Art. -Nr.: HY-Y0016

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Rhodamine B

(Synonyms: Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine)

24 Cited Publications

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Based on 24 publication(s) in Google Scholar

Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.

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Reinheit: 98.94%
CAS. Nr.: 81-88-9
Formel: C₂₈H₃₁ClN₂O₃
Molecular Weight:479.01

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Rhodamine B Chemical Structure

CAS. Nr.: 81-88-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- Emission (Em)
- Excitation (Ex)
- Dyeing Example
Chemical Information
Publications (24)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.

Cellular Effect

Cell Line	Type	Value	Description	References
518A2	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay	[PMID: 28033541]
8505C	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human 8505C cells after 96 hrs by SRB assay Cytotoxicity against human 8505C cells after 96 hrs by SRB assay	[PMID: 28033541]
A2780	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human A2780 cells after 96 hrs by SRB assay Cytotoxicity against human A2780 cells after 96 hrs by SRB assay	[PMID: 28033541]
A2780	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
A2780	EC₅₀	>30 μM Compound: 5	Cytotoxicity against human A2780 cells incubated for 72 hrs by SRB assay Cytotoxicity against human A2780 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
A-375	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
A-375	EC₅₀	>30 μM Compound: 5	Cytotoxicity against human A-375 cells incubated for 72 hrs by SRB assay Cytotoxicity against human A-375 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
A549	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human A549 cells after 96 hrs by SRB assay Cytotoxicity against human A549 cells after 96 hrs by SRB assay	[PMID: 28033541]
FaDu	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
HEK293	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
HT-29	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay	[PMID: 28033541]
HT-29	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
HT-29	EC₅₀	>30 μM Compound: 5	Cytotoxicity against human HT-29 cells incubated for 72 hrs by SRB assay Cytotoxicity against human HT-29 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
MCF7	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay	[PMID: 28033541]
MCF7	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
MCF7	EC₅₀	>30 μM Compound: 5	Cytotoxicity against human MCF7 cells incubated for 72 hrs by SRB assay Cytotoxicity against human MCF7 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]
NIH3T3	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay	[PMID: 28033541]
NIH3T3	EC₅₀	>30 μM Compound: Rhodamine B	Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay	[PMID: 33049606]
NIH3T3	EC₅₀	>30 μM Compound: 5	Cytotoxicity against mouse NIH/3T3 cells incubated for 72 hrs by SRB assay Cytotoxicity against mouse NIH/3T3 cells incubated for 72 hrs by SRB assay	[PMID: 36780832]

In Vitro

Rhodamine B induces a concentration-dependent reduction of root meristem cells of A. cepa. mitotic activity. Rhodamine B induces various nuclear aberrations in A. cepa. root cells. In the 100 and 200 ppm rhodamine B groups, the frequencies of NBUDs and BN surpass those of the positive control (MMS) group. Rhodamine B-induced changes of H₂O₂ (a) and MDA (b) level increase in a concentration-dependent manner in A. cepa. roots^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

610

Excitation (Ex)

546

Dyeing Example

Description: Rhodamine B is a red fluorescent dye, it can be used to detect the biocatalytic membranes.
Method: For laccase stamp.

Chemical Information

CAS. Nr. 81-88-9
Appearance Solid
Molecular Weight 479.01
Formel C₂₈H₃₁ClN₂O₃
Color Dark purple to black
SMILES

CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=CC=CC=C4C(O)=O)=C2C=C1)CC.[Cl-]
Synonyms

Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Publications (24)

All In Vivo ImagingOthersCell Imaging/Staining

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Journal Impact Factor

Adv Mater

Disrupting the Oxidative Stress-Inflammation-Corneal Neovascularization Cycle with Ultrasmall Nanoparticles. [Abstract]

2026 Apr 24:e73114. PMID: 42032871
ACS Nano

Osteoinductive Dental Pulp Stem Cell-Derived Extracellular Vesicle-Loaded Multifunctional Hydrogel for Bone Regeneration. [Abstract]

2024 Mar 26;18(12):8777-8797. PMID: 38488479

Rhodamine B purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Mar 26;18(12):8777-8797. [Abstract]

Fluorescence images showing the retention of Rhodamine B-labeled Ost-EVs after intramuscular injection. Before experiments, Ost-EVs were incubated with 10 μM Rhodamine B for 2 h and ultrafiltrated (10 kDa) at 14 000 × g for 15 min. Then, the Ost-EV-laden hydrogels were intramuscularly injected into the muscles of the rat’s hind limb. Ost-EVs in equivalent PBS were used as controls.
Nat Commun

Interaction-aware dexterous robot for minimally invasive transcanal inner ear interventions. [Abstract]

2026 Apr 24;17(1):5658. PMID: 42031753
Chem Eng J

2020, 127870.
Biomaterials

Reversing neuroinflammation in ischemic stroke through efferocytotic phenotype reprogramming with polymeric nanoparticles. [Abstract]

2026 Sep:332:124156. PMID: 41895020
Biomaterials

Radiation-triggerable bioreactors enable bioenergetic reprograming of cancer stem cell plasticity via targeted arginine metabolism disruption for augmented radio-immunotherapy. [Abstract]

2025 May 5:322:123391. PMID: 40344881

Rhodamine B purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 May 5:322:123391. [Abstract]

Design and construction of RTBs for remodeling CSC plasticity. Fluorescence emission spectra of FITC-RTB-RhB at 488 nm. (FITC: HY-66019; RhB, Rhodamine B, HY-Y0016)

Rhodamine B purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 May 5:322:123391. [Abstract]

CLSM images showing the time-dependent changes in the intracellular localization of RhB (Rhodamine B)-labeled pDNAiNOS after incubating Hep1-6-derived HCSCs with RTB (pDNAiNOS-RhB), lysosomes were labeled green with Lyso-TrackerGreen, and cell nuclei were labeled blue with Hoechst.

Rhodamine B purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 May 5:322:123391. [Abstract]

CLSM analysis on the subcellular co-localization of RhB (Rhodamine B)-labeled pDNAiNOS and Nfr2 after incubating Hep1-6-derived HCSCs with pDNAiNOS-RhB-based RTBs with or without LDR.
Biomaterials

Single-dose of integrated bilayer microneedles for enhanced hypertrophic scar therapy with rapid anti-inflammatory and sustained inhibition of myofibroblasts. [Abstract]

2025 Jan:312:122742. PMID: 39106821
J Nanobiotechnology

Oral alginate microspheres deliver Rg3/aspirin liposomes to modulate foam cells and gut microbiota in atherosclerosis. [Abstract]

2026 May 3;24(1):586. PMID: 42071219
J Nanobiotechnology

Dual-function MgSrCeAl-LDH nanosheets enable both ROS clearance and enhanced angiogenesis for myocardial ischemia/reperfusion injury therapy. [Abstract]

2025 Oct 27;23(1):690. PMID: 41139778
J Nanobiotechnology

Overcoming acquired immunotherapy resistance in non-small cell lung cancer using ginsenoside Rb1-loaded, peptide-enhanced exosome delivery systems. [Abstract]

2025 Jun 13;23(1):443. PMID: 40514658

Rhodamine B purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Jun 13;23(1):443. [Abstract]

Colocalization of PKH-67-exo and Rhd-TMTP1 observed under a confocal laser scanning microscope (white arrows indicate colocalized PKH-67-exo and Rhd-TMTP1, scale bar = 15 μm). Rhd (Rhodamine), HY-Y0016; PKH-67, HY-D1421.
J Control Release

Dual-action endoplasmic reticulum membrane-fusogenic immunogenic liposomes amplify cellular immunity in anti-tumor vaccine design. [Abstract]

2026 Jul 10:395:114937. PMID: 41999902
Mater Today Bio

Mannosylated graphene oxide nanotherapeutics co-delivering docetaxel and a STING agonist reprogram myeloid cells and potentiate antitumor immunity. [Abstract]

2026 Mar 31:38:103086. PMID: 42006712
Adv Healthc Mater

Triple-Tailored Analgesic Hydrogel System Targeting ADAM17 in Orofacial Inflammatory Pain. [Abstract]

2025 Jul 16:e2501440. PMID: 40671384
Int J Biol Macromol

Advancing vaccine development: Evaluation of a mannose-modified lipid nanoparticle-based candidate for African swine fever p30 mRNA vaccine eliciting robust immune response in mice. [Abstract]

2024 May 17;270(Pt 1):132432. PMID: 38761609
EMBO J

SIRT7 activates quiescent hair follicle stem cells to ensure hair growth in mice. [Abstract]

2020 Sep 15;39(18):e104365. PMID: 32696520
Int J Nanomedicine

pH-Responsive Mesoporous Silica Nanoparticles Loaded with Naringin for Targeted Osteoclast Inhibition and Bone Regeneration. [Abstract]

2024 Jun 24:19:6337-6358. PMID: 38946884
ACS Appl Bio Mater

PEGylated Graphene Oxide as a Nanocarrier Assists Alpha Lipoic Acid Mitigating Hypoxia Induced Mitochondrial Dysfunction. [Abstract]

2026 Feb 2;9(3):1352-1364. PMID: 41535114
Nanomedicine

Precise atorvastatin delivery by cardiac homing peptide functionalized nanoliposomes for myocardial damage repair after myocardial infarction. [Abstract]

2026 Mar;21(6):789-801. PMID: 41660754
Biomed J

Targeted dexamethasone nano-prodrug for corneal neovascularization management. [Abstract]

2024 Feb;47(1):100592. PMID: 37004870
ACS Omega

Screening the Degradation of Polymer Microparticles on a Chip. [Abstract]

2022 Dec 23;8(1):1710-1722. PMID: 36643556
ACS Synth Biol

Signal Peptide-Guided Delivery of a Mucin-Like Collagen Analogue for Periplasmic Barrier Reinforcement: A Platform for Enhancing Microbial Survival. [Abstract]

2025 Sep 19;14(9):3767-3783. PMID: 40924575
PLoS Genet

A novel role of tRNA-derived fragments in porcine granulosa-oocyte cell communication and cuproptosis. [Abstract]

2026 Apr 30;22(4):e1012119. PMID: 42060579
Biomed Res Int

Multifunctional Drug-Loaded Phase-Change Nanoparticles Inhibit the Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma by Affecting the Activity of Activated Hepatic Stellate Cells. [Abstract]

2022 Nov 12:2022:6441179. PMID: 36411770
Biochim Biophys Acta Gen Subj

Unraveling the power of TOMM40: Driving PHB1-mediated mtDNA release and mitophagy to fuel breast cancer progression. [Abstract]

2025 Aug 13;1869(11):130851. PMID: 40816542

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (260.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (208.76 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0876 mL

10.4382 mL

20.8764 mL

5 mM

0.4175 mL

2.0876 mL

4.1753 mL

10 mM

0.2088 mL

1.0438 mL

2.0876 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Tan D, et al. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells. Environ Sci Pollut Res Int. 2014 Mar;21(5):3363-70. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.0876 mL	10.4382 mL	20.8764 mL	52.1910 mL
	5 mM	0.4175 mL	2.0876 mL	4.1753 mL	10.4382 mL
	10 mM	0.2088 mL	1.0438 mL	2.0876 mL	5.2191 mL
	15 mM	0.1392 mL	0.6959 mL	1.3918 mL	3.4794 mL
	20 mM	0.1044 mL	0.5219 mL	1.0438 mL	2.6095 mL
	25 mM	0.0835 mL	0.4175 mL	0.8351 mL	2.0876 mL
	30 mM	0.0696 mL	0.3479 mL	0.6959 mL	1.7397 mL
	40 mM	0.0522 mL	0.2610 mL	0.5219 mL	1.3048 mL
	50 mM	0.0418 mL	0.2088 mL	0.4175 mL	1.0438 mL
	60 mM	0.0348 mL	0.1740 mL	0.3479 mL	0.8698 mL
	80 mM	0.0261 mL	0.1305 mL	0.2610 mL	0.6524 mL
	100 mM	0.0209 mL	0.1044 mL	0.2088 mL	0.5219 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Rhodamine B

24 Cited Publications

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Rhodamine B

Publications Citing Use of MedChemExpress (MCE) Rhodamine B

Biologische Aktivität

Chemical Information

Publications (24)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Rhodamine B Related Classifications

HY-Y0016 Related Classifications