Cyclamic acid
Based on 1 Customer Validation
Cyclamic acid (Cyclamate) is a commonly used sweetener. Cyclamic acid sodium is toxic to osteoblasts and can inhibit cell proliferation, induce apoptosis and reduce cell mineralization. Cyclamic acid sodium causes focal necrosis of bladder organs in rats in vitro, which can promote bladder cancer, but some studies have shown that low doses of Cyclamic acid sodium have no carcinogenic effect. In addition, Cyclamic acid sodium has no effect on insulin and glucagon secretion induced by arginine.
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- Reinheit: 99.79%
- CAS. Nr.: 100-88-9
- Formel: C6H13NO3S
- Molecular Weight:179.24
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Orally Active Compound Library
HY-L061
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Human Metabolite Library
HY-L123
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Osteogenesis Compound Library
HY-L131
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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HY-L001V
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Biologische Aktivität
Cyclamic acid (0-0.1 μM, 24-72 h) causes the apoptosis of osteoblast cells and shows significant inhibition of osteoblasts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Osteoblast cells
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Concentration:0-0.1 μM
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Incubation Time:24-72 h
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Result:Resulted death of more than 50% osteoblasts after 72 h's treatment.
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Cell Line:Osteoblast cells
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Concentration:0-0.1 μM
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Incubation Time:24-72 h
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Result:Decreased the expression of BMP2 and reduced the mineralization capacity of osteoblasts by lowing the expression of ALP.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Dogs[3]
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Dosage:2-4 g/kg
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Administration:p.o., daily for 2 weeks
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Result:Occurred diarrhea in dogs.
Chemical Information
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CAS. Nr. 100-88-9
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Appearance Solid
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Molecular Weight 179.24
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Formel C6H13NO3S
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Color White to off-white
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SMILES
O=S(O)(NC1CCCCC1)=O
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Synonyms
Cyclohexylsulfamic acid; Cyclamate
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Lösungsmittel & Löslichkeit
H2O : ≥ 100 mg/mL (557.91 mM)
DMSO : 100 mg/mL (557.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Reinheit & Dokumentation
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Data Sheet (296 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Leban I, et, al. Structures of artificial sweeteners--cyclamic acid and sodium cyclamate with other cyclamates. Acta Crystallogr B. 2007 Jun;63(Pt 3):418-25. [Content Brief]
[2]. Chen Z, et al. Toxicity of food sweetener-sodium cyclamate on osteoblasts cells. Biochem Biophys Res Commun. 2019 Jan 8;508(2):507-511. [Content Brief]
[3]. Bopp BA, et al. Toxicological aspects of cyclamate and cyclohexylamine. Crit Rev Toxicol. 1986;16(3):213-306. [Content Brief]
[4]. Usami M, et al. Effect of cyclamate sodium, saccharin sodium and stevioside on arginine-induced insulin and glucagon secretion in the isolated perfused rat pancreas. Horm Metab Res. 1980 Dec;12(12):705-6. [Content Brief]
[5]. Knowles MA, et al. Induction of morphological changes in the urothelium of cultured adult rat bladder by sodium saccharin and sodium cyclamate. Carcinogenesis. 1986 May;7(5):767-74. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 5.5791 mL | 27.8956 mL | 55.7911 mL | 139.4778 mL |
| 5 mM | 1.1158 mL | 5.5791 mL | 11.1582 mL | 27.8956 mL | |
| 10 mM | 0.5579 mL | 2.7896 mL | 5.5791 mL | 13.9478 mL | |
| 15 mM | 0.3719 mL | 1.8597 mL | 3.7194 mL | 9.2985 mL | |
| 20 mM | 0.2790 mL | 1.3948 mL | 2.7896 mL | 6.9739 mL | |
| 25 mM | 0.2232 mL | 1.1158 mL | 2.2316 mL | 5.5791 mL | |
| 30 mM | 0.1860 mL | 0.9299 mL | 1.8597 mL | 4.6493 mL | |
| 40 mM | 0.1395 mL | 0.6974 mL | 1.3948 mL | 3.4869 mL | |
| 50 mM | 0.1116 mL | 0.5579 mL | 1.1158 mL | 2.7896 mL | |
| 60 mM | 0.0930 mL | 0.4649 mL | 0.9299 mL | 2.3246 mL | |
| 80 mM | 0.0697 mL | 0.3487 mL | 0.6974 mL | 1.7435 mL | |
| 100 mM | 0.0558 mL | 0.2790 mL | 0.5579 mL | 1.3948 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Cyclamic acid Related Classifications
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