Art. -Nr.: HY-100224

SANT-1

4 Cited Publications

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Based on 4 publication(s) in Google Scholar

SANT-1, a potent Smo antagonist, inhibits Hedgehog signaling. SANT-1 shows IC₅₀s of 20 nM and 30 nM in Shh-LIGHT2 and SmoA1-LIGHT2 assay, respectively.

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Reinheit: 99.81%
CAS. Nr.: 304909-07-7
Formel: C₂₃H₂₇N₅
Molecular Weight:373.49

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

SANT-1 Chemical Structure

CAS. Nr.: 304909-07-7

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

SANT-1, a potent Smo antagonist, inhibits Hedgehog signaling. SANT-1 shows IC₅₀s of 20 nM and 30 nM in Shh-LIGHT2 and SmoA1-LIGHT2 assay, respectively^[1].

IC₅₀ & Target

IC50: 20 nM (Shh signaling), 30 nM (SmoA1)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
C3H 10T1/2	IC₅₀	20 nM Compound: 50, SANT-1	Inhibition of SHH-mediated mouse C3H10T1/2 cells differentiation into osteoblasts by alkaline phosphatase assay Inhibition of SHH-mediated mouse C3H10T1/2 cells differentiation into osteoblasts by alkaline phosphatase assay	[PMID: 19309080]
HEK293	IC₅₀	25 nM Compound: 4, SANT-1	Inhibition of Smoothened transfected in HEK293 cells by binding assay Inhibition of Smoothened transfected in HEK293 cells by binding assay	[PMID: 20620058]
HEK293	IC₅₀	4.74 nM Compound: 4, SANT-1	Inhibition of Smoothened transfected in HEK293 cells by transient transfection cell-based assay Inhibition of Smoothened transfected in HEK293 cells by transient transfection cell-based assay	[PMID: 20620058]

In Vitro

SANT-1 is a potent Smo antagonist, binds directly to Smo, and inhibits Hedgehog signaling, with IC₅₀s of 20 nM and 30 nM in Shh-LIGHT2 and SmoA1-LIGHT2 cells, respectively^[1].
SANT-1 (1 μM, for 24 or 48 hours) decreases the proliferation of medulloblastoma cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

Medulloblastoma cells
Concentration:

1 μM
Incubation Time:

24 hours or 48 hours
Result:

Inhibited cellular proliferation in primary tumorspheres.

Chemical Information

CAS. Nr. 304909-07-7
Appearance Solid
Molecular Weight 373.49
Formel C₂₃H₂₇N₅
Color White to off-white
SMILES

CC1=C(/C=N/N2CCN(CC3=CC=CC=C3)CC2)C(C)=NN1C4=CC=CC=C4
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

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Journal Impact Factor

Biomed Pharmacother

Interference of sympathetic overactivation restores limbal stem/progenitor cells function and accelerates corneal epithelial wound healing in diabetic mice. [Abstract]

2023 May:161:114523. PMID: 36931034

SANT-1 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May:161:114523. [Abstract]

The promotion effect of ICI on diabetic epithelial regeneration was large partially reversed by Sant-1 (250 nM).

SANT-1 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May:161:114523. [Abstract]

Ki67 staining showed that ICI promoted proliferation in LSPCs were totally reversed by Sant-1 (250 nM).

SANT-1 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May:161:114523. [Abstract]

The elevation effect of ICI on ΔNp63, Krt15 and Gpha2 expression in diabetic wounded corneal limbus were reversed by Sant-1 (250 nM).

SANT-1 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May:161:114523. [Abstract]

The elevation effect of ICI on ΔNp63, Krt15 and Gpha2 expression in diabetic wounded corneal limbus were reversed by Sant-1 (250 nM).
Front Cell Dev Biol

miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells. [Abstract]

2021 May 13:9:673231. PMID: 34055806
Res Sq

2025 Aug 12.
bioRxiv

2024 November 15.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (66.94 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6774 mL

13.3872 mL

26.7745 mL

5 mM

0.5355 mL

2.6774 mL

5.3549 mL

10 mM

0.2677 mL

1.3387 mL

2.6774 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6774 mL	13.3872 mL	26.7745 mL	66.9362 mL
	5 mM	0.5355 mL	2.6774 mL	5.3549 mL	13.3872 mL
	10 mM	0.2677 mL	1.3387 mL	2.6774 mL	6.6936 mL
	15 mM	0.1785 mL	0.8925 mL	1.7850 mL	4.4624 mL
	20 mM	0.1339 mL	0.6694 mL	1.3387 mL	3.3468 mL
	25 mM	0.1071 mL	0.5355 mL	1.0710 mL	2.6774 mL
	30 mM	0.0892 mL	0.4462 mL	0.8925 mL	2.2312 mL
	40 mM	0.0669 mL	0.3347 mL	0.6694 mL	1.6734 mL
	50 mM	0.0535 mL	0.2677 mL	0.5355 mL	1.3387 mL
	60 mM	0.0446 mL	0.2231 mL	0.4462 mL	1.1156 mL

Keywords

SANT-1

4 Cited Publications

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SANT-1

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) SANT-1

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SANT-1

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) SANT-1

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SANT-1 Related Classifications

HY-100224 Related Classifications