Art. -Nr.: HY-152100

CUR5g

Kundenbewertung

Based on 1 Customer Validation

CUR5g is a potent autophagy inhibitor. CUR5g selectively inhibits autophagosome degradation in cancer cells by blocking autophagosome-lysosome fusion. CUR5g blocks the recruitment of STX17 to autophagosomes via a UVRAG-dependent mechanism, resulting in the inability of autophagosomes to fuse with lysosomes. CUR5g improves the anticancer effect of Cisplatin (HY-17394) against A549 cells both in vitro and in vivo.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 98.69%
CAS. Nr.: 1370032-20-4
Formel: C₂₂H₂₀N₂O₂
Molecular Weight:344.41

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

CUR5g Chemical Structure

CAS. Nr.: 1370032-20-4

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

CUR5g (0-40 μM, 0-24 h) selectively induces autophagosome accumulation in cancer cells^[1].
CUR5g (0-40 μM, 0-24 h) up-regulates LC3B-II and sequestosome 1 (SQSTM1) levels^[1].
CUR5g (0-40 μM, 24 h) inhibits proliferation and migration of A549 cells, but dose not induce apoptosis or necrosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Autophagy Assay^[1]

Cell Line:

A549 cells
Concentration:

0, 1, 5, 10, 20, and 40 μM
Incubation Time:

3, 6, 12, and 24 h
Result:

Induced extensive cytoplasmic vacuolization, and GFP-LC3B signal shifted from diffuse cytosolic staining to a punctate pattern outlining autophagosomes.

Western Blot Analysis^[1]

Cell Line:

A549 cells
Concentration:

0, 1, 5, 10, 20, and 40 μM
Incubation Time:

0, 1, 3, 6, 12, and 24 h
Result:

Up-regulated LC3B-II and sequestosome 1 (SQSTM1) levels time- and dose-dependently. This increase was not the result of enhanced transcription, as mRNA expression of SQSTM1 and LC3B were not increased within CUR5g-exposed cells, suggesting that CUR5g might block autophagic flux rather than increase autophagosome formation.

Cell Proliferation Assay^[1]

Cell Line:

A549 cells
Concentration:

0, 1, 5, 10, 20, and 40 μM
Incubation Time:

24 h
Result:

Exhibited great toxicity to A549 cells at 20 μM. Slightly decreased A549 cell number at 10 µM, while decreased the number of A549 cells significantly at 20 µM. Showed no discernable activity in healthy human umbilical vein endothelial cell (HUVEC) viability at 40 µM.

In Vivo

CUR5g (40 mg/kg, Injected via caudal vein, once every 2 days for up to 15 days) exhibits synergistic anticancer effects with Cisplatin (HY-17394) (1 mg/kg) and inhibits autophagic flux in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude mice (4-week-old, A549 cells were subcutaneously injected into the right scapula of each nude mouse)^[1]
Dosage:

40 mg/kg, CUR5g (40 mg/kg) and Cisplatin (1 mg/kg)
Administration:

Injected via caudal vein, once every 2 days for up to 15 days
Result:

Retarded the growth of xenografted tumors, whereas the combination treatment with Cisplatin almost completely inhibited tumor growth. Promoted the cisplatin sensitivity of A549 cells by inhibiting autophagic flux.

Chemical Information

CAS. Nr. 1370032-20-4
Appearance Solid
Molecular Weight 344.41
Formel C₂₂H₂₀N₂O₂
Color Yellow to brown
SMILES

O=C1/C(CN(C)C/C1=C\C2=CNC3=C2C=CC=C3)=C/C4=CC=C(O)C=C4
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 41.67 mg/mL (120.99 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9035 mL

14.5176 mL

29.0352 mL

5 mM

0.5807 mL

2.9035 mL

5.8070 mL

10 mM

0.2904 mL

1.4518 mL

2.9035 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Chen J, et al. CUR5g, a novel autophagy inhibitor, exhibits potent synergistic anticancer effects with cisplatin against non-small-cell lung cancer. Cell Death Discov. 2022 Oct 31;8(1):435. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9035 mL	14.5176 mL	29.0352 mL	72.5879 mL
	5 mM	0.5807 mL	2.9035 mL	5.8070 mL	14.5176 mL
	10 mM	0.2904 mL	1.4518 mL	2.9035 mL	7.2588 mL
	15 mM	0.1936 mL	0.9678 mL	1.9357 mL	4.8392 mL
	20 mM	0.1452 mL	0.7259 mL	1.4518 mL	3.6294 mL
	25 mM	0.1161 mL	0.5807 mL	1.1614 mL	2.9035 mL
	30 mM	0.0968 mL	0.4839 mL	0.9678 mL	2.4196 mL
	40 mM	0.0726 mL	0.3629 mL	0.7259 mL	1.8147 mL
	50 mM	0.0581 mL	0.2904 mL	0.5807 mL	1.4518 mL
	60 mM	0.0484 mL	0.2420 mL	0.4839 mL	1.2098 mL
	80 mM	0.0363 mL	0.1815 mL	0.3629 mL	0.9073 mL
	100 mM	0.0290 mL	0.1452 mL	0.2904 mL	0.7259 mL

Keywords

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

CUR5g

Anti-Cancer Compound Library

Autophagy Compound Library

Cysteine Targeted Covalent Library

Highly Selective Inhibitors Library

Cell Death Library

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Brain Cancer Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

CUR5g

Anti-Cancer Compound Library

Autophagy Compound Library

Cysteine Targeted Covalent Library

Highly Selective Inhibitors Library

Cell Death Library

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Brain Cancer Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

CUR5g Related Classifications

Art. -Nr.: HY-152100