Art. -Nr.: HY-19330

DASA-58

Name: DASA-58
Brand: MedChemExpress
SKU: HY-19330
Price: 80.00 USD
Availability: InStock
Rating: 5 (19 reviews)

19 Cited Publications

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Based on 19 publication(s) in Google Scholar

DASA-58 is a potential pyruvate kinase isozyme (PKM2) allosteric activator. DASA-58 can be used for the research of metabolism and kinds of cancer.

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Reinheit : 99.57%
CAS. Nr.: 1203494-49-8
Formel: C₁₉H₂₃N₃O₆S₂
Molecular Weight:453.53

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

DASA-58 Chemical Structure

CAS. Nr.: 1203494-49-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (19)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

DASA-58 is a potential pyruvate kinase isozyme (PKM2) allosteric activator. DASA-58 can be used for the research of metabolism and kinds of cancer^[1].

In Vitro

DASA-58 (15 μM; 2 h) potentiates the antitumor effects of other metabolic stressors^[1].
DASA-58 (15 μM; 24 h, 72 h) enhances pyruvate kinase activity in breast cancer cells without a clear effect on proliferation^[1].
DASA-58 (30 μM, 60 μM; 0-72 h) enhances extracellular acidification and lactate levels in BCa cell lines and induce extracellular acidification levels in prostate cancer cell lines^[1].
DASA-58 (15 μM, 30 μM; 0-72 h) affects respiration levels in BCa cells without an indication of mitochondrial damage^[1].
DASA-58 (15 μM; 0-72 h) not rescues TXNIP levels in any combintion and mitochondrial inhibitors enhance PKM2 effects on activating AMPK signaling (T172 phosphorylation of AMPK) ^[1].
DASA-58 (15 μM; 0-72 h) leads to depletion in TXNIP levels independent of AMPK and ER signaling, and not through enhanced proteasomal degradation but rather depleted upstream glycolytic intermediates^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Five breast cancer cell lines (BCa cells)
(MDA MB 231, MDA MB 468, HCC 1443, T47-D, MCF7, LnCap, PC3, and DU145)
Concentration:

15 μM
Incubation Time:

2 h
Result:

Could be exploited by other metabolic stressors.

Western Blot Analysis^[1]

Cell Line:

BCa cells
Concentration:

15 μM
Incubation Time:

24 h, 72 h
Result:

Showed comparable PKM2 protein levels in five breast cancer cell lines, except HCC1443 cells and MDA MB 468 that showed the highest and lowest PKM2 protein levels, respectively.
Not changed PKM2 levels in five breast cancer cell lines but seemingly reduced TXNIP levels in cells expressing detectable TXNIP levels.

Chemical Information

CAS. Nr. 1203494-49-8
Appearance Solid
Molecular Weight 453.53
Formel C₁₉H₂₃N₃O₆S₂
Color White to off-white
SMILES

NC1=CC=CC(S(=O)(N2CCN(S(=O)(C3=CC=C(OCCO4)C4=C3)=O)CCC2)=O)=C1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (19)

All Bio/Physico-chemical AssayCell Proliferation/Viability AssayCell Imaging/StainingWB

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Journal Impact Factor

Nature

Dietary fructose improves intestinal cell survival and nutrient absorption. [Abstract]

2021 Sep;597(7875):263-267. PMID: 34408323
Nat Immunol

The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers. [Abstract]

2018 Mar;19(3):267-278. PMID: 29358707

DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2018 Mar;19(3):267-278. [Abstract]

CellTrace Violet profile from in vitro stimulated splenic B cells stimulated for 62 hours as indicated above the histogram overlays in the presence of DASA-58 (12.5 μM) or vehicle control (as the highest concentration of DASA-58).

DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2018 Mar;19(3):267-278. [Abstract]

DASA-58 (12.5 μM) reduced the proliferation of mouse B cells in response to in vitro stimulation with anti-CD40+IL-4+IL-5 or anti-IgM+IL-4.
Nat Aging

The glycolytic metabolite phosphoenolpyruvate restricts cGAS-driven inflammation to promote healthy aging. [Abstract]

2026 Apr;6(4):831-848. PMID: 41792330
Cancer Res

Pharmacologic Targeting of TFIIH Suppresses KRAS-Mutant Pancreatic Ductal Adenocarcinoma and Synergizes with TRAIL. [Abstract]

2022 Sep 16;82(18):3375-3393. PMID: 35819261
Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. [Abstract]

2020 Jun 22;11(1):3162. PMID: 32572027

DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jun 22;11(1):3162. [Abstract]

The glycolysis analysis of HepG2 cells overexpressing HULC treated by FGFR1 inhibitor PD166866 (2.5 μM), PKM2 activator DASA-58 (30 μM, 0-100 min), or LDHA inhibitor GSK2837808A (10 μM). DMSO was added instead in the control groups.

DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jun 22;11(1):3162. [Abstract]

Proliferation of HepG2 cells with HULC knockdown (left panel) or overexpression (right panel) was measured with a CCK8 assay. For rescue experiments, HepG2-HULC cells were treated by FGFR1 inhibitor PD 166866 (2.5 μM), PKM2 activator DASA-58 (30 μM), or LDHA inhibitor GSK2837808A (10 μM), respectively.
Nat Commun

Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection. [Abstract]

2020 Feb 18;11(1):941. PMID: 32071304

DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]

Primary astrocytes were pretreated with 50 µM DASA-58 for 1 h and then stimulated with 10 µM quinpirole for 12 h. PKM2 and Nrf2 proximity ligation signals.

DASA-58 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Feb 18;11(1):941. [Abstract]

Primary astrocytes were pretreated with 50 µM DASA-58 for 1 h and then stimulated with 10 µM quinpirole for 12 h. DASA-58 treatment attenuated DRD2 activation-induced PKM2 dimerization.
Redox Biol

Activation of sclerostin inhibits Isg20-Mediated aerobic glycolysis ameliorating renal Fibrosis: the renoprotective mechanism of hederagenin in CKD. [Abstract]

2025 Sep:85:103762. PMID: 40644925
Adv Sci (Weinh)

F13A1-Mediated Macrophage Activation Promotes MASH Progression via the PKM2/HIF1A Pathway. [Abstract]

2025 Dec 19:e18128. PMID: 41417477
Sci Signal

Modulation of PKM activity affects the differentiation of TH17 cells. [Abstract]

2020 Oct 27;13(655):eaay9217. PMID: 33109748
J Agric Food Chem

Tetrahydroxy Stilbene Glucoside Alleviates Ischemic Stroke by Regulating Conformation-Dependent Intracellular Distribution of PKM2 for M2 Macrophage Polarization. [Abstract]

2022 Dec 14;70(49):15449-15463. PMID: 36468551
Ecotoxicol Environ Saf

MC-LR triggers trophoblast ferroptosis at the maternal-fetal interface by directly targeting PKM2 to reprogram metabolism. [Abstract]

2025 Nov 15:307:119451. PMID: 41273830
Drug Des Devel Ther

Dihydroartemisinin Inhibits the Proliferation of Leukemia Cells K562 by Suppressing PKM2 and GLUT1 Mediated Aerobic Glycolysis. [Abstract]

2020 May 27:14:2091-2100. PMID: 32546972

DASA-58 purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2020 May 27:14:2091-2100. [Abstract]

The growth inhibition of DHA after 24 hrs together with 40 μM DASA-58 (a PKM2 agonist). Tumor cell growth inhibition is determined by CCK-8 after treated with various concentrations of DHA at different times.
Int Immunopharmacol

2026 Oct 1:186:117062. PMID: 42385640
Mucosal Immunol

Intestinal epithelial PKM2 serves as a safeguard against experimental colitis via activating β-catenin signaling. [Abstract]

2019 Nov;12(6):1280-1290. PMID: 31462699
Am J Pathol

Pyruvate Kinase M2 Tetramerization Protects against Hepatic Stellate Cell Activation and Liver Fibrosis. [Abstract]

2020 Nov;190(11):2267-2281. PMID: 32805235
Arch Biochem Biophys

Chaetocin attenuates gout in mice through inhibiting HIF-1α and NLRP3 inflammasome-dependent IL-1β secretion in macrophages. [Abstract]

2019 Jul 30:670:94-103. PMID: 31255694
Animals (Basel)

Glycolysis Combined with Core Pluripotency Factors to Promote the Formation of Chicken Induced Pluripotent Stem Cells. [Abstract]

2021 Feb 6;11(2):425. PMID: 33562170
Oncol Lett

Interference with ENO2 promotes ferroptosis and inhibits glycolysis in clear cell renal cell carcinoma by regulating Hippo‑YAP1 signaling. [Abstract]

2024 Jul 19;28(3):443. PMID: 39091581
bioRxiv

2026 Jan 20.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (275.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2049 mL

11.0246 mL

22.0493 mL

5 mM

0.4410 mL

2.2049 mL

4.4099 mL

10 mM

0.2205 mL

1.1025 mL

2.2049 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Fadi Almouhanna, et al. Pharmacological activation of pyruvate kinase M2 reprograms glycolysis leading to TXNIP depletion and AMPK activation in breast cancer cells. Cancer Metab. 2021 Jan 22;9(1):5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2049 mL	11.0246 mL	22.0493 mL	55.1231 mL
	5 mM	0.4410 mL	2.2049 mL	4.4099 mL	11.0246 mL
	10 mM	0.2205 mL	1.1025 mL	2.2049 mL	5.5123 mL
	15 mM	0.1470 mL	0.7350 mL	1.4700 mL	3.6749 mL
	20 mM	0.1102 mL	0.5512 mL	1.1025 mL	2.7562 mL
	25 mM	0.0882 mL	0.4410 mL	0.8820 mL	2.2049 mL
	30 mM	0.0735 mL	0.3675 mL	0.7350 mL	1.8374 mL
	40 mM	0.0551 mL	0.2756 mL	0.5512 mL	1.3781 mL
	50 mM	0.0441 mL	0.2205 mL	0.4410 mL	1.1025 mL
	60 mM	0.0367 mL	0.1837 mL	0.3675 mL	0.9187 mL
	80 mM	0.0276 mL	0.1378 mL	0.2756 mL	0.6890 mL
	100 mM	0.0220 mL	0.1102 mL	0.2205 mL	0.5512 mL

Bioz

Keywords

DASA-58

19 Cited Publications

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DASA-58

Publications Citing Use of MedChemExpress (MCE) DASA-58