells, all five ER stress related genes are expressed at significantly increased levels compared to DMSO+BSA control, and this elevated expression can be blocked with the addition of OA-BSA^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Athymic nude (nu/nu) mice bearing A498 ccRCC xenografts are treated with A939572 (30mg/kg, p.o.) and Tem individually or in combination over the course of four weeks, and tumor volume (mm³) is recorded. A939572 and Tem monotherapy generate similar growth responses with approximately 20-30% reductions in tumor volume (vs. placebo control) being observed upon study completion, with values reaching statistical significance only within the last week of treatment. The combination group yields over a 60% decrease in tumor volume (vs. placebo control) by study completion with significant reductions recorded after approximately 1 week of treatment^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1032229-33-6
Appearance Solid
Molecular Weight 387.86
Formel C₂₀H₂₂ClN₃O₃
Color White to off-white
SMILES

O=C(N1CCC(OC2=C(Cl)C=CC=C2)CC1)NC3=CC=CC(C(NC)=O)=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (41)

All Cell Proliferation/Viability AssayWB

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Journal Impact Factor

Gastroenterology

Oncogenic Fatty Acid Metabolism Rewires Energy Supply Chain in Gastric Carcinogenesis. [Abstract]

2024 Jan 24:S0016-5085(24)00064-7. PMID: 38272100
Cancer Res

Retinol saturase mediates retinoid metabolism to impair a ferroptosis defense system in cancer cells. [Abstract]

2023 Jul 14;83(14):2387-2404. PMID: 37184371
Nat Commun

PPARα-mediated lipid metabolism reprogramming supports anti-EGFR therapy resistance in head and neck squamous cell carcinoma. [Abstract]

2025 Feb 1;16(1):1237. PMID: 39890801
Nat Commun

Acid-exposed and hypoxic cancer cells do not overlap but are interdependent for unsaturated fatty acid resources. [Abstract]

2024 Nov 21;15(1):10107. PMID: 39572570
Nat Commun

Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice. [Abstract]

2024 Sep 27;15(1):8273. PMID: 39333556
Acta Pharm Sin B

Intestinal stearoyl-coenzyme A desaturase-inhibition improves obesity-associated metabolic disorders. [Abstract]

2025 Feb;15(2):892-908. PMID: 40177566
Cell Death Dis

A targetable antioxidant defense mechanism to EZH2 inhibitors enhances tumor cell vulnerability to ferroptosis. [Abstract]

2025 Apr 14;16(1):291. PMID: 40229247
Cancer Lett

YTHDF2-mediated stabilization of SREBF1 promotes lipid metabolic reprogramming and ferroptosis-associated radioresistance in anaplastic thyroid carcinoma. [Abstract]

2025 Dec 21:639:218232. PMID: 41435981
Int J Biol Sci

Stearoyl-CoA Desaturase-1 dependent lipid droplets accumulation in cancer-associated fibroblasts facilitates the progression of lung cancer. [Abstract]

2022 Oct 18;18(16):6114-6128. PMID: 36439884
J Exp Med

Liver X receptors are required for thymic resilience and T cell output. [Abstract]

2020 Oct 5;217(10):e20200318. PMID: 32716519
Sci China Life Sci

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry. [Abstract]

2022 Feb;65(2):341-361. PMID: 34047913
Oncogene

Betulinic acid induces a novel cell death pathway that depends on cardiolipin modification. [Abstract]

2016 Jan 28;35(4):427-37. PMID: 25893306

A939572 purchased from MedChemExpress. Usage Cited in: Oncogene. 2016 Jan 28;35(4):427-37. [Abstract]

HeLa cells are treated with vehicle (DMSO) or 200 nM SCD-1 inhibitor for 40 h after which cells are analysed via TEM.
Mol Med

G protein-coupled estrogen receptor activates PI3K/AKT/mTOR signaling to suppress ferroptosis via SREBP1/SCD1-mediated lipogenesis. [Abstract]

2024 Feb 21;30(1):28. PMID: 38383297
Br J Cancer

Stearoyl-CoA-desaturase-1 regulates gastric cancer stem-like properties and promotes tumour metastasis via Hippo/YAP pathway. [Abstract]

2020 Jun;122(12):1837-1847. PMID: 32350414
Cell Rep

Concurrent Mutations in STK11 and KEAP1 Promote Ferroptosis Protection and SCD1 Dependence in Lung Cancer. [Abstract]

2020 Dec 1;33(9):108444. PMID: 33264619
Cancer Cell Int

Stearoyl CoA desaturase inhibition can effectively induce apoptosis in bladder cancer stem cells. [Abstract]

2024 Oct 29;24(1):357. PMID: 39472909
Cancer Cell Int

SCD1 is required for EGFR-targeting cancer therapy of lung cancer via re-activation of EGFR/PI3K/AKT signals. [Abstract]

2019 Apr 18:19:103. PMID: 31019378
J Ethnopharmacol

Paris polyphylla Smith var. yunnanensis-derived saponins potentiate the antitumor activity of GPX4 inhibitors. [Abstract]

2026 Feb 28:357:120890. PMID: 41232632
Biochem Pharmacol

Ghrelin attenuates metabolic dysfunction-associated steatotic liver disease by activating glutamine metabolism via inhibition of the IRE1α-XBP-1 pathway. [Abstract]

2025 Jun 26:240:117089. PMID: 40581331
Mol Metab

Loss of mitochondrial aconitase promotes colorectal cancer progression via SCD1-mediated lipid remodeling. [Abstract]

2021 Jun:48:101203. PMID: 33676027
Cells

Targeting Wnt/β-Catenin Signaling Exacerbates Ferroptosis and Increases the Efficacy of Melanoma Immunotherapy via the Regulation of MITF. [Abstract]

2022 Nov 12;11(22):3580. PMID: 36429010
Cancer Metab

CD36 inhibition enhances the anti-proliferative effects of PI3K inhibitors in PTEN-loss anti-HER2 resistant breast cancer cells. [Abstract]

2025 Feb 7;13(1):6. PMID: 39920872
Eur J Pharmacol

Identification and experimental validation of Stearoyl-CoA desaturase is a new drug therapeutic target for osteosarcoma. [Abstract]

2024 Jan 15:963:176249. PMID: 38070637
Respir Res

TGF-β1 promotes SCD1 expression via the PI3K-Akt-mTOR-SREBP1 signaling pathway in lung fibroblasts. [Abstract]

2023 Jan 10;24(1):8. PMID: 36627645
Int J Mol Sci

Stearoyl-CoA Desaturases1 Accelerates Non-Small Cell Lung Cancer Metastasis by Promoting Aromatase Expression to Improve Estrogen Synthesis. [Abstract]

2023 Apr 6;24(7):6826. PMID: 37047797
J Pathol

ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p. [Abstract]

2020 Dec;252(4):358-370. PMID: 32770671
Biol Res

RASAL1 induces to downregulate the SCD1, leading to suppression of cell proliferation in colon cancer via LXRα/SREBP1c pathway. [Abstract]

2019 Dec 17;52(1):60. PMID: 31847887
Front Pharmacol

SCD1 Confers Temozolomide Resistance to Human Glioma Cells via the Akt/GSK3β/β-Catenin Signaling Axis. [Abstract]

2018 Jan 4:8:960. PMID: 29354058

A939572 purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Jan 4:8:960. [Abstract]

Western blot for p-Akt (Ser-473), Akt, p-GSK3β (Ser-9), GSK3β, β-catenin, and SCD1 in T98G-R cells treated with DMSO, A939572, MK2206, A939572 plus MK2206, LY294002 and A939572 plus LY294002.

A939572 purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Jan 4:8:960. [Abstract]

Western blot for p-Akt (Ser-473), Akt, p-GSK3β (Ser-9), GSK3β, β-catenin, and SCD1 in U87-R cells treated with DMSO, A939572, MK2206, A939572 plus MK2206, LY294002 and A939572 plus LY294002.
Chem Biol Interact

Sulfur mustard exposure induces inflammatory injury by Dnmt3b-mediated DNA methylation via oleic acid accumulation in Treg cells. [Abstract]

2026 May 27:112177. PMID: 42208724
PLoS Pathog

Stearoly-CoA desaturase 1 differentiates early and advanced dengue virus infections and determines virus particle infectivity. [Abstract]

2018 Aug 17;14(8):e1007261. PMID: 30118512
Stem Cells

Runx1 Role in Epithelial and Cancer Cell Proliferation Implicates Lipid Metabolism and Scd1 and Soat1 Activity. [Abstract]

2018 Oct;36(10):1603-1616. PMID: 29938858
Med Oncol

Upregulation of SCD1 by ErbB2 via LDHA promotes breast cancer cell migration and invasion. [Abstract]

2022 Dec 5;40(1):40. PMID: 36471172
J Cell Mol Med

Inhibition of stearoyl CoA desaturase-1 activity suppresses tumour progression and improves prognosis in human bladder cancer. [Abstract]

2019 Mar;23(3):2064-2076. PMID: 30592142

A939572 purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2019 Mar;23(3):2064-2076. [Abstract]

Cyclins D1, Rb, PCNA, Cdk4 and Cdk6 are down-regulated after either 24 h treatment of A939572 or siRNAs, while cyclin E1 is up-regulated.

A939572 purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2019 Mar;23(3):2064-2076. [Abstract]

Compared to the DMSO (0.25%) group, A939572 at concentrations of 6.4 and 12.8 μg/mL up-regulate E-cadherin expression and down-regulate N-cadherin expression.
Funct Integr Genomics

Deltex E3 ubiquitin ligase 4 promotes thyroid cancer progression through stearoyl-CoA desaturase 1. [Abstract]

2023 Aug 23;23(3):280. PMID: 37612343
Biochim Biophys Acta Mol Cell Biol Lipids

Trichothecin inhibits invasion and metastasis of colon carcinoma associating with SCD-1-mediated metabolite alteration. [Abstract]

2020 Feb;1865(2):158540. PMID: 31678511
Front Oncol

A Novel Ferroptosis-Related Gene Model for Overall Survival Predictions of Bladder Urothelial Carcinoma Patients. [Abstract]

2021 Jul 27:11:698856. PMID: 34386423
Hum Cell

Establishment and drug resistance characterization of paired organoids using human primary colorectal cancer and matched tumor deposit specimens. [Abstract]

2024 Nov 4;38(1):13. PMID: 39495391
Biol Pharm Bull

Inhibition of Stearoyl-CoA Desaturase 1 Potentiates Anti-tumor Activity of Amodiaquine in Non-small Cell Lung Cancer. [Abstract]

2022 Apr 1;45(4):438-445. PMID: 35110426
SSRN

23 Nov 2020.
bioRxiv

2020 Mar.
Oncotarget

Targeting stearoyl-CoA desaturase 1 to repress endometrial cancer progression. [Abstract]

2018 Jan 24;9(15):12064-12078. PMID: 29552293

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (257.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5782 mL

12.8912 mL

25.7825 mL

5 mM

0.5156 mL

2.5782 mL

5.1565 mL

10 mM

0.2578 mL

1.2891 mL

2.5782 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[2]

Cells are plated (0.5 or 1×10⁵/well) in 24-well plates in triplicate. Cells are counted using a Coulter Particle Counter. Oleic acid-albumin is added to media at 5μMol. A939572 stocks are prepared in DMSO. Temsirolimus dosing is performed. Soft agar cultures are prepared by diluting 2× growth medium 1:1 in 1.5% Seaplaque^®GTG^® agarose, with 500 cells/plate in 60mm culture dishes. Colonies are stained with Giemsa and counted after 3wks^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Mice^[2]
A498 cells are subcutaneously implanted in athymic nu/nu mice at 1×10⁶ cells/mouse in 50% Matrigel. Tumors reach ~50 mm³ prior to 4 wk treatment. A939572 is re-suspended in strawberry flavored Kool-Aid^® in sterilized H₂O (0.2 g/mL) vehicle at 30 mg/kg in a 50 μL dose. Mice are orally fed by using a syringe to administer the 50 μL dose twice daily/mouse. This modified method is found to be effective and less stressful on the mice. Temsirolimus is solubilized in 30% ethanol/saline and administered via intraperitoneal injection at 10 mg/kg in a 50 μL dose once every 72 hrs/mouse. Tumor volumes are calculated using the formula 0.5236 (L*W*H) and body weight are measured every 3 days.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Xin Z, et al. Discovery of piperidine-aryl urea-based stearoyl-CoA desaturase 1 inhibitors. Bioorg Med Chem Lett. 2008 Aug 1;18(15):4298-302. [Content Brief]

[2]. von Roemeling CA, et al. Stearoyl-CoA desaturase 1 is a novel molecular therapeutic target for clear cell renal cell carcinoma. Clin Cancer Res. 2013 May 1;19(9):2368-80. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5782 mL	12.8912 mL	25.7825 mL	64.4562 mL
	5 mM	0.5156 mL	2.5782 mL	5.1565 mL	12.8912 mL
	10 mM	0.2578 mL	1.2891 mL	2.5782 mL	6.4456 mL
	15 mM	0.1719 mL	0.8594 mL	1.7188 mL	4.2971 mL
	20 mM	0.1289 mL	0.6446 mL	1.2891 mL	3.2228 mL
	25 mM	0.1031 mL	0.5156 mL	1.0313 mL	2.5782 mL
	30 mM	0.0859 mL	0.4297 mL	0.8594 mL	2.1485 mL
	40 mM	0.0645 mL	0.3223 mL	0.6446 mL	1.6114 mL
	50 mM	0.0516 mL	0.2578 mL	0.5156 mL	1.2891 mL
	60 mM	0.0430 mL	0.2149 mL	0.4297 mL	1.0743 mL
	80 mM	0.0322 mL	0.1611 mL	0.3223 mL	0.8057 mL
	100 mM	0.0258 mL	0.1289 mL	0.2578 mL	0.6446 mL

Keywords

A939572

41 Cited Publications

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A939572

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Endoplasmic Reticulum Stress Compound Library

Orally Active Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Lipid Metabolism Compound Library

Human Metabolite Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Publications Citing Use of MedChemExpress (MCE) A939572

Biologische Aktivität

Chemical Information

Publications (41)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

A939572

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Endoplasmic Reticulum Stress Compound Library

Orally Active Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Lipid Metabolism Compound Library

Human Metabolite Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Publications Citing Use of MedChemExpress (MCE) A939572

Biologische Aktivität

Chemical Information

Publications (41)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

A939572 Related Classifications

HY-50709 Related Classifications