Art. -Nr.: HY-15945

DBeQ

(Synonyms: JRF 12)

8 Cited Publications

Kundenbewertung

Based on 8 publication(s) in Google Scholar

DBeQ is a selective, potent, reversible, and ATP-competitive p97 inhibitor, with an IC₅₀ value of 1.5 μM and 1.6 μM for p97(wt) and p97(C522A), respectively; DBeQ also inhibits Vps4 with an IC₅₀ of 11.5 μM.

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Reinheit: 98.79%
CAS. Nr.: 177355-84-9
Formel: C₂₂H₂₀N₄
Molecular Weight:340.42

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

DBeQ Chemical Structure

CAS. Nr.: 177355-84-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (8)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 1.5 μM (p97)^[1], 11.5 μM (Vps4)^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	IC₅₀	4.72 μM Compound: 3e	Cytotoxicity against human HT-29 cells after 72 hrs by neutral red dye assay Cytotoxicity against human HT-29 cells after 72 hrs by neutral red dye assay	[PMID: 21700369]
HT-29	IC₅₀	4.72 μM Compound: I	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis	[PMID: 35694693]
J774.A1	EC₅₀	0.67 μM Compound: 10	Antileishmanial activity against intracellular amastigote stage of Leishmania donovani MHOM/ET/67/L82 infected in mouse J774A1 cells by colorimetric assay Antileishmanial activity against intracellular amastigote stage of Leishmania donovani MHOM/ET/67/L82 infected in mouse J774A1 cells by colorimetric assay	[PMID: 24874647]
J774.A1	EC₅₀	1.4 μM Compound: 10	Antileishmanial activity against intracellular amastigote stage of Leishmania amazonensis LV78 infected in mouse J774A1 cells by colorimetric assay Antileishmanial activity against intracellular amastigote stage of Leishmania amazonensis LV78 infected in mouse J774A1 cells by colorimetric assay	[PMID: 24874647]
J774.A1	EC₅₀	5.5 μM Compound: 1	Cytotoxicity against mouse J774A.1 cells assessed as inhibition of cell viability after 72 hrs by MTT assay Cytotoxicity against mouse J774A.1 cells assessed as inhibition of cell viability after 72 hrs by MTT assay	[PMID: 25749014]
J774.A1	EC₅₀	5.5 μM Compound: 10	Cytotoxicity against mouse J774A1 cells after 72 hrs by MTT assay Cytotoxicity against mouse J774A1 cells after 72 hrs by MTT assay	[PMID: 24874647]
MDA-MB-231	IC₅₀	1.79 μM Compound: 3e	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by neutral red dye assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by neutral red dye assay	[PMID: 21700369]
MDA-MB-231	IC₅₀	1.79 μM Compound: I	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis	[PMID: 35694693]
T-24	IC₅₀	5.96 μM Compound: 3e	Cytotoxicity against human T24 cells after 72 hrs by neutral red dye assay Cytotoxicity against human T24 cells after 72 hrs by neutral red dye assay	[PMID: 21700369]
T-24	IC₅₀	5.96 μM Compound: I	Antiproliferative activity against human T24 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis Antiproliferative activity against human T24 cells assessed as inhibition of cell growth incubated for 72 hrs by neutral red dye based spectrophotometric analysis	[PMID: 35694693]

In Vitro

DBeQ is a ATP-competitive p97 inhibitor, with an IC₅₀ value of 1.5 μM and 1.6 μM for p97(wt) and p97(C522A), respectively. DBeQ inhibits p97 competitively with respect to ATP, with a K_i of 3.2 ± 0.4 μM. DBeQ inhibits degradation of the p97-dependent substrate UbG76V-GFP, with IC₅₀ value of 2.6 μM. DBeQ (10 μM) also significantly suppresses degradation of TCRα-GFP, induces CHOP but does not increase p21 level. Moreover, DBeQ inhibits the viability of MRC-5, Hek293, HeLa and RPMI8226 cells, with GI₅₀s of 6.6 ± 2.9, 4 ± 0.6, 3.1 ± 0.5 and 1.2 ± 0.3, respectively^[1]. DBeQ potently inhibits the AAA ATPase p97 by specifically binding to the ATPase site of its D2 domain (p97D2). DBeQ also inhibits Vps4, with an IC₅₀ of 11.5 μM. Furthermore, DBeQ (30 μM) inhibits hyphal growth of the wild-type cell (strain YLZ0)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 177355-84-9
Appearance Solid
Molecular Weight 340.42
Formel C₂₂H₂₀N₄
Color White to off-white
SMILES

C1(NCC2=CC=CC=C2)=NC(NCC3=CC=CC=C3)=C4C=CC=CC4=N1
Synonyms

JRF 12
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

All WBIFRT-PCRBio/Physico-chemical AssayCell Imaging/Staining

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Journal Impact Factor

Nat Commun

Coronaviral nsp6 hijacks ERAD machinery to facilitate lipolysis and supply membrane components for DMV growth. [Abstract]

2025 Nov 18;16(1):10094. PMID: 41253842

DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]

We examined the turnover of PLIN2 controlled by nsp6 and observed that nsp6 increased the degradation of PLIN2 protein, while the treatment of MG132 or the ERAD inhibitor DBeQ blocked nsp6-mediated reduction in PLIN2 protein level; CQ was used as a negative control.

DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]

Confocal microscopy of Huh-7 cells transfected with mCherry-nsp6 for 24 h and then treated with 5 μM DBeQ for an additional 12 h.

DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]

HeLa-mCEACAM1 cells were infected with MHV-A59 and treated with DBeQ (0-5 μM). The cell lysates were subjected to qRT-PCR.

DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]

HeLa-mCEACAM1 cells were infected with MHV-A59 and treated with DBeQ (0-5 μM, 12-24 h). The cell lysates were subjected to immunoblot analysis.

DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]

HeLa-mCEACAM1 cells were infected with MHV-A59 and treated with DBeQ (0-5 μM). The supernatant was used for plaque assay.

DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]

17Cl-1 cells were pre-treated with 5 μM DBeQ overnight and then infected with MHV-A59 at MOI of 5 for 12 h.

DBeQ purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10094. [Abstract]

17Cl-1 cells were infected with MHV at MOI of 5 for 2 h and treated with 5 μM DBeQ for 10 h.
Nat Commun

Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling. [Abstract]

2025 Oct 29;16(1):9554. PMID: 41162367
Autophagy

Pestivirus bovine viral diarrhea virus infection triggers lipophagy through the AMPK-PNPLA2/ATGL signaling pathway to promote viral replication. [Abstract]

2025 Aug 18:1-20. PMID: 40790888
Inflammation

VCP Inhibition Augments NLRP3 Inflammasome Activation. [Abstract]

2024 Oct;47(5):1868-1883. PMID: 38563877
PLoS Pathog

The protein segregase VCP/p97 promotes host antifungal defense via regulation of SYK activation. [Abstract]

2024 Oct 29;20(10):e1012674. PMID: 39471181
Free Radic Res

4-HNE induces cell death through the VCP-related ubiquitination pathway in diabetic cardiomyopathy and the protective effect of metformin. [Abstract]

2026 Mar;60(3):270-288. PMID: 42033421
Discov Oncol

CircHIPK3 negatively regulates autophagy by blocking VCP binding to the Beclin 1 complex in bladder cancer. [Abstract]

2023 Jun 3;14(1):86. PMID: 37269429
Vet Microbiol

Valosin-containing protein (VCP/p97) is responsible for the endocytotic trafficking of classical swine fever virus. [Abstract]

2022 Sep:272:109511. PMID: 35849988

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 35.71 mg/mL (104.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9375 mL

14.6877 mL

29.3755 mL

5 mM

0.5875 mL

2.9375 mL

5.8751 mL

10 mM

0.2938 mL

1.4688 mL

2.9375 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[1]

Cells are seeded on a 384-well solid white plate (5,000 cells/well). Cells are transfected with luciferase siRNA or p97 siRNA (10 nM) for 48 h or treated with DBeQ for the indicated amount of time. Caspase-3/7 Glo, caspase-6 Glo, caspase-8 Glo, or caspase-9 Glo is added into each well and mixed by shaking at 500 rpm for 1 min. Luminescence signal is determined after incubation at room temperature for 1 h. Cellular viability is determined with CellTiter-Glo reagent. To determine the IC₅₀ of cellular viability, cells are treated with MG132 or DBeQ at seven concentrations (threefold serial dilutions starting at 33 μM) for 48 h. IC₅₀ values are calculated from fitting the percentage of luminescence signal normalized to DMSO treated cells)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Chou TF, et al.Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways. Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4834-9. [Content Brief]

[2]. Zhang Y, et al. The AAA ATPase Vps4 Plays Important Roles in Candida albicans Hyphal Formation and is Inhibited by DBeQ. Mycopathologia. 2016 Jun;181(5-6):329-39. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9375 mL	14.6877 mL	29.3755 mL	73.4387 mL
	5 mM	0.5875 mL	2.9375 mL	5.8751 mL	14.6877 mL
	10 mM	0.2938 mL	1.4688 mL	2.9375 mL	7.3439 mL
	15 mM	0.1958 mL	0.9792 mL	1.9584 mL	4.8959 mL
	20 mM	0.1469 mL	0.7344 mL	1.4688 mL	3.6719 mL
	25 mM	0.1175 mL	0.5875 mL	1.1750 mL	2.9375 mL
	30 mM	0.0979 mL	0.4896 mL	0.9792 mL	2.4480 mL
	40 mM	0.0734 mL	0.3672 mL	0.7344 mL	1.8360 mL
	50 mM	0.0588 mL	0.2938 mL	0.5875 mL	1.4688 mL
	60 mM	0.0490 mL	0.2448 mL	0.4896 mL	1.2240 mL
	80 mM	0.0367 mL	0.1836 mL	0.3672 mL	0.9180 mL
	100 mM	0.0294 mL	0.1469 mL	0.2938 mL	0.7344 mL

Keywords

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DBeQ

Anti-Infection Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Ubiquitination Compound Library

Endoplasmic Reticulum Stress Compound Library

Exosomes Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) DBeQ

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DBeQ

Anti-Infection Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Ubiquitination Compound Library

Endoplasmic Reticulum Stress Compound Library

Exosomes Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) DBeQ

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DBeQ Related Classifications

HY-15945 Related Classifications