Timosaponin AIII
Based on 2 publication(s) in Google Scholar
Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC50 of 35.4 μM.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.86%
- CAS. Nr.: 41059-79-4
- Formel: C39H64O13
- Molecular Weight:740.92
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Publications Citing Use of MedChemExpress (MCE) Timosaponin AIII
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Biologische Aktivität
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AChE |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
5.5 μM
Compound: 1
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Growth inhibition of human HCT116 cells
Growth inhibition of human HCT116 cells
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[PMID: 21370894] |
| HCT-15 | IC50 |
6.1 μM
Compound: 1
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Cytotoxicity against human HCT15 cells after 3 days
Cytotoxicity against human HCT15 cells after 3 days
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[PMID: 21370894] |
| HCT-15 | IC50 |
6.1 μM
Compound: 1
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Growth inhibition of human HCT15 cells
Growth inhibition of human HCT15 cells
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[PMID: 21370894] |
| Hs68 | IC50 |
>50 μM
Compound: 1
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Growth inhibition of human HS68 cells
Growth inhibition of human HS68 cells
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[PMID: 21370894] |
| HT-29 | IC50 |
10.3 μM
Compound: 1
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Growth inhibition of human HT-29 cells
Growth inhibition of human HT-29 cells
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[PMID: 21370894] |
| SW480 | IC50 |
13.1 μM
Compound: 1
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Growth inhibition of human SW480 cells
Growth inhibition of human SW480 cells
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[PMID: 21370894] |
| SW-620 | IC50 |
11.1 μM
Compound: 1
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Growth inhibition of human SW620 cells
Growth inhibition of human SW620 cells
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[PMID: 21370894] |
Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC50 of 35.4 μM[1]. Timosaponin AIII is identified as a major selective cytotoxic activity in BN108, and its selective cytotoxic activity involves inhibition of mTOR, induction of ER stress and protective autophagy[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 41059-79-4
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Appearance Solid
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Molecular Weight 740.92
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Formel C39H64O13
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Color White to off-white
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SMILES
C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@H](C)CO4)([H])C[C@@]1([H])[C@@](CC[C@@]5([H])[C@@]6(CC[C@H](O[C@@](O[C@H](CO)[C@H](O)[C@@H]7O)([H])[C@@H]7O[C@]([C@@H]([C@@H](O)[C@@H]8O)O)([H])O[C@@H]8CO)C5)C)([H])[C@]6([H])CC2
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]2025 May:140:156484. PMID: 40090046 -
Chin Med
Therapeutic potential of Sheng-Xian-Tang in doxorubicin-induced chronic heart failure by regulation of phenylalanine metabolism disruption. [Abstract]2026 Jan 12;21(1):29. PMID: 41527105
Lösungsmittel & Löslichkeit
DMSO : 125 mg/mL (168.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
Mice[1]
Male ICR mice weighing 28-30 g are used. For the acquisition trial, mice are initially placed in the illuminated compartment and the door between the two compartments is opened 10 s later. Each group contains ten mice. One hour or 5 h before the acquisition trial, mice receive each test agent (e.g., Timosaponin AIII 10, 20 or 40 mg/kg, p.o. ). One hour before the acquisition trial, mice receive tacrine (10 mg/kg, p.o.) as a positive control. Memory impairment is induced by scopolamine treatment (1 mg/kg, i.p.) 0.5 h or 4.5 h after the administration of test agents, tacrine, or 10% Tween 80 solution. Control animals are administered 10% Tween 80 solution alone. Twenty-four hours after the acquisition trial, the mice are again placed in the illuminated compartment for retention trials. The time taken for a mouse to enter the dark compartment after the door opened is measured as the latency time in both acquisition and retention trials, with a maximum of 300 s[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Lee B, et al. Timosaponin AIII, a saponin isolated from Anemarrhena asphodeloides, ameliorates learning and memory deficits in mice. Pharmacol Biochem Behav. 2009 Aug;93(2):121-7. [Content Brief]
[2]. King FW, et al. Timosaponin AIII is preferentially cytotoxic to tumor cells through inhibition of mTOR and induction of ER stress. PLoS One. 2009 Sep 30;4(9):e7283. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3497 mL | 6.7484 mL | 13.4967 mL | 33.7418 mL |
| 5 mM | 0.2699 mL | 1.3497 mL | 2.6993 mL | 6.7484 mL | |
| 10 mM | 0.1350 mL | 0.6748 mL | 1.3497 mL | 3.3742 mL | |
| 15 mM | 0.0900 mL | 0.4499 mL | 0.8998 mL | 2.2495 mL | |
| 20 mM | 0.0675 mL | 0.3374 mL | 0.6748 mL | 1.6871 mL | |
| 25 mM | 0.0540 mL | 0.2699 mL | 0.5399 mL | 1.3497 mL | |
| 30 mM | 0.0450 mL | 0.2249 mL | 0.4499 mL | 1.1247 mL | |
| 40 mM | 0.0337 mL | 0.1687 mL | 0.3374 mL | 0.8435 mL | |
| 50 mM | 0.0270 mL | 0.1350 mL | 0.2699 mL | 0.6748 mL | |
| 60 mM | 0.0225 mL | 0.1125 mL | 0.2249 mL | 0.5624 mL | |
| 80 mM | 0.0169 mL | 0.0844 mL | 0.1687 mL | 0.4218 mL | |
| 100 mM | 0.0135 mL | 0.0675 mL | 0.1350 mL | 0.3374 mL |
Timosaponin AIII Related Classifications
HY-N0810 Related Classifications
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